无细胞的

Insulin secretin response to glucose challenge in vitro showed the mean value of insulin in the low-glucose medium was 39.74±2.73mM, while that of high-glucose medium was 128.94±8.72mM, the SI was 3.25±0.26, that means the islets functioned well. The releasing insulin levels of the nine groups responding to 16.7mM glucose at the end of 24h and 48h: At the end of the first 24h, the controlssecretion were similar in two phases, while the G group has increasing tendency;As for oleate groups, the base secretion have no difference,while the stimulated secretion was descending;As palmic acid as concerned,both base and sitimulated secretion was descending(p＜0.01) accompany with the elevated glucose.

在培养48h后油酸组刺激后时相分泌量减少(p＜0.05)；软脂酸组两时向差别显著(p＜0.01)各组细胞在16.7mM葡萄糖刺激5min时的胰岛素mRNA表达：在培养24h及48h后，葡萄糖对照组胰岛素mRNA表达组间无明显差别(p＞0.05)；油酸组培养24h胰岛素mRNA表达组间无明显差别(p＞0.05)，培养48h后mRNA表达组间比较存在统计学差异(p＜0.05)；软脂酸组培养24h时胰岛素mRNA表达量下降(p＜0.05)，培养48h后胰岛素mRNA表达量下降明显(p＜0.01)。

Nano-Fe_3O_4 can induce DNA single-strand breaks without significancy and can not induce micronuclei formation and percentage of apoptosis. Nano-Fe_3O_4 can aggravate the effect of CdCl2 on DNA single-strand breaks and chromosome damage in HepG2 cells. They showed synergistic effects on DNA single-strand breaks after the data were statistic analyzed.

结果发现CdCl2能引起DNA断裂损伤、微核率的增加和细胞凋亡率的增加；nano-Fe_3O_4能引起DNA断裂损伤增加，但无显著意义，未引起微核率和细胞凋亡率的增加；nano-Fe_3O_4能加重CdCl2的对细胞DNA断裂损伤和染色体损伤，且二者联合作用对DNA断裂损伤的结果经统计分析认为有协同作用。

The MIP-1β mRNA expression in squama cell cancer and adenocarcinoma were significant higher than which in bronchus-alveolus cell cancer without significant difference between each other.

在肺鳞癌和腺癌的大多数癌细胞胞浆中有MIP-1β蛋白表达，在支气管肺泡细胞癌无明显表达；MIP-1β蛋白表达阳性率在腺癌和鳞癌无显著差异（69.7%比58.6%）。

The optimized DMEM, by means of a statistical optimization approach, was supplemented with some cell growth factors, such as insulin, transfferin etc.

再采用统计学正交分析方法，在初步优化的DMEM培养基中添加胰岛素、转铁蛋白等促细胞生长因子，建立了一种适于CHO C2 8细胞持续用无血清培养基———CHO C2 8 SFM。

The chlorophyll disclosing solution is an ideal dye for staining lymph nodes, which colores them quickly and not easily to precipitate, and the dyeing lymph nodes contrasted sharply with surrounding tissues, and no toxic symptoms or complication is observed after staining, the ultrastructure of the lymph nodes colored at different interval is no significant difference to the ones uncolored.

①CDS是一种优良的淋巴显色剂，染色迅速，不易沉淀，显色淋巴结与周围组织对比明显，染色持久，且染色后淋巴结细胞超微结构无明显改变，术中和术后无不良反应发生。

24H treatment group compared with 24h model group: the level of MCP-1 of the Gardenoside group decreased, but the level of MIP-1 of it is no different with the model group; the level of the MCP-1 of the Baicalin group increase and the level of MIP-1 of it all decreased; the level of MCP-1 and MIP-1 of the Concha Margatitifera Usta all decreased; the level of MCP-1 of the Cholic Acid group decreased and the level of MIP-1 is no different with the model group; the level of MCP-1 and MIP-lof the Hefang Group decreased than the model group.

在本实验中，脑缺血能够诱导P53蛋白表达，P53蛋白可能参与调控缺血后神经细胞延迟性死亡的病理生理过程，药物通过抑制细胞凋亡，从而发挥治疗作用。五、大鼠局灶性脑缺血12小时模型组缺血脑组织HSP70光密度值含量较正常组明显增高（p＜0.05）。珍珠母治疗脑缺血12小时组缺血脑组织HSP70光密度值较缺血12小时模型组无显著性差异（p＞0.05）。胆酸治疗脑缺血12小时组缺血脑组织HSP70光密度值较缺血12小时模型组无显著性差异（p＞0.05）。

However, zona hardening could occur during in vitro maturation of oocytes in a simply defined medium which reduced the fertilization rate.

采用无蛋白培养基对卵母细胞进行体外成熟培养，并防止透明带硬化，是研究影响卵母细胞成熟过程因素的基础。

In the patient group, when comparing patients subject to active infection of any virus with those under no active infection, no difference of statistic significance between them is found in terms of age, course,frequency, admitted temperature, differential leukocyte count (neutrophilic granulocyte,achroacyte and monocytes),applying glucocorticoids or not, and EDSS point value.

病例组中任一病毒近期活动性感染者和无活动性感染者相比，其年龄、病程、发作次数、入院体温、血白细胞分类计数（中性粒细胞、淋巴细胞和单核细胞）、糖皮质激素应用与否及EDSS分值间的差异均无统计学意义。

On light microscopy,there was abundant cosinophilic cytoplasm,with the tumor cells being aciniform or tubiform without significant atupia and mitosis.

光镜下瘤细胞排列成腺泡状或管状，细胞质含丰富的嗜酸性细颗粒，细胞无明显异形性和核分裂象。

Many gap junctions were observed between Interstitial cells of Cajal andsmooth muscle cells, between smooth muscle cells and smooth muscle cells in ganglionic part of HD bowel,which was absent or less in the aganglionic part.

Cx43在HD无神经节细胞肠段表达缺失提示细胞间通讯的破坏可能与HD肠功能异常有关。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。