无结果

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The results indicate that a regular diffractive facular lattice will appear in the maximum diffraction-free distance with the increase of the oblique incident angle.

结果指出，随着入射轴棱锥角度的增大，在最大无衍射距离内会形成有规则的光学点阵，这种光学点阵与三维光子晶体的结构相近，实验结果与数值模拟相吻合。

Result and Discussion: Participant with shoulder pain had significantly decreased acuity than that in participant without shoulder pain, especially internal rotation and horizontal abduction in glenohumeral joint.

结果与讨论：结果显示肩痛者比无肩痛者在肩胛胸廓、肩盂、以及胸锁三个关节都有著较高的三维变异误差，代表受伤组织使得肩部各关节在整体动作中的准确性皆下降，尤其在肩盂关节两者的差异最大。

The microstructure of MDF and MDF tested were studied. The results showed that the fibers in MDF are arranged randomly. Some of them arranged separately, most of them arranged in bundles. There are many blind holes between the fiber cells in MDF. The damage of MDF's compressive creep is the result of new cranny occuring, spreading and the combination of old cranny. In the repressing process, besides the pressure, heat is also the main factor on the damage of MDF, which can accelerate the adhension layer to deteriorate and lead to the gule strength decreasing.

结果表明：MDF的纤维排列无规则，纤维排列形态除少量纤维呈单根状外，大量的纤维以纤维束的形态排列；纤维之间存在许多大小不等的孔洞，呈不贯通状；MDF蠕变损伤是不断萌生新的裂隙并扩展和合并原有裂隙的结果；MDF二次加工过程中，除了压力是破坏MDF结构的主要因素外，加热温度使MDF胶层老化，胶合强度下降，也是破坏MDF结构的主要因素；MDF的破坏形态除了有纤维拉断外，还有纤维拔出和胶粘剂脱落。

RESULTS Pe-ripheral whole blood cells of this experimental rabbit re-duced,and by PCR,no donor's cell was found,and slight defective profileration of marrow occurred;the pathologic re-sults showed that its pathological processes mainly occurred in its skin and were all slighter than homogenic GVHD,and there was no obvious difference in the invaded regions be-tween heterogenic and homogenic GVHD.

结果 该实验兔外周全血细胞减少，PCR检查未发现供体细胞，骨髓轻度增生不良；病理结果显示其病变仍以皮肤为重但均较同种GVHD轻，其病变侵犯部位与同种GVHD无明显区别。

Results:There was no significant difference in mensuration between microwave disgestion-acetylacetone-formaldehyde spectrophotometry method and international standard Kjeldahl determination.

结果：微波消解-乙酰丙酮甲醛分光光度法与国标凯氏定氮法相比测定结果差异无显著性，该方法线性范围在5~100μg/10 m l，相关系数r=0.9997，检出限为0.546μg/10 m l，相对标准偏差为1.29%~2.21%，加标回收率

In result 2.5% of the rats got slight hepatic enzymes increase, and 6.25% of the rats got slight fat degeneration for hepatic tissue specimens. Most of the rats with abmormal results occurred in the toxic and lethiferous group, and there was the increasing tendency with the increase of dose. There were no changes for renal function and tissue biopsy.

实验结果显示共有2.5％的小鼠出现了轻度的肝酶升高，组织病理有6.25％的小鼠肝组织出现了轻度的脂肪变性，结果异常的小鼠绝大多数发生于中毒剂量及致死剂量组，且有随着剂量增高而增加的趋势；肾功能及组织病理无明显改变。

Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P＜0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P＜0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P＜0.05)，且各剂量组微核率随染毒浓度的增加而升高，呈现剂量-反应关系(r=0.937，P＜0.01)；中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P＜0.05)，且各剂量组精子畸形率随染毒浓度的增加而升高，呈剂量-反应关系(r=0.904，P＜0.01)，畸形精子中以无钩为最多，其次是香蕉形、尾折叠、双头，分别为4.5%、3.09%和1.32%，结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P＜0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P＜0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P＜0.05)，且各剂量组微核率随染毒浓度的增加而升高，呈现剂量-反应关系(r=0.937，P＜0.01)；中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P＜0.05)，且各剂量组精子畸形率随染毒浓度的增加而升高，呈剂量-反应关系(r=0.904，P＜0.01)，畸形精子中以无钩为最多，其次是香蕉形、尾折叠、双头，分别为4.5%、3.09%和1.32%，结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

Results: The contents determined with microtitration were 98.73% and that of regular titration was 98.71%.

结果：微型滴定和常规滴定分别测得马来酸氯苯那敏的含量为98.73％和98.71％，平行测定结果差异无统计学意义。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。