无细胞的

The aim of this study was to establish the culture system of isolation and cultivation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

The aim of this study was to establish the culture system of isolation and cultiation of the neural stem cells from the embryonic rat brain and spinal cord. The methods of microscopic dissection, cell culture and immunofluorescence cytochemistry were used.

研究采用显微解剖、无血清细胞培养和免疫荧光细胞化学染色等实验技术，成功地建立了胚胎大鼠脑和脊髓神经干细胞的分离和培养方法。

Results Punctiform erosion, hemorrhage were observed by nake eyes in each groups. Epithelium cellular necrosis, defluxion, neutrophil being dominant in propria lamina and intact glandular were observed with microscopy. The erosion became severe with the successive stimulate of ethanol. There is no difference between distillate spirit and sodium chloride rats in Guth in 7th day, but Guth in distillate spirit rats was higher than that in sodium chloride rats in 30th day. PGE2, TGF-α contents in gastric mucosa of treated group increased significangly compared with control group. PGE2 showed no difference between each group in 7th and 30th day; TGF-α in gastric mucosa of distillate spirit and sodium chloride rats decreased after 1 month.

结果 茅台组、乙醇组、生理盐水组胃黏膜均肉眼可见程度不同的点状糜烂、出血；光镜下黏膜部分上皮坏死脱落，固有层以中性粒细胞为主炎性细胞浸润，腺体结构基本完整，病变随时间延长有所加重，Guth积分1周茅台组与生理盐水组差异无统计学意义，1月两者相比差异有统计学意义；茅台组、乙醇组胃黏膜组织内PGE2水平与生理盐水组相比明显增高，同时TGF-α表达增强；但PGE2水平各组间1周与1月相比差异无统计学意义；TGF-α表达强度在茅台组、生理盐水组1月后逐渐降低，差异有统计学意义。

On the contrary, antisense TRAP inhibited TRAP expression and downregulated endogenesis hTERT mRNA, and telomerase activity decreased.

利用Southern Blot方法检测TRAP在HeLa和293细胞中的突变情况，结果显示肿瘤细胞中TRAP基因无扩增和易位。

A standard solid viscoelastic model was employed to fit the data obtained by micropipette method.

用标准固体粘弹性模型拟合微吸管实验结果后，发现表征细胞最大变形能力的弹性系数K1 增加，而另一弹性系数K2 粘性系数μ无明显改变，说明p16使K562细胞刚性增大。

Results:①In normal extracellular solution, the non-selective cholinergic agonist acetylcholine and the muscarinic agonist muscarine both caused the increace of [Ca2+]i in separately 21 out of 25 and 14 out of 18 VHCs I, while only 3 out of 20 and 2 out of 16 VHCs I had a weak increase of [Ca2+]i if investigated in calcium free extracellular solution; nicotine could increase the [Ca2+]i in 7 out of 32 VHCsⅠonly at enough high concentration(up to 10 mmol/L) in normal extracellular solution, this [Ca~(2+)]_i increase by nicotine at high concentration could not be investigated if at calcium free solution.

结果①非选择性胆碱能受体激动剂乙酰胆碱、M型胆碱能受体激动剂毒蕈碱在正常细胞外液中均可引起大部分(21/25， 14/18)单离VHCⅠ[Ca~(2+)]_i的升高，但无钙外液中ACh、muscarine仅使少部分(3/20，2/16)VHCⅠ[Ca~(2+)]_i升高且作用减弱；正常外液中，N型受体激动剂尼古丁仅在高浓度(≥10 mmol/L)时引起部分(7/32) VHCⅠ[Ca~(2+)]_i升高，在低浓度时对胞内钙离子浓度影响不明显，无钙外液中，10 mmol/L nicotine对胞内钙离子浓度影响不明显。

When treated with different concentration of red orpiment(25～200μg/ml)for 16 hours, both NB4 and HL-60 cells showed typical apoptosis features.

浓度为12.5mg/L，培养72小时，NB4细胞表现典型的凋亡特征而HL-60细胞则无。

In addition, when the microorganism grows in sorbitol culture without Mg(superscript +), and its non-growing cells oxidate sorbitol without Mg(superscript +) sorbitol oxidation does not take place.

另外，在该菌的生长和其静息细胞转化山梨醇时，都不加入镁离子，该菌静息细胞对山梨醇无转化。

Cytological observation showed that after spraying Paclobutrazol and Unlconazole, the length of the epidermal cells of the stem became significantly shorter but not its width, its pilus epidermidis significantly shorter, the number of cells for each pilus epidermidis significantly less and the diameter of its sieve tube became significantly smaller.

细胞学观察表明，喷施多效唑和烯效唑，其茎秆表皮细胞长径显著变短，短径无显著变化；茎秆表皮毛显著变短，每个表皮毛的细胞数显著减少；茎秆筛管直径显著变小。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好；移植皮下后第4周取材可见外形呈类骺软骨样组织块，行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色，镜下观察可见大量软骨细胞及类软骨陷窝样结构，植入的海藻酸钙微球周围组织无明显炎症反应。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。