无细胞的

Under suspension culture without cytokines, the putative EG clonies could be induced to form simple embryoid bodies.

将消化分散的类EG细胞在无分化抑制因子的类EG培养基中重悬培养，3～4天后，可见部分细胞团形成简单的胚体。

The two-dimensional gel electrophoresis (2D) analysis followed by fluorography clearly displayed proteins preferentially methylated in PRMT1-expressing cell lysates suggesting that they were potential PRMT1 substrates.

在此研究中我们建立大量表现PRMT1 (protein arginine methyltransfrase 1)的细胞模式，并成功地以二维胶体电泳展开甲基化蛋白，发现有无大量表现PRMT1的HEK293细胞，在一些蛋白质甲基化作用的情形有明显差异。

RBL-2H3 cells expressing formyl peptide receptor being stimulated with two polypeptides was used as model system to characterize the chips' performance. As a result, fMLF triggered chemotaxis, receptor internalization and calcium mobilization, while GSH had no effect on the cells.

以含有不同氨基酸序列的多肽作用表达甲酰肽受体的RBL-2H3细胞为模型体系，对芯片组性能予以验证，结果表明，N-甲酰肽物质能够刺激细胞发生趋化运动、受体内吞和钙离子释放，而作为阴性对照的还原型谷胱甘肽无此能力。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

Results Among16826 subject cases,1 case of squamous cell caroinoma has been found,2cases of CIN-3,12cases of CIN-2,and391 cases of CIN-1 have been found And among the found cases,there are263 caese of CIN-1 case of HPV infection;197cases of trichomonal infection;388cases of candidal infection,156 cases of bacterial infection .14410cases of inflammatory cell changing;2001 cases of ceriical intraepithelial neoplasia,8cases of ASC-US and1 case of AGUS.

结果 16826例标本中，发现1例鳞状细胞癌；2例CIN-3；12例CIN-2；391例CIN-1，其中263例 CIN-1HPV感染；滴虫感染197例；霉菌感染388例；细菌感染156例；有炎性反应性细胞改变的14410例；无上皮内病变或恶性瘤变的2001例；8例ASC-US；1例AGUS。

Ethods Tissue specimens were stained immunohistochemically for MMP-2 and MMP-9 in 30 cases of squamous cell cervical carcinoma,29 cases of cervical intraepithelial neoplasia and 16 cases of normal cervices.

MP- 2鳞癌组与CIN组及正常组相比差异有极显著性(P 。0 1)。 MMP- 9正常组与鳞癌组及 CIN组相比差异有显著性(P 。0 5 )。MMP- 2在各组间质的基质细胞中表达均为阳性。MMP- 9在正常宫颈组织、CIN及鳞癌的基质细胞中阳性表达分别为 0 / 16 (0 %)；4/ 2 9(13.8%)；7/ 30 (2 3.3%)。各组间相比差异无显著性。

The results presented in this paper indicated that sodium salt of 3,7-bishemis uccinate of 7-hydroxycholesterol (RS 634) at the dose of 45mg/kg/day (×7,ip) could increase DNA, RNA and protein syntheses in bone marrow cells and protein synthesis in liver,spleen and serum, but no markedly effects on DNA and RNA synthesis in liver and spleen of mice bearing EAC were observed, In the subacute test on rats RS 034 (400mg/kg/day, x30 ip) showed no evident effects on the number of RBC, WBC and the percentage of lym...

本文报告的结果表明7β-羟基胆固醇双琥珀酸单酯钠(RS 024)在有效抗肿瘤剂量下(45mg/kg/day，×7ip)对荷瘤小鼠骨髓细胞DNA、RNA和蛋白质合成有促进作用，对肝、脾的DNA、RNA合成无显著影响，但能促进肝，脾中及血清蛋白质合成。按400mg/kg/day连续给药30天，对大鼠红细胞、白细胞数及淋巴细胞百分率无明显影响。提示该药在有效抗癌剂量下毒性较小，并在一定程度上对免疫功能有增强作用。

The green fluorescence could be observed in transfected cardiomyocytes, but not in transfected myoblasts.

成功转染mhc-egfp表达盒的心肌细胞出现绿色荧光，而转染的骨骼肌成肌细胞无荧光出现。

Compared with the control group, the concentration of IFN-γ, of rats in mental stress group is higher significantly (P＜0.05) than that in the control group, but the concentration of IL-4 has no change.

与对照组相比，心理应激组大鼠血清中细胞因子IFN-γ的含量显著高于对照组(P＜0.05)，但细胞因子IL-4的含量无变化(P＞0.05)。

Combined use of trypsin, hypertonic solution and chemical detergent is a better method to prepare acellular vascular matrix, and the vascular matrix be wellpreserved in liquid nitrogen.

液氮保存后1、2、3月扫描电镜观察与新鲜标本无明显差别。结论酶、低渗溶液、化学除垢剂联合法是制备脱细胞血管基质的较好方法，制得的脱细胞血管基质可放入液氮中保存。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。