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The staining of brush cells of experimental group showed nuclear shrinkage, margination, nuclear fragmentation and formation of apoptotic bodies, and the characteristic ladder band on gel electrophoresis. NO changes were observed in control group.

实验组细胞刷片染色显示有明显的细胞核固缩、核周边染色质加深、核断裂、凋亡小体现象,细胞DNA电泳可见梯状条带的凋亡特征,而空白对照组无此现象。

The case presentation is a 70 year-old male patient who sought medical attention because of odynophagia and a right neck mass. He had a history of smoking for 50 years. Initial biopsy result was an undifferentiated carcinoma and he received a total laryngectomy combined with partial pharyngectomy and pharyngeal reconstruction and radical neck dissection. The final pathologic diagnosis is small cell carcinoma. He was then given post-operative radiotherapy. More than two years of follow-up shows no recurrence or distant metastases.

本院於2001年8月曾经治疗过1个下咽部小细胞癌之患者,他是一个菸龄50年的70岁男性,因为吞咽疼痛及右侧颈部肿块而来就诊,经切片检查结果为未分化癌,於是住院接受全喉切除并部份咽部切除与重建和右侧根除式颈部淋巴廓清术手术,手术后病理报告证实为小细胞癌,术后并接受放射治疗,追踪至今2年多,并无复发及远处转移的现象。

Results On day 7, 15, 30 after injection with exogenous VEGF, the mice in VEGF group were found more renascent blood vessels, splenic corpuscles, central arteries and peripheral lymphatic sheathes in the autotransplanted splenic tissues than the control group, and eventually formed more integral structure of white pulp, marginal zone and red pulp with much less fibrous tissue, while on day 60 there was no significant difference between VEGF group and the control group.

结果术后7、15、30 d注射VEGF组注射VEGF后较对照组的血管数量明显增多;术后60 d注射VEGF组注射VEGF后与对照组各时相点的组织学特征没有明显差别;术后7、15、30 d注射VEGF组KDR面积密度值高于对照组,术后60 d注射VEGF组 KDR面密度值与对照组比较无显著差别;术后7、15、30 d注射 KDR mRNA表达阳性细胞密度高于对照组,术后60 d注射VEGF组 KDR mRNA表达阳性细胞密度与对照组比较没有明显差异。

The cells of 2~ passage were used for proliferation kinetics studying by MTT colorimetric method.The pasagcd chondrocytes' phenotypes were as- sessed by safranine O staining and immunostaining for typeⅠ,Ⅱcollagens and aggrecan.Results For in vitro culturing of AHAC,human AB serum was superior to FBS,and the optimal concentration of human serum was 10%.But for FBS,20% was better,A similar result was got with or without the growth factors contained.

结果对于AHAC,人AB血清培养优于FBS;合适的人AB血为10%;合适的FBS浓度为20%;无血清培养细胞增殖非常缓慢;不加因子用人AB血清培养细胞梭形化明显,加用生长因子后与使用FBS在形态上一致。

When comparedwith group Ⅱ, in group Ⅲ, uterine mucosa became thick, cavum uterihad big folds, the number of glandulae uterinae increased, secreta wasseen occasionlly, blood vessels increased, cross axle of uterus becamethick.

为了检测它的吸收成分对成骨细胞增殖的作用,我们将药物吸收成分加入培养液,结果:补肾方剂药物吸收成分对成骨细胞增殖无作用(P>0.05)。

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上,本研究进一步运用免疫沉淀和Western Blot印迹方法,检测AML患者原代细胞JAK2和STAT3活化情况,并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现,11例AML患者中,有3例呈现STAT3组成型活化(STAT3阳性,下同),无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性;在此3例患者中,1例未取得缓解,另外2例虽然取得短暂缓解,但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高,而凋亡率数值较低。

"The photosynthetic and supportive cells tend to be organized linearly, but a thallus lacks such differentiated specialized structures as stem, leaves, and conducting tissue."

那些可进行光合作用和所维生的细胞呈线形结构,但原植体则无根、茎、叶的分化,无输导组织。

"The photosynthetic and supportive cells tend to be organized linearly, but a thallus lack s such differentiated specialized structures as stem, leaves, and conducting tissue."

那些可进行光合作用和所维生的细胞呈线形结构,但原植体则无根、茎、叶的分化,无输导组织。

24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

METHODS AND RESULTS: Human smooth muscle cell and fibroblast cell sheets were harvested from temperature-responsive culture dishes and transplanted into ischemic hind limbs of athymic rats.

方法和结果:人类平滑肌细胞和成纤维细胞从温度敏感的培养皿中获得移植到无胸腺鼠的缺血下肢。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。