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The invention discloses a connecting city white duck blastodisk fiber cell system with connecting city white duck embryo as material with preservation number at CGMCC No.1877 in cellular biology domain, which is characterized by the following: the fiber cell does not possess epithelial cell with high purity; the quality of freezing cell is stable; the active ratio can reach between 93.5% and 96.8%; the passage growth is stable and fit for big scale culture.

本发明利用连城白鸭胚胎作为材料,进行初代培养、传代培养及细胞冻存等研究。最终获得高活率、高纯度的连城白鸭胚成纤维细胞系,其保藏编号为CGMCC No.1877属于细胞生物学领域。本发明培养的成纤维细胞无上皮细胞等,细胞纯度高;冻存后细胞质量稳定,活率可达到并维持在93.5%~96.8%之间,传代生长稳定,适合大规模培养。

It included enzyme unhairing,sodium hydroxide based fibre opening and pickle and basification-free chrome tanning process.

介绍了一种三步铬鞣山羊面革的加工方法,即:酶脱毛,NaOH打开皮纤维和无浸酸、无提碱铬鞣。

After 14 weeks of operation, the structure of epineurium was in integrity and there was no obvious difference in perineurium and endonurium between two groups.

结果 术后 2周,A组炎性反应稍重于 B组;至 4周时两组的炎性反应程度相似,近端少许胶原纤维增生;8周时两组的炎性反应基本停止,胶原纤维增生稍明显;14周时两组神经外膜构成完整,束膜、内膜结构无明显差异。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

Images captured by it are clear and clean without noisy signals and back scales, can satisfy image recognizing technique with computer.

用其采集的纤维图像鳞片清晰,边缘清楚,无背景噪音和背面鳞片干扰,能够满足毛绒类纤维计算机图像分析技术的基本要求。

Here we present a case of 21 year-old male who suffered from recurrent swelling in left side of mandible which had received surgery for ossifying fibroma in another hospital when he was 19 years old. Obvious facial asymmetry was noted but no sensory dysfunctions were detected. The lesion showed well-demarcated, multilocular radiolucency but radiopacity centrally. We applied segmental resection via extra-oral approach and reconstructed with anterior iliac crest bone graft. After removal of MMF in six week, the wound healed well and joint function was good without malocclusion. After follow-up of one year, no recurrences were noted.

本病例为-21岁之男性,在19岁时因为左下颚骨角区肿大在某医院接受外科手术处理,术后病理报告为骨化纤维瘤,因为复发的肿胀而来本院寻求治疗,临床检查并无疼痛及下唇区感觉异常的问题,放射线影像为周界清晰,多腔室放射透过性合并中央放射不透过性之不规则小块,手术方式为从口外颚下切线,做下颚骨片段式切除,并以肠骨脊海绵骨移植修补骨缺损加以颚间固定六周,术后伤口恢复及愈合良好,颚骨关系稳定无咬合异常的情况,无张口受限的问题。

Preferential small myelinated and unmyelinated fiber loss correlated to the cases of severe pain.

外周小的有髓纤维和无髓纤维缺失与严重疼痛的病例有关。

The latter group showed marked pain, in particular, severe mechanical hyperalgesia, and predominantly small myelinated and unmyelinated fiber loss.

后一组突出表现为疼痛,特别是严重的机械性痛觉过敏,主要是小的有髓纤维和无髓纤维缺失。

Fruit drupaceous, exocarp leathery, thin; mesocarp carnose, spongy, or with numerous radial soft fibers, sometimes fibers nearly absent when dry; endocarp hard, woody, rarely thin, outer face reticulate-lacunose.

果为核果,外果皮革质,薄;中果皮肉质,海绵状,或者具许多辐射状柔软的纤维,有时纤维近无在干燥时的;内果皮硬,木,很少薄,外面网状多洼。

Pseudoacacia and Caragana were significantly (P.05) better than A. fruticosa in pH, lactate content and ammonia-N/total-N ratio, so that their fermentation quality was better. In addition, they were high in crude protein and low in neutral detergent fiber and acid detergent fiber, showing that they were abundant in nutritive components. A. fruticosa was the inferior silage in every evaluation in the experiment. The mixed silage of R. pseudoacacia and Caragana was the best, and the mixed silage with A. fruticosa was not recommendable. There were no obvious changes in the time before and after silaging in nutritive value of these three kinds of plants, it showed that the silage technology was good.

结果表明:刺槐、柠条青贮伺料在pH值、乳酸含量、氨态氮/总氮比值方面均显著地(P.05)好于紫穗槐处理,发酵品质良好,且粗蛋白含量高,中性洗涤纤维和酸性洗涤纤维含量低,营养价值高;而紫穗槐青贮品质在各项评定中均较差;在混合青贮中刺槐和柠条青贮混合品质最佳,与紫穗槐混合的两种混合饲料品质较差;刺槐、柠条、紫穗槐在青贮前后营养品质均无明显降低,青贮饲料品质等级均为良好,青贮效果较优。

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