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Those proteins are the cell special proteins of neuron, glia, photoreceptor, bipolar cell, amacrine cell, horizontal cell and the ganglion cell of retina respectively.

并且可表达视网膜的终末分化细胞的标志,包括:感光细胞标志Rhodopsin、双极细胞标志PKC、无长突细胞标志Syntaxin、水平细胞和无长突细胞标志Calbindin和视网膜节细胞标志Thy-1。

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果:(1)在无血清条件下单独培养的DRG,背根神经节的轴突数目众多,外形纤细弯曲,不成束,并且施万细胞和成纤维细胞稀少,所以可以排除两者对轴突生长的影响,为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件;(2)在无血清条件下DRG和变性坐骨神经段联合培养,①先单独培养DRG,4天后待神经元轴突长出,再与坐骨神经段联合培养,观察到神经元的轴突数目减少,外形挺直,部分轴突之间相互粘附成束;②变性坐骨神经段和DRG同时联合培养,神经元的轴突数目明显减少,外形粗壮,轴突之间相互粘附成束;(3)有血清条件下单独培养DRG,轴突数目较多,外形挺直,长短不一,部分神经元的轴突之间相互粘附,施万细胞和成纤维细胞数目众多,观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Single-celled or noncellular spherical or spiral or rod-shaped organisms lacking chlorophyll that reproduce by fission; important as pathogens and for biochemical properties; taxonomy is difficult; often considered plants.

单细胞或无细胞的球状或螺旋状或棒状的缺乏叶绿素的有机体;通过分裂繁殖;和病原体一样对生物化学很重要;很难分类;通常被认为是植物。

The results showed that,on first day induced by mIL-2+B16,mIL-2+B16PELs had no cytotoxicity to B16 target cells,no expression ofperforin and granzymeA genes,no granules in PELs cytoplasm.

结果显示:1.mIL-2〓细胞诱导一天时,取得的PEL用Rt-PCR检测,无粒酶A与穿孔素基因表达、无细胞毒活性、其胞浆中也无杀伤颗粒存在。

Specimens were harvested at the 2nd and 4th week after injection, and stained with HE and toluidine blue.

在注入无载体的软骨细胞悬液组,部分动物的皮下注射点有少量软骨组织形成,而在注入无细胞的藻酸钙载体组则未见软骨形成。

The differentiation states of costae indicates the ways they absorb and transport water, and their ability adapting to the dry conditions, which was elucidated by the following examples: Brachythecium plumosum, because of its thin cell-walls in the costae and the absence of hydrome, assistant and steroid cells, can absorb water and nutrition under shady and moist conditions; Plagiomnium rostratum, though often grows in shady and wet condition, has hydrome and steroid cells, a feature similar to those of xeric mosses, which endows it to grow in the conditions with periodical drought stress during its life cycle; Hygrohypnum luridum is characterized by its slender leaves with only one layer of cells, the thin cell-walls, the absence of filaments on the leaf surface, and the fewer layer cells in the costae, the absence of hydrome and steroid cells. These features make it adaptable to aquatic environments. For Pogonatum inflexum and Atrichum undulatum, their ventral surfaces covered with lamella, while for Racomitrium japonicum, Thuidium cymbifolium, Macromitrium ferriei, Diphyscium fulvifolium, Barbula unguiculata and Ceratodon purpureus, their leaves are strongly mammillose or papillos. Such appendiculate structures made them adaptable to thy conditions.

例如,荫湿生环境下的羽枝青藓Brachythecium plumosum,其中肋细胞胞壁较薄,无导水主细胞和副细胞的分化,也没有厚壁细胞分化,能够在阴湿环境下吸收水分和养分;钝叶匍灯藓Plagiomnium rostratum具有与旱生藓类植物相似的中肋结构,叶片较厚,中肋具导水主细胞,中肋背面具厚壁细胞,这些特点使该种藓类植物能够分布于间隙性干旱胁迫的环境中;水灰藓Hygrohypnum luridum叶片纤细柔弱,仅1层细胞,细胞胞壁薄,叶表无附属结构,中肋细胞层数少,无导水主细胞分化,也没有厚壁细胞,这些特点使得水灰藓'能够生长在水生环境中;东亚小金发藓Pogonatum inflerum和波叶仙鹤藓Atrichum undulatum的叶腹面覆盖着栉片,东亚砂藓Racomitrium japonicum、大羽藓Thuidium cymbifolium、福氏蓑藓Macromitrium ferriei、东亚短颈藓Diphyscium fuhifolium、扭口藓Barbula unguiculata和角齿藓Ceratodon purpureus的叶片表面有乳头状突起或疣状物,这些附属结构使它们能够适应于旱生的环境中。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or were of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes, with micropapillae floating freely in spongy spaces and separated by fibrous septa.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列,癌巢与周围间质形成明显的空隙,微乳头缺乏纤维血管轴心,每个微乳头细胞团和周边的纤维组织均存在无细胞的间隙样结构;瘤细胞CerbB-2、CgA和EMA。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示:1BP刺激细胞可促进c-Jun活化,并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性,均可明显抑制BP诱导的c-Jun活化,但阻断p38活性对BP引起的c-Jun活化无明显影响,提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化,并降低磷酸化ERK的表达水平,但对磷酸化JNK的表达水平无明显影响,说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加,并同时诱导Akt和ERK的磷酸化水平的升高,表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况,发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加,提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化,降低磷酸化Rb以及E2F1蛋白表达水平,表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高,可见c-Jun也参与调控BP诱导的p53/p21通路活化。

Comparing with control group, CD4~+ T cells of AIDS patient were markedly depleted in gastro-mucosal tissues(P<0.01), but in presymptomatic patients CD4~+ T cells were not significantly different. CD3~+ T cells, CD8~+ T cells and NK. cells were notably increased (P.01), and they severely infiltrated glands and epitheliums in gastro-mucosal tissues. And the quantity and distribution of CD20~+ B cells between patients and control group were not obviously different.

HIV感染无症状者和AIDS患者胃黏膜活检组织中CD3~+T细胞、CD4~+T细胞、CD8~+T细胞、NK细胞、B细胞的数量及分布:与非HIV感染对照组相比,(1)AIDS患者胃黏膜内CD4~+T细胞显著减少(P<0.01),而无症状者与对照组无显著差异(P>0.05);(2)胃黏膜内CD3~+T细胞、CD8~+T细胞、NK细胞(CD57~+)显著增加(P<0.01),并呈现较明显普遍性噬胃黏膜上皮和腺体现象;(3)各组胃黏膜内CD20~+B淋巴细胞的数量分布变化不明显。3。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中,待细胞生长融合后在培养板上划线,造成无细胞的裸露区,分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液,每6h观察记录移行至裸露区的细胞。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。