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Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损,骨刀切除软骨下骨到髓腔渗血为止(厚约4mm),压迫后FG止血;取髂骨骨块,并尽可能保留松质骨,取下的骨块用PBS反复清洗,以除去血细胞,将松质骨填充在骨缺损处,松质骨面朝向关节腔,高度与周边软骨下骨齐平,把骨膜片生发层朝向关节腔,用无创伤缝合线缝合在周边的软骨或滑膜组织上;向EP管中加入1/2悬液体积的FG主体胶溶液并混匀,再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中;同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞;C组造成骨软骨缺损,FG覆盖创面后单纯用骨膜修复缺损。

Results: The differentiated neuron appeared different patterns of amitotic figures such as transverse sever, longitudinal split, etc. The proliferating neurons divided in an asymmetric way morphologically and chemically. The differentiated neuron appeared an obvious movement of cell migration and neurite extension.

结果:培养分化的神经细胞呈现横裂、纵裂等不同方式的无丝分裂像;分裂中的神经细胞可呈现形态及化学不对称性;分化的神经细胞有明显的细胞迁徙运动和突起延伸。

The leaf anatomical structures of the mosses are of ecological adaptation to their habitats. The mosses wider dry conditions often have thickened laminal cell-walls on the surfaces, and some of them have appendiculate structures.

藓类植物叶的解剖结构具有生态适应意义,旱生环境下的藓类植物,叶片细胞胞壁具不同程度的增厚,有些藓类植物叶片具附属结构,藓类植物中肋的有无,反映了对水分吸收和运输方式的不同。

Results The negative correlations were found on the Glasgow Outcome Scoreto the items such as conscious state at admission,Glasgow Coma Score,psychiatric symptoms,hemiplegia,Babinski sign,abnomal degree of Electroencephalogram,focal lesions found by neuro-image investigation and mean peak flow velocityand pulsatility indexof basilar artery and vertebral arteries by transcranial Doppler ultrasonography investigation;The non-correlation was found on the Glasgow Outcome Score to theitems such as headache,febrile,epilepˉsia style,the involvement of cranial nerve,dystaxia,neck stiffness,Cerebrospinal fluid investigation and blood serum enzymology.

结果 入院时的意识状态、GCS、精神症状、偏瘫和/或偏身感觉障碍、巴彬斯基征以及脑电图异常程度、影像学所见的病灶范围、基底动脉和椎动脉的平均峰流速、脉动指数和Glasgow预后评分呈负相关;头痛、发热、癫痫类型、颅神经受累情况、共济运动、脑膜刺激征、脑脊液检查(含压力、蛋白定量、细胞数、HSV-PCR)以及血清酶学的变化和Glasgow预后评分之间无相关性。结论入院时的意识状态、GCS、精神症状、局灶性神经系统体征以及脑电图异常程度、影像学所见的病灶范围、后循环的血管痉挛程度和血流阻力等是影响病脑预后的主要因素。

Results The negative correlations were found on the Glasgow Outcome Scoreto the items such as conscious state at admission,Glasgow Coma Score,psychiatric symptoms,hemiplegia,Babinski sign,abnomal degree of Electroencephalogram,focal lesions found by neuro-image investigation and mean peak flow velocityand pulsatility indexof basilar artery and vertebral arteries by transcranial Doppler ultrasonography investigation;The non-correlation was found on the Glasgow Outcome Score to theitems such as headache,febrile,epilepˉsia style,the involvement of cranial nerve,dystaxia,neck stiffness,Cerebrospinal fluid investigation and blood serum enzymology.

结果 入院时的意识状态、GCS、精神症状、偏瘫和/或偏身感觉障碍、巴彬斯基征以及脑电图异常程度、影像学所见的病灶范围、基底动脉和椎动脉的平均峰流速、脉动指数与Glasgow预后评分呈负相关;头痛、发热、癫痫类型、颅神经受累情况、共济运动、脑膜刺激征、脑脊液检查(含压力、蛋白定量、细胞数、HSV-PCR)以及血清酶学的变化与Glasgow预后评分之间无相关性。结论入院时的意识状态、GCS、精神症状、局灶性神经系统体征以及脑电图异常程度、影像学所见的病灶范围、后循环的血管痉挛程度和血流阻力等是影响病脑预后的主要因素。

The tumor is sessile, polypoid, or castlike and is characterized by the presence of innumerable papillary frondlike infoldings comprised of proliferations of columnar epithelial cells around the slender fibrovascular stalks that are supported by the connective tissue of the lamina propria.1 Occasionally, tumor spreads superficially along a variable length of the bile duct.

该肿瘤是无蒂、乳头样或脱落物样的,以及肿瘤表面特征性地存在无数乳头样叶状内折叠,这些乳头是由围绕纤细的以管腔的结缔组织为支撑的纤维血管茎增殖的柱状上皮细胞构成。肿瘤偶尔也会沿不同长度在胆管表面蔓延。

And more, ERK contents between group A and B, group C and D were not dramatically different. The pathological lesions indicated that intestinal tissue necrosis was severe in group C and D, which were graded 3 points, but obviously lessened in group A and B, which was graded 1 point, with ITF interfering. Conclusion: Intestinal inflammation was ameliorated after ITF was injected hypodermically or intraperitoneally.

结果:A、B组组织匀浆中ERK的面密度值各为87.68±19.24、82.65±21.35,较C、D组(30.23±7.79、30.74±8.19)明显升高(P<0.01),C、D组与E组(5.41±1.46)比较也有升高(P<0.05);并且随著ERK的激活伴随著明显的核转位。A、B组间及C、D组间ERK含量无显著差异(P>0.05);病理切片显示C、D组HE染色切片见肠壁损伤轻重不一,可见全肠粘膜绒毛坏死,病理评分的中位积分为3分;A、B组肠上皮细胞少量脱落,顶端绒毛坏死,病理评分的中位积分为1分。

The Immunohistochemical results indicated that the pI〓 is obviously located on the liminal surface membrane of the distal tubules and Henle's ascending limbs, and can also be found at Henle's loop of porcine kidneys, these segments expose in non-isotonic condition.

免疫组织化学染色显示,pI〓在猪肾脏明显定位于非等渗区域的远曲小管和髓袢升支粗段的管腔表面膜及髓袢处,而等渗区域的近曲小管细胞几乎无pI〓的存在,据此认为pI〓可能在耐受渗透压的变化上发挥作用。

Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6/ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片,分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化,即方法1先用含bFGF的培养基进行预诱导,之后再用含叔丁基对羟基茴香醚,和二甲亚砜的无血清培养基进行诱导;方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导,然后不同浓度的上述物质进行正式诱导。

Methods: Serum-free conditioned media of Schwann cell cultures from facial and sciatic nerves of neonatal and Wallerian degenerating segments in adult rats were individually collected and concentrated by ultra-filtration with molecular weight cut-off at 30 000 and 10 000. The growth activities of FMNs in vitro were determined by means of MTT assay under the condition of serum-free medium added with different components of concentrated SC-CMs.

收集1~4代大鼠沃勒变性段和乳鼠面神经及坐骨神经SC体外无血清条件培养液,超滤浓缩,测定分离纯化的FMN细胞在含不同来源的SC分泌的可溶性物质的条件培养液中的生长活性。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。