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METHODS: Human amnion membrane derived mesenchymal stem cells were sterilely isolated and cultured. The third passages of cells (1×109/L)(0.5 mL) were transplanted by tail venous pathway to mice. Mice in the control group were injected with an equal volume of saline, whereas mice in the normal group were left intact.

无菌条件下体外分离培养人羊膜间充质干细胞,传至第3代将细胞浓度调整为1×109 L-1,经尾静脉注入0.5 mL至细胞移植组小鼠体内;对照组经尾静脉注入同体积的生理盐水;正常组小鼠不给予任何干预措施。

Results: Following treatment with siRNA 1221, the expression of exogenous CYP17 in HeLa cells was reduced by up to 50%. The CYP17 mRNA expression and androstenedione production of thecal cells were slightly, but not significantly, reduced compared to cell exposed to the non-specific siRNA.

结果:siRNA 1221处理后,HeLa细胞中外源性CYP17的表达显著降低,抑制率达50%,卵泡膜细胞中CYP17 mRNA水平和雄烯二酮产生降低,但与对照组比较,统计学上无显著差异。

Results: The Ang II levels increased significantly in the atresia follicles, either in follicular fluid or in the medium of cultured thecal cells. At the same time the renin activity levels decreased markedly. No significant change in the levels of both Ang II and renin activity was found in the medium of cultured granulosa cells.

结果:卵泡在闭锁状态下的Agn II水平在卵泡液中以及卵泡膜细胞培养液中均显著升高,肾素活性水平明显下降;颗粒细胞培养液中两项指标均无明显改变。

RESULTS: TEM displayed normal morphology of EC, its conjunction, SMC and intimal elastic lamina in control group, while in SAH group, marked pathological changes were observed, including desquamation and dystrophy of ECs, disruption of tight junction, vacuolated cytoplasm, condensation of chromatin in ECs, corrugation of IEL, and distortion of SMC.

结果: 电镜下,对照组血管壁超微结构无变化;SAH组电镜下血管内皮层结构以及内皮层细胞结构发生严重变性改变;用药治疗和预防组电镜下各组之间血管超微结构的改变无明显差异,组织变性程度明显轻于SAH组。

RESULTS: TEM displayed normal morphology of EC, its conjunction, SMC and intimal elastic lamina in control group, while in SAH group, marked pathological changes were observed, including desquamation and dystrophy of ECs, disruption of tight junction, vacuolated cytoplasm, condensation of chromatin in ECs, corrugation of IEL, and distortion of SMC. In other groups, TEM showed slight ultrastructural changes in EC, SMC and IEL.

结果: 电镜下,对照组血管壁超微结构无变化;SAH组电镜下血管内皮层结构以及内皮层细胞结构发生严重变性改变;用药治疗和预防组电镜下各组之间血管超微结构的改变无明显差异,组织变性程度明显轻于SAH组。

Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全,无细胞核碎片;瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐,结构完整;瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58%和97.25%。

The feasibility of maturing denuded buffalo oocytes in vitro was explored in this study, so as to provide a model to investigate the mechanisms of oocytes maturation.

主要探讨无卵丘水牛卵母细胞体外成熟的可行性,以便为研究卵母细胞成熟机理提供模型。

However, models of human innervated and non-innervated blood vessels have seldom been reported, and the regulating mechanism of non-innervated blood vessels is not clear yet.

故研究内皮细胞对血管的调控机制,应该区分所要研究的血管有无神经支配,而目前人体有、无神经支配的血管模型少见报道,有关无神经支配血管的调控机制的报道也为数不多。

Although IL18 gene has no cytotoxic effects in vitro, its inhibiting effects on tumorigenesis of OVHM in vivo were exerted by interdicting cell cycle and accelerating apoptosis of tumor cells.

IL18基因成功转染后,虽对体外卵巢癌细胞无直接毒性,但可能在体内借助非直接杀伤的其他途径,通过阻断细胞周期、促进肿瘤细胞凋亡等抑制体内成瘤。

Flow cytometry confirmed that DNA content was normal diploid, no induced mutation of the passaged cells.

传代后的细胞用流式细胞术测定DNA含量为正常二倍体,无诱发突变。

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此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

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