无细胞的

Aim:To observe the amitosis and asymmetric division in subcultured PC12 cells, and to explore the role of amitosis and asymmetric division in tumorigenesis, tumor cell pleomorphism and differentiation.

目的 ：观察传代培养的PC12细胞的无丝分裂和不对称分裂，探讨其在肿瘤发生、肿瘤细胞多形性和细胞分化中的意义。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜，含类似上皮层和固有层，但细胞层次不清；胶原膜上可见口腔黏膜上皮细胞黏附生长，并形成复层上皮样组织，但无成熟的桥粒及基底膜样结构；以Alloderm作为支架材料的构建物，出现正常上皮形态，含半桥粒及细胞指状突起等超微结构。

When seedlings were cold-acclimated at 4℃ for 7d, the ATPase activities on the PM and tonoplast were higher than those of the seedlings treated at 22℃.

标志ATPase活性反应的磷酸铅沉淀物主要分布在叶片栅栏细胞和海绵细胞的质膜和液泡膜上，ATPase在这两类细胞中的分布及活性无明显差异。

The electron-dense lead phosphate deposits, an indication of ATPase activity, mainly distributed on the plasma membrane and tonoplast in the mesophyll cells of palisade and spongy tissue, in which there were no obvious difference of ATPase distribution and activities.

结果表明：标志ATP酶活性反应的磷酸铅沉淀物主要分布在叶片栅栏细胞和海绵细胞的质膜和液泡膜上，ATP酶在这两类细胞中的分布及活性无明显差异。

During the development of dental germ, enamel matrix proteins secreted by epithelial root sheath could induce dental follicle cells to differentiate towards cementoblast and form acellular cementum finally on the surface of tooth root. Many researches of domestic and oversea scholars suggest that enamel matrix proteins could effectively induce periodontal regeneration, and the structure and function of this regenerated tissue are similar to healthy periodontal tissue. Moreover, the regeneration process is nearly the same to that of embryonic development of periodontal tissue.

自发现釉基质蛋白可诱使牙囊细胞向成牙骨质细胞方向分化并在根表面形成无细胞性牙骨质以来，国内外学者做了大量研究，结果表明釉基质蛋白能有效诱导牙周组织的再生，且这种再生组织的结构和功能更接近于健康的牙周组织，并且再生过程也与牙周组织的胚胎发育过程类似。

On the other hand, cells can only undergo differentiation when they were cultured without FBS, which enhances the rate of chondrogenesis by increasing the differentiation efficiency.

相对的，在无胎牛血清的环境下，细胞就可以专一地进行细胞分化，而使其分化成软骨细胞的速度加快，提高分化的效率。

2TNF-α in the concentration of 10-3-10u/ml significantly inhibited TSH-induced cAMP release by both normal and hyperfunctioning thyroid cells.

2在10~(-3)～10U/ml的浓度区间，TNF-α显著抑制TSH对正常和甲亢甲状腺细胞生成cAMP的刺激效应，10~3U/ml的TNF-α仅抑制TSH对正常甲状腺细胞的刺激作用，对甲亢甲状腺细胞无明显影响。

Effects of sinusoidally varying magnetic fields with one frequency of 2HZ and static magnetic fields with different strength of 10GS、200GS and 560GS on proliferation of osteoblasts had been observed. It had been found that PEMFs promoted proliferation of osteoblasts, static magnetic fields with intensities of 10GS and 200GS did not affect its proliferation whereas that of 560GS inhibited the proliferation.

通过观察频率为2HZ的正弦波电磁场及强度分别为10GS、200GS、560GS的静磁场对成骨细胞增殖的影响，结果显示：脉冲电磁场能显著促进成骨细胞的增殖；强度为10GS、200GS的静磁场对成骨细胞的增殖无明显影响，而强度为560GS的静磁场抑制成骨细胞的增殖。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。