无细胞的

Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原细胞在卵巢中发育至早期卵母细胞时期单个或成团脱离卵巢入体腔液中，卵母细胞团细胞很快分离为单个细胞；卵母细胞在体腔液中发育成熟后进入肾管；生发泡破裂是卵母细胞成熟的标志；体腔中卵母细胞无滤泡膜，卵黄膜的形成与发育靠卵母细胞本身；卵径小于60μm的卵母细胞呈圆形，卵径大于60μm 的卵母细胞为卵圆形，形态上成熟的卵母细胞短径约115—120μm、长径约140—145μm、卵黄膜厚7—9μm。

KIR bind corresponding HLA-Ⅰmay hame four results followed:1、When inhibitory KIR bind HLA-Ⅰand there has no activatory KIR bind it,cell isn\'t dissolve because inhibitory signal access was done;2、When activatory KIR bind corresponding HLA-Ⅰand there has no inhibitory KIR bind it,form activate signal,lead cell dissolve.3、If activatory or inhibitory receptor both bind HLA-Ⅰ,both signal access is make done,when activatory signal play the main role,NK cell still actived,lead target cell dissolve.4、If activatory or inhibitory receptor both bind HLA-Ⅰ,both signal access is make done,when inhibitory signal play the main role,or both signal access don\'t play,NK cell do not make effect.this is NK cell recognice nomal tissue.

与活化型KIR受体相比，抑制型KIR受体与HLA-Ⅰ类抗原结合的亲和性更强，当抑制型和活化型KIR受体识别和结合同一HLA-Ⅰ类抗原分子时，以抑制作用为主。KIR与相应HLA-Ⅰ结合后可能产生以下4种情况：①当抑制型KIR与HLA-Ⅰ结合而无活化型KIR与HLA-Ⅰ相互作用时，因抑制信号通路启动而不产生细胞溶解；②当活化型KIR与靶细胞表面的相应HLA-Ⅰ结合同时无抑制型KIR与HLA-Ⅰ的相互作用时，形成刺激信号通路，导致靶细胞溶解。

The bulk of cells in the positive control group were dead.2 Treated with different dosages of DMF for 24h, it can be observed that green,nuclear margination and chromatin condensation were distinct in cells exposed to DMF.

研究结果 1。二甲基甲酰胺致人肝细胞凋亡形态学观察： 1不同剂量DMF染毒12h后，AO／EB染色可见阴性对照组无明显凋亡细胞，50mmol／L剂量组开始出现细胞形态接近正常，胞质为亮绿色，核出现固缩呈圆珠状，与正常细胞比较核质比变大的细胞。

The results are as follows:(1) The morphological investigation indicated that the nectaries werelacked on the main nervation, calyx and bract of cotton line 97014 comparedwith TM-1. Alveolate hollow was absent at main nervation of the mature leaf.Results of cytological analysis showed that 97014 lacked nectariferous structure.Where nectary inserted were parenchyma cells, cupped structure and brownnectariferous substance on the lower epidermis were not found.

主要研究结果如下：(1)以棉花无蜜腺品种97014与有蜜腺棉花遗传标准系TM-1为材料，进行形态学比较研究，结果表明棉花无蜜腺品种97014叶片中脉、花萼和苞叶上都缺少蜜腺，成熟叶片叶脉、花萼和苞叶上均无蜂窝状凹陷；组织与细胞切片观察发现，97014缺少蜜腺组织结构，在蜜腺着生处为排列整齐的薄壁细胞，且在下表皮看不到凹陷的组织结构和褐色的蜜腺分泌物。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2，本文针对封闭连续工艺过程的一些关键技术开展研究，包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，G2-M期延长；川芎嗪使G2-M期细胞分布增多，G2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

When inoculating, the modalities ofcells maybe round, triangle or virgulate; and began to be adhesive for 48h; andformation of colony could be formed on day 4-5; Many cells grew in clonalmanner on day 7, and the cultured cells were characterized by large spindle-shapedappearance by the time of 10 days.

刚接种时细胞形态呈圆形、三角形或棒杆状，48h 后细胞开始贴壁，4-5d 可见有集落形成，第7d 形成多个细胞克隆，第10d 细胞呈长梭形，2w 左右可达到基本融合；传代细胞呈均匀的纺锤形，基本上无悬浮细胞。

The conclusions were that the inhibitory effect of SM was the result of inhibiting the mitosis of cells and the cellular cycle be at a standstill in G2-M stage.

应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，C2-M期延长；川芎嗪使C2-M期细胞分布增多，C2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

Selective abortion stage of the stamen was primarily manifested in the stage of archesportial cell formation through anatomic observation for male sterile flower and male fertile flower. There was no archesportial cell development or anther production and was a male sterile line without anther.

通过对不育系和可育系小花的细胞及形态解剖学观察，初步确定万寿菊雄蕊选择性败育的时期，为花药受阻于花药原基细胞分化期，无孢原细胞形成和药室分化，属于无花粉囊型不育系。

AIF ex- pression in ischemia region of cortex of parietal lobe and nerve cell apoptosis degree in sham operation group, MCAO 90 min and reperfusion 0, 3, 6, 12, 24, 48 and 72 h were observed by TUNEL and immunohistochemistry technique.

除缺血90min凋亡细胞数量与假手术组比较无统计学意义外，其余各指标与假手术组相比均有统计学意义（P〈0.01），除6与12h以及24与48hAIF含量的差异无统计学意义，24与48h凋亡细胞数量的差异无统计学意义外，其余各组间差异均有统计学意义（P〈0.05）。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。