无细胞的

MethodsThe frequency of CD4+CD25+T cells in the peripheral blood mononuclear cells from 30 cases of severe hepatitis B, 20 cases of chronic hepatitis B, 20 cases of chronic asymptomatic HBV carriers and 10 healthy controls were determined by flow cytometry and their serum HBV DNA levels were detected by Quantitative Fluorogenic PCR.

方法采用流式细胞仪检测30例重型乙型肝炎患者、20例慢性乙型肝炎患者、20例无症状乙肝病毒携带者和10例健康的外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）的水平，并应用荧光定量PCR方法测定上述研究对象血中HBV DNA滴度。

The relationship of DNA ploidy level and SP-F with age,clinical stage, ascites, histologic type,pathological grade,lymphocyte infiltration and psammoma bodies was analyzed.

作者采用流式细胞技术，对24例新鲜速冻卵巢癌组织的细胞DNA含量及S期比例(S-phasefraction，SPF)进行了定量测定，分析了它们与患者年龄、临床分期、有无腹水、组织学分类、病理分级、肿瘤组织淋巴细胞浸润及砂粒体形成之间的关系。

Results The bletilla colloid was a homogenous viscous colloid whose relative viscosity was 2324.6 mm2/s. It was easily injected through 4-F catheter and hyperattenuating under fluoroscopy, meanwhile, with good histocompatibility and hemo-compatibility, without pyrogenetic response and toxicity. In vitro, the mixture of bletilla colloid and MMC did not produce separation and suspention phenomena but released 50%of MMC at 1.8h and 100%at 3.4h. The bletilla colloid mainly embolized peripheral arteries, maintaining occlusion for 5 weeks and without formation of collateral circulation. The injuries of normal hepatic tissues were slight, without hepatic cytonecrosis.

结果 (1)白芨胶为一种均质的胶状物，有一定粘滞性，相对粘度2 324.6 mm2/s，不透X线，易经4F导管注射；(2)白芨胶具有良好的血液相容性和组织相容性，无致热原性及毒性作用；(3)白芨胶-丝裂霉素C体外混合后，无分层及悬浮现象，释放MMC 50%约需1.8小时，100%需3.4小时；(4)白芨胶主要栓塞末梢小动脉，栓塞牢靠，维持时间可达5周，不易形成侧支循环；(5)白芨胶栓塞对正常肝组织损伤轻微，未出现肝细胞坏死。

Chinensis included large parenchymatous cells at the vascular bundles, whereas no such cells were observed at the vascular bundles of A. saponaria and A. greenii .

木立芦荟、中华芦荟、易变芦荟和库拉索芦荟的维管束中有大型薄壁细胞，皂叶芦荟和绿芦荟的维管束中无大型薄壁细胞。

Telomerase activity of cavo3 cells treated with spermidine for 3 days significantly increased compared to the control group. conclusion: telomerase activity increased with the increasing of spermidine levels.

用不同浓度精脒室温处理的caov3细胞端粒酶提取液2小时后，端粒酶活性与对照组差异无显著性。caov3细胞经不同浓度精脒处理3天后的端粒酶活性与对照组相比明显增高。

Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MSCs were isolated by lymphocyte separation medium (density 1.077g/ml), MSCs were obtained by adhere culture. The surface antigen expression of MSCs was detected by flow cytometry.

将无菌条件下采集健康产妇脐带血，用相对密度为1.077g/ml的人淋巴细胞分离液分离脐血单个核细胞，利用间充质干细胞贴壁生长的特性获得MSCs，流式细胞仪检测其表面抗原标志。

Methods Using a combinatorial approach of enzymatic digestion and mechanical separation to allow isolation and culture of GER cells from P1 rat cochleae.

方法取生后1d大鼠耳蜗，利用机械分离和酶消化相结合的方法分离出纯GER细胞，分别于无血清和含10％胎牛血清的DMEM培基中进行培养，观察细胞生长特性，并对培养物进行溴脱氧尿苷、鬼笔环肽、Z01、钙视网膜蛋白及肌球蛋白VIIa免疫组化鉴定及扫描电镜观察。

The selfmade lysing solution can lyse red blood cells completely ,but not destroy leukocytes and influence the expression of surface antigens on lymphocytes, monocytes and granulocytes significantly.

自制流式细胞仪专用溶血素具有很好的红细胞溶解效果，但不破坏白细胞，且对淋巴细胞、单核细胞和粒细胞群表面抗原的表达无明显影响作用。

Result: Shengdi injection could depress the increasing of the amount of total white blood cells and neutrophils and inhibit the increasing of TNF-α, O-2, MPO caused by LPS, as well as relieve the pathologic change including Neutrophils infiltrating and mucous edema in tracheae after intravenous administration. While it did not show the effect on monocyte, and histological lesion of the lung tissue.

结果：生地注射液静脉给药可明显抑制LPS诱导的白细胞总数和中性粒细胞数目升高，对淋巴单核细胞数目无明显影响；明显抑制TNF-α，O-2，MPO水平升高；明显抑制LPS诱导的肺组织中性粒细胞浸润、气管黏膜水肿，对肺组织损伤无影响。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。