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In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

本文采用RT—PCR技术,研究了中华鳖不同组织Sox基因的表达,并通过PCR直接克隆法,分析了来自睾丸、脑和脾组织中的Sox基因序列结果表明在中华鳖的成体组织中,Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达,而在肌肉、肝脏和雌性的卵巢中则无表达,显示该基因具有组织表达特异性克隆分析显示,在睾丸组织中表达的是TSSox1和TSSox4基因,而在脑组织中表达的是TSSox2和TSSox4基因,脾组织中表达的是TSSox4基因此结果表明Sox基因不仅在性别决定中起作用,还可能在神经系统、免疫系统多种组织中起重要作用

The model test and the analysis of a 3-span curved unbonded prestressed concrete box-girder are presented.

在第四章中,介绍了一例无灌浆的预应力混凝土曲线连续箱梁的模型试验结果和理论计算结果。

The mc2155 carrying pMind-glmU-AS was induced by different concentration of tetracycline, and GlmU protein was detected in the mc2155 carrying pMind-glmU-AS by Western blotting. The results showed that tetracycline of 20ng/ml has obviously inhibited the growth rate of mc2155 carrying pMind-glmU-AS, but the amount of GlmU protein did not change compared to that of uninduced mc2155 carrying pMind-glmU-AS.

glmU反义RNA的诱导表达与GlmU蛋白的检测分别用5 ng/ml、10 ng/ml和20 ng/ml的四环素诱导携带pMind-glmU-AS表达载体的mc~2155菌株表达反义RNA,绘制细菌生长曲线,结果表明20 ng/ml浓度的四环素可抑制mc~2155/pMind-glmU-AS的生长,但Western Blotting分析结果显示经四环素诱导和未诱导的mc~2155/pMind-glmU-AS中,GlmU蛋白表达量无明显的变化。

To identify the non-uniform coating in a carbon fiber tow can be overcome by the pneumatic spreading process; two groups of spread and unspread carbon fiber tow coated with nickel by electroless plating were compared.

为了证明藉由气体展开制程可克服碳纤维纱束不均匀镀层的结果,将碳纤维纱束分为展开与未展开两组,比较无电镀镍后的结果。

Abstract] objective to evaluate the distinctive clinical value of magnetic resonance urographymru)in the diagnosis of primary ureteral carcinoma.methods to compare the accuracy of diagnosis of mru with ultrasonography,urography,retrograde ureteropyelography and ct,diagnostic imaging and progress of ureteral carcinoma were analyzed in twenty-four cases from 2000 to 2006,twenty cases had mru examinations.the original preoperative diagnostic reports of various imaging facilities were analyzed on account of the results of operation and pathology.results mru could clearly show the obstructive location of ureter.with regard to the ureteral carcinoma,accuracy of the location diagnosis was 100%.mru was superior to ulstrasonography,kub+ivp,ureteropyelography and ct we used to apply.conclusion comparing with other diagnostic imaging facilities,it has an important value in highly efficient,accurate location,safe,noninvasive and so on with mru,which can be used to show the features of primary ureteral carcinoma and has a wider scope of clinical application.

目的 探讨磁共振水成像技术在原发性输尿管肿瘤中的临床诊断价值。方法分析2000~2006年24例原发性输尿管肿瘤的影像学诊断结果,并基于术中所见和病理报告,将磁共振水成像技术20例)与超声24例)、泌尿系造影24例)、逆行造影15例)、ct(19例)的确诊率相比较。结果 mru能清晰显示输尿管的梗阻部位,定位诊断准确率为100%,梗阻病因确诊率高于b超、泌尿系造影、逆行造影和ct检查。结论与其他影像诊断方法相比较,磁共振水成像在显示原发性输尿管肿瘤特征方面具有效率高、定位准确和安全无创等重要价值,而且有更广泛的临床应用范围。

Through optimizing the parameters of the impact mechanism and using of the result above, the optimum result on the impact mechanism is obtained, which has had practical consult value to design valveless hydraulic drill.

利用仿真研究的结果,对影响冲击机构的物理参数和结构参数进行了优化,得出了优化结果,对于无阀液压凿岩机的设计具有实际参考价值。

The "yes" or "no"(1 or 0) of distribution of reptiles in Guangdong Province were regarded as two-state character. The relative coefficient was taken for the similar degree of reptiles in every two zoogeographical provinces. The cluster analysis was taken by group average method. The characteristics of geographical distribution and the comparison between the result of cluster analysis and the geographical distribution of reptiles in Guangdong Province are discussed.

将各种爬行动物在广东4个动物地理省(粤北、粤东、粤西、沿海)分布的有或无作为二元性状,用联合系教来表征每两个动物地理省爬行动物组成的相似程度,以类平均法进行聚类分析,结果表明爬行类在广东各动物地理省的分布受地形明显影响,论述了广东省爬行动物地理分布的特点,并对聚类结果与动物地理区划进行了比较。

MAIN OUTCOME MEASURES: The amount of trypan blue absorbed by the CAM. RESULTS: The correlation coefficient between CAM-TB score and Draize eye irritation was 0.925 9. Glycerol had no eye irritation potential, while acet, alcohol, methanol and DMSO had.

结果:鸡胚尿囊绒膜-锥虫蓝吸收法与体内兔眼刺激实验评分结果相关性很好(R2=0.925 9)。5种常见有机溶剂未稀释时,甘油无眼刺激性,丙酮、乙醇、二甲基亚砜、甲醇均有较高刺激性。

RESULTS: All 15 patients were involved in the result analysis without any drop.

结果:15例患者全部进入结果分析,无脱落。

But, under basic offence no result of death, absconding causing result of death is complex aggravated offences with the conduct and result factor.

其中,在基本罪无死亡结果情形下的&逃逸&又导致死亡结果时,为基本罪的复合加重犯。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。