无细胞的

The toxicity and the osteogenous ability on the surface of PCHA: Cytotoxicity tests demonstrated that PCHA has excellent biocompatibility, and cells grow normally on the surface of PCHA.

CHA毒性检测和表面细胞活性的观察：PCHA浸出液与骨髓基质细胞共培养，MTT检测细胞生长曲线与空白对照组无差异，表明材料无毒性。

Both upper and lower epidermis cells are polygonal or irregular in shape in all species studied. The patterns of anticlinal walls are straight, arched or sinuous, and the raphal cuticular striastriae are occasionally observed on periclinal walls. Stomatal apparatus and secretory cells are found on the upper epidermis of several species and on the lower epidermis of all Species. The slomata apparatus mainly belong to the paracytic type and latercylic type; rarely anomocytic type occurs along with the paracytic one.

结果表明：这些植物叶片的上、下表皮细胞呈多边形或不规则形，垂周壁式样为平直、弓形或波浪状，平周壁偶有脊状条纹；少数种类上表皮还有气孔器或分泌细胞，所有种类下表皮具气孔器和分泌细胞；气孔器类型以平列型和侧列型居多，偶尔有无规则型；气孔极区呈稍角质加厚或棒状加厚，稀T形加厚。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

Whole-cellpatch-clamp technology demonstrated that VDPG (1g/L) had notsignificant effects on the delayed-rectified K~+ current, TTX-sensitive Na~+ current and high-voltage-activated Ca~(2+) current of rat dorsalroot ganglion cells. The fast transiet K~+ current of cottonbollworm dorsal DUM cells, the fast transiet K~+ current, Na~+ current andhigh-voltage-activated Ca~(2+) current of Periplaneta Americana dorsalunpaired median cells were also not significantly affected byVDPG at the same concentration. However, VDPG had significant effecton the fast transiet K~+ current of Pieris rapae. The VES had not significanteffects on the high-voltage-activated and low-voltage-activated Ca~(2+)current of rat DRG cells.

膜片钳电生理实验显示1g／L毒囊粗毒对蜚蠊DUM神经元的快瞬时钾电流、钠电流、高电压激活的钙电流，对棉铃虫快瞬时钾电流和大鼠DRG细胞延迟整流钾电流、TTX-S型钠电流、高电压激活的钙电流均无明显作用，却对菜青虫快瞬时钾电流有明显作用；电刺激粗毒对大鼠DRG细胞低电压和高电压激活的钙通道无明显作用。

The intracellular expression of viral gene and phenotypical change of transformants were examined.

结果：分离获得了可长期体外培养的SV40转化细胞克隆，已传23代以上，体外存活超过250d；DNA印迹实验显示SV40大T基因已整合至细胞染色体内，间接免疫荧光检测示多数细胞胞核内有大T基因产物的表达；转化细胞对裸鼠无致瘤性，并可正常表达角蛋白。

The same procedure was carried out 14 days later. Results The temperature of inferior segment of esophagus was significantly increased at 60min. The temperature of physic liquor was significant higher than that of perfusion. The temperature of perfusion was becoming significantly increased at 5min (P.05). The WBC, PLT, ALT, AST, Cr and CO2CP of preoperative and postoperative had no significant changes. Only lightly peritoneum dropsy and infiltrate slight phlegmasia were observed postoperatively and 14 days later. There were no significant differences among groups.

结果 在60min时食道下段温度显著升高，灌注温度明显高于抽取温度，抽取温度5min时开始升高(P.05)；各组术前和术后WBC、PLT、ALT、AST、Cr和CO2CP无明显变化，组间比较也无明显差异；术后和术后2wk腹膜均见轻度水肿、少量的炎细胞浸润；吻合口见黏膜层有小溃疡形成，表面有渗出，组间无明显差异。

MEL cells are grown in DMEM medium, induction with 2%DMSO for three days. collect cells and resuspend in DMEM medium without serum, adjust cell concentration to 5×10〓.

标准条件下培养MEL细胞，经2％DMSO诱导分化三天，收集状态良好的细胞，悬浮于无血清DMEM培养基中，调整细胞浓度为5×〓／ml。

Kinase activity assay method was used to estimate the activity of caspase-3 in the cells. RESULTS: Compared with control group and SOND group, in ASODN groups, the expression of survivin mRNA and protein were obviously weaken, apoptosis rate and caspase-3 activity apparently increased, cells growth was inhibited. In each ASODN group, the effect above-mentioned has time- and concentration-dependent manner.

结果： 转染ASODN各组OS-732细胞survivin mRNA和蛋白表达均明显弱于空白对照组、SODN组；细胞凋亡率显著增加，细胞生长相应受抑，caspase-3活性显著提高；上述效应在ASODN各组存在一定的时间浓度依赖性；而SODN各组与空白对照组间各项指标均无明显差异。

Analysis of cytosolic versus nuclear localization of HSF1 showed that in young cells HSFI was primary cytosolic, and heat shock promoted its trimerization and nuclear translocation.

在不同年龄的IMR90细胞中，无论体内或体外，HSF1激活能力与细胞年龄呈反相关，但细胞内HSF1蛋白质与其mRNA水平并无改变。

A few hematopoietic cells were seen scattered between the liver cords at 5 wk, and primitive sinusoids, which came from vitelline veins, appeared between the hepatic cords.

发育的3.5wk，部分肝干细胞及血细胞中出现VEGFA阳性反应，多数肝细胞及内皮细胞中均有VEGFC免疫反应，而造血细胞中无。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。