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Apparently, the results is determined extremely by theoretical algorithm. In 147 zinc-finger-containing genes, 50nt identity sequences were extracted. The results hint that 15 genes may related to block evolution. we analyzed the phylogenetic map of these 15 genes by uncorrected distance, Jukes Cantor distance, Tamura distance, Tajima-Nei distance, Jin-Nei distance or Kimura distance correction, but we found no satisfied result according to the block exchange pattern mentioned before. Introducing Bootstrap test to assistant correction, an comparatively perfect map was drown by Neighbor Joining method.

以147个含锌指基因为模板，我们搜索了其中的50nt一致序列作为板块式进化的核心，获得15个相关基因，应用无校正距离、Jukes Cantor校正距离、Tamura校正距离、Tajima-Nei校正距离、Jin-Nei校正距离和Kimura校正距离等分析了他们之间的进化关系，结果与已知的板块交换模式吻合度较差，引入靴带检验对Jukes Cantor校正距离结果进行统计学校正，并重新用Neighbor Joining方法绘制进化图，结果可靠性大大提高。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

Based on the method of finite element orthogonal test,this paper designs the experimental scheme and gives numerical model of top coal drawing with non chain pillar entry protection .

根据有限元正交试验法，设计了有限元正交试验方案，建立了放顶煤无煤柱护巷的数值模型；通过对计算结果分析，得出放顶煤无煤柱开采沿空巷道的布置参数和支护形式，可为放顶煤无煤柱护巷提供理论依

Relating ICF scores with TBI variables, it was found that disability classification and Body Function total score are statistically associated; Activities and Participation total score is significantly related to both disability classification and Glasgow Coma Score; convulsion, amnesia and neurological deficients are significantly associated with the Environmental Factors total score.

将ICF总分与外伤性脑损伤基本资料之各变项分析结果，身体功能总分与身心障碍等级有关；活动与参与总分与身心障碍等级及格拉斯哥昏迷指数有关；环境因子总分与有无痉挛、有无健忘及有无神经障碍3个变项有关。

Results The average age of the patients was raised, the advanced age and high risk patients increased, the therapy time from chest pain onset to emergency visit, from emergency visit to catheter lab and from catheter lab to the first balloon angioplasty shortened, and the use of drug-eluting stent, distal protective device,aspiration thrombectomy catheter, injection of glyceryl trinitrate and diltiazem to the coronary artery became more frequent during recent 6 years.

结果平均年龄增大、高龄及高危患者增多，治疗时间窗中各时间段（发病→急诊科→导管室→首次球囊扩张）均有缩短，其中导管室→首次球囊扩张时间缩短显著；药物支架、远端保护装置、血栓抽吸器、预防性冠状动脉联合注射防止无复流药物在近两年增长迅速，无血流发生率、主要心血管事件发生率及平均住院日显著下降，同时溶栓后介入治疗较直接介入治疗各种严重出血并发症无明显增加。

Significant differences were shown in the observation times of females during the peak and the middle of the reproductive season at different densities. The observation time of the females was much longer in low density population than that in high density population. For males, there was no significant difference in observation time between or among population densities. At both low and high population densities significant seasonal variation was found in the duration and frequency of male plateau pikas observation behavior, nor was that of females.

结果表明：在繁殖盛期和繁殖中期，低密度种群内雌性高原鼠兔的观望时间均显著高于高密度种群内，而在其它两个时期内无明显不同；雄性高原鼠兔的观望时间在不同密度间比较均无显著差异；同一密度条件下，雄性高原鼠兔的观望行为持续时间和发生频次均表现显著的季节性变化，而雌性高原鼠兔无明显差异。

The test results were then independently reviewed by the Society of Automotive Engineers and published as an SAE paper, before the formula was finalised and finally released to petrol stations as New Shell Unleaded, Shell Unleaded 95 and Shell Premium Unleaded petrols.

测试结果，发表在独立审查的汽车工程师学会和一个汽车工程师学会发表论文，在公式最后定稿之前，公布在加油站的无铅新壳牌，壳牌无铅95和壳牌高级无铅汽油

objective:to explore the effects of out cavity single slip knot technique in non titanium clip two hole method laparoscopic cholecystectomy.methods:to analyzed the clinical data of 62 patients underwent two hole method lc with out cavity single slip knot technique.results:all the operations were finished successfully without conversion.the mean operation time and hospitalization were 55 min and 4d respectively.conclusions:the two hole lc with out cavity single slip knot technique has the advantage of firm and reliable ligation,low operation expense,non titanium clip stay in the body,and avoiding of bile leakage due to titanium clip falling off.

目的：探讨腔外单滑结技术在无钛夹二孔法腹腔镜胆囊切除术中的效果。方法：应用腔外单滑结技术行二孔法lc 62例。结果：患者均行lc，无中转开腹，手术平均时间55min，平均住院4d。结论：无钛夹腔外单滑结法lc具有结扎可靠，手术费用低，并可消除因钛夹留置体内或夹闭不全造成脱落、胆漏等其他并发症。

Namely, with advancing growth stage, Changes of nutritional compositions in range and velocity for first-cut alfalfa were bigger than those for second-cut alfalfa.

不同茬次初花期的苜蓿养分比较结果为：第一茬苜蓿的消化率、粗蛋白、无氮浸出物含量显著高于后两茬(P＜0.05)，而在第二茬与第三茬之间无显著性差异(P＞0.05)；CF、NDF、ADF含量第二茬最高，第一茬和第三茬无显著性差异(P＞0.05)；CASh含量第一茬较高；各茬次中EE、Ca、P含量较小，变化不大。

The development of the lead free cutting brass containing bismuth and graphite/copper composite was summarized.It can be concluded that the key points for cutting brass containing no lead are the choices of elements which can form brittle but not hard intermetallics and procedure which can make the intermetallics distr...

详细介绍国外无铅易切削铋黄铜研究开发的一些成果和石墨/铜合金复合材料的研究情况，总结国内无铅易切削铜合金的研究情况，结合自己的研究结果，指出无铅易切削铜合金的研究方向是采用适当的元素形成脆而不硬的金属间化合物，并采用一定的加工手段使其呈弥散分布。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。