无细胞的

Amitosis and asymmetric division play an important role in PC12 cell pleomorphism. The asymmetric division possesses important significance in tumorigenesis and differentiation.

无丝分裂和不对称分裂是PC12细胞多形性形成的主要原因，肿瘤细胞无丝分裂及其不对称性对肿瘤发生及肿瘤细胞的分化具有重要意义。

The results of character evolution history reconstruction shows the SC in PT and AD clade might be a homoplasy character; also, the SC in PT clade might have multiple origins. SC in Pteridaceae has some special characteristics:(1) the cell long axes usually run parallel to the lateral veins;(2) Most of Pteris and Adianum species have VSC only in lower epidermis;(3) Species have ISC also have VSC.(4) Presence of spicular cells is highly correlated with the long veinal epidermal cells. Species have type III silica deposition type usually have longer and narrower veinal epidermal cell compared with ordinary epidermal cells; Species have type IV silica deposition type also have long epidermal cells, but not significantly different from ordinary epidermal cells.

本实验中亦发现凤尾蕨科之矽异形细胞具有几项特色：(1)矽异形细胞之长轴多与叶片侧脉方向平行(2)发现多数铁线蕨属以及凤尾蕨属植物仅下表皮具有脉上矽异形细胞；(3)具有脉间矽异形细胞的种类会同时具有脉上矽异形细胞；(4)长条形的侧脉上表皮细胞与矽异形细胞具有相关性，仅具脉上矽异形细胞的分类群之脉上表皮细胞均较一般表皮细胞细长；具有脉间矽异形细胞之物种之脉上表皮细胞亦为长条形但形态与一般表皮细胞常无明显形态差异。

The cells were incubated in RPMI 1640 without or with different contents of glutamine and with 10%fetal calf sera, at 37℃,5% CO2 and saturation humidity for 4 days.The initial living cell density was 5×108/L. After 4 days incubation,the cells were counted,collected, smeard and stained with Wright-Giemsa, DNA, POX, NAE and NaF inhibition,gulping ink and NBT etc. cytochemical technology. The stained cells were investigated under oil immersion lens.

从18例未经治疗的APL病人外周血中提取APL原代细胞，在无Gln的RPMI 1640培养液中补加10%胎牛血清，以活细胞密度5×108/L接种细胞，在37 ℃， 5%CO2和饱和湿度下培养4 d，计数活细胞，并收集细胞行Wright-Giemsa、DNA、POX、NAE及NaF抑制试验、墨汁吞噬试验和NBT还原试验等细胞化学染色，于油镜下观察。

The culture medium is composed of human keratinocyte serum free culture medium +2.5 mg/L bovine pituitary lixivium +5 μg/L epidermal growth factor+438 mg/L glutamine, glutamine can promote the growth of the keratinocyte.

采用改良的无血清培养技术进行人体角质形成细胞的培养，培养基为人角质形成细胞无血清培养基+2.5 mg/L牛垂体浸出液+5 μg/L表皮生长因子+438 mg/L谷氨酰胺，其中谷氨酰胺能促进角质形成细胞的生长。

RESULT: The activity and drinking of mice who had proceeded bone marrow transplantation after 60Co total body homogeneous radiation was no significantly different with normal mice, but their pelage became grey, blood samples detection were also same with normal mice after five weeks of bone marrow transplantation, the proportion of GFP positive cells in bone marrow cells achieved 59.62±13.21% proved by laser confocal microscopy, all these evidence proved the bone marrow reconstitution had been finished in the recipients.

结果： 60Co全身均匀照射后进行骨髓细胞移植的骨髓移植组小鼠的活动与饮食与正常小鼠无明显差别，只是毛发渐变灰白，五周后其血常规与正常鼠相较无明显差异，流式细胞仪检测显示其骨髓细胞中GFP阳性细胞的比例也达到59.62±13.21%，提示骨髓重建成功。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

The two cells that produced by the amicronucleate cell were still without micronucleus.

在整个接合过程中，无小核细胞的大核始终没有发生可观察到的形态学变化，它最终分裂成的二个子细胞仍为无小核细胞。

The ultrastructure characters of pollen grain in Brassica napus are:1There are vacuoles in cytoplasm but no starch grain in uniceelluar pollengrain.2The veget- ative nucleus of bicellular pollen grain is generally spheroidal;while the generative cell is spinde-shaped,with a big nucleus,thin layer of cytoplasmand few cell orgens,no cell wall.3In three-celled pollen grain the sperm cell is separated from the cytoplasm of vegetative cell by two layers of plasmic membrances.

Brassica napus的花粉粒，在不同发育时期超微结构的特征如下：1单胞花粉粒有一个球形的细胞核和明显的核仁，细胞质内出现液泡，缺少典型的淀粉粒。2双胞花粉粒的营养核多为球形。生殖细胞纺锤形，无细胞壁，细胞核比例大，细胞质呈薄层，细胞器少。3三胞花粉粒有一个裂片状的营养核和两个纺锤形的精细胞，精细胞无壁，以两层质膜与营养细胞的细胞质相隔。

In the PbⅠ stage oocyte, perivitelline enlarged further, the cumulus cells expanded and the cell junctions disappeared. On the membrane of oocyte near the first polar body, a no microvillus area was observed, where probobly was the position for the second polar body extruding.

在PbⅠ阶段的卵母细胞内，卵周隙进一步扩大，卵丘细胞扩散，无细胞间连接存在，卵母细胞表面靠近第一极体的部位存在无微绒毛的区域，可能是第二极体释放的部位。

Methods Chromosome, HE stainings and autoradiography were made for the cornea of human and monkey.

目的 探讨灵长目动物角膜内皮细胞的再生能力及方式方法对猴及人角膜做染色体染色、HE染色及放射自显影结果成年创伤活体猴角膜内皮细胞有再生能力成年及幼年灵长目动物角膜内皮细胞在生理和病理条件下的再生方式均为无丝分裂结论灵长目动物角膜内皮细胞在一定条件下有再生能力，其再生方式为无丝分裂

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。