无细胞的

NADPH oxidase inhibitor, diphenylene iodonium chloride (DPI, 10μmol/L), abolished histamine-stimulated calcium oscillations both in normoxic and posthypoxic PAECs.(3) Xanthine oxidase inhibitor, oxypurinol (100μmol/L), did not affect the calcium oscillation frequency in normoxic PAECs. However, it significantly decreased the elevation of calcium oscillation frequency in posthypoxic PAECs.

黄嘌呤氧化酶抑制剂，别嘌呤醇(oxypurinol， 100μmol/L)能显著降低组胺刺激的缺氧后肺动脉内皮细胞升高的钙振荡频率，但降低后的钙振荡频率仍高于常氧组，别嘌呤醇对组胺刺激的常氧组肺动脉内皮细胞钙振荡频率无显著影响。

Methods Thirty eight patients with loosening of hip prosthesis were divided into three group: total hip arthoplasty, double hip arthuplasty and integrated arthoplasty. The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph, and the content of TNF in samples of interface membranes was determined.

方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分为3组：全髋置换组、双动头组和一体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计数的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

Chitosan and pigskin were added into the PLGA conduit of film containing gelatin microsphere (containing bFGF, then this material was planted into rabbit's ischiadic nerve. The experiments indicate that the material has a good biocompatibility, no inflammatory reaction, and no allergic response.

将成功构建的含bFGF明胶微球与PLGA材料加入壳聚糖、粉碎的脱细胞猪皮等改善其机械性能制作成复合导管之后，将此导管植入兔坐骨神经进行相关的观察，初步实验显示此材料在兔坐骨神经部位生物相容性良好，无炎症反应，无过敏反应。

High proportion of cells was in Sub-G1 phase of cell cycle and the cells could not grow in soft agar to form colonies. On the contrary, the three breast cancer cell lines, Bcap-37, MCF-7 and MDA-MB-231, are anoikis resistant, as indicated by DNA laddering and FCM assays.

而Bcap-37、MCF-7、MDA-MB-231三种乳腺癌细胞则具有抗脱落凋亡的特性，分别与它们各自贴壁培养的细胞比较，胞浆DNA琼脂糖凝胶电泳表现无明显差异，FCM所检测到的凋亡峰也无明显区别。

Each group of rats was kept in a separate cage. All of the rats drank water freely and were fed on stand food for rats.The experiment lasted 4 weeks. Indexes to be examined are as follows:⑴spleen T lymphocyte transform value,⑵spleen NK cell activation alternation value,⑶abdominal cavity macrophagocyte IL-1 and IL-2 activation alternation value.

所有动物分笼饲养，各组均自由饮水，喂食标准鼠类饲料，实验时间为4周。4周后，ME组和FE组作一次性力竭游泳运动后和其他各组大鼠股动脉放血处死，留取血清，无菌取胸腺和脾脏，行以下指标检测：⑴脾脏T淋巴细胞转化值；⑵脾脏NK细胞毒活性的变化值；⑶腹腔巨噬细胞IL-1的活性变化值和脾脏分泌的IL-2的活性变化值。

Counting method of microorganisms in environment;②preparation of smears for observing forms and structures of bacteria、Actinomyces、yeasts and molds, methods and technologies of staining and oil immersion objective;③cell counting techniques and microscope examination techniques;④preparation of microbic media and sterilization techniques;⑤aseptic operations and microbic inoculation methods ;⑥technics of microbic separation、purification and cultivation;⑦physiological and biochemical reactions of microorganisms and the microbial examination techniques of food sanitation to strengthen students the operation techniques.

课程内容：本课程实验内容包括：①环境中微生物检测计数；②细菌、放线菌、酵母及霉菌细胞形态、结构的制片，染色和显微镜观测，油浸物镜的使用技术；③细胞计数技术和显微测量技术；④微生物培养基制备、压力蒸气灭菌技术；⑤无菌操作技术、移种技术；⑥微生物分离、纯化和培养技术；⑦微生物的生理、生化反应及食品卫生微生物检测技术等旨在加强学生微生物学的操作技术。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

Low reaction of fetus to inflammation, different extracellular matrix, component of collagen fiber and cell factors may take part in scarless healing process. Regulation of gene expression may play an important role in the process. But the concrete mechanism of scarless healing is not clear yet and still needs more researches and elucidations.

胎儿对炎症的低反应性、胎儿不同的细胞外基质、胶原纤维的成分、细胞因子可能也参与了无瘢痕愈合过程、基因表达调控在其过程中可能也起重要作用，但无瘢痕愈合的具体机制还不明了，有待进一步研究阐明。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

Result:The stomatic density and characteristic of leaf epidermis and stem epidermis in six Ephedra species was differenc,there were no ob...

结果：6种麻黄属植物之间气孔密度、茎、叶表面特征存在不同程度的差异；气孔表现出典型旱生植物的特征，略下陷，气孔保卫细胞被厚的角质层覆盖，并形成突起状角质唇状物；气孔大小、形状在种之间无明显区别；叶表面、茎表面气孔均属于无规则型，但形状明显不同，前者主要呈长方形或六边形，后者为窄条形或哑铃形。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。