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The average molecular mass of polysaccharide is 8.6×10^4 u. Polysaccharide is composed of glucose, arabinose and uronic acid. There is no absorption peak of protein and nucleic acid at 280 and 260 nm.

大叶金花草多糖平均分子质量为8.6×10^4 u,由阿拉伯糖、葡萄糖和糖醛酸组成,在260和280nm处有多糖无核酸和蛋白质的吸收峰。

The influencing factors of femoral necrosis included the degree of healing activity, the dosage summation of corticosteroids, and length of ictus therapy. The influencing factors of bone density included age, dosage summation, and length of ictus therapy. The influencing factors of the bone fabric and flexibility included the use and length of ictus therapy. Statistics showed that serum IgG was not related with avascular necrosis of femoral head and osteoporosis.

股骨头缺血性坏死的影响因素有后期活动量、糖皮质激素总量和糖皮质激素冲击疗法的持续天数;骨密度的影响因素有年龄、冲击疗法和糖皮质激素总量;骨结构和骨弹性的影响因素有冲击疗法、冲击疗法持续的天数;血清抗体IgG与患者康复期股骨头缺血性坏死、骨质疏松在统计学上无联系。

For instance, because the job is busy,Wu Man of Chinese Shanghai girl sees a lot of office worker, have a meal in cafeteria everyday almost, then bud commute in the white-collar need the subway of the ground of classics and office building and other places sell organic the commercial pattern of only course, wu Man hopes to hand the idea of healthy diet to modern with this; The layout case name of the bavin of old Da · that comes from Kampuchea is " your business affairs solution ", its business plan is the blame productiveness orgnaization that is Kampuchea place, or it is office of financial insolvency big accountant the small company of high cost, provide petty gain, the client is custom-built the accountant solution of type; And year only Philippine girl of 23 years old Kailuolin Kua, criterion the Philippine food culture with aspire pure and healthy general pushs to the world: She will begin will Philippine have a kind of common orange kind the fruit applies in food, dressing and beverage widely, poineering idea is this kind of fruit treatment is become without candy, need not compress, the inspissation that can save 6 months juice.

比如,中国上海女孩巫曼看到很多上班族由于工作繁忙,几乎天天都在餐馆里吃饭,于是萌发了在白领上下班必经之地的地铁和写字楼等地出售有机净菜的商业模式,巫曼希望以此给现代人传递健康饮食的概念;来自柬埔寨的陈妲·柴的企划案名为"你的商务解决方案",其商业计划是为柬埔寨当地的非赢利性机构,或是无力支付大会计师事务所昂贵费用的小企业,提供便宜的、客户定制式的会计解决方案;而年仅23岁的菲律宾女孩凯洛林·库阿,则立志将纯净健康的菲律宾饮食文化推向世界:她将着手将菲律宾有一种常见的柑橘类水果广泛地应用于食物、调味品和饮料中,创业想法是将这种水果加工成无糖、无需压榨、可以保存6个月的浓缩汁。

This paper disscussed the permeating of sugar and quality of candied apple with several pretreating methords.

在低糖无硫苹果脯生产的糖制和烘烤过程中,采用高真空技术可显著地缩短加工时间,并有效地使低糖无硫果脯保持原色、原味,且饱满、透明。

Low sugar and non-sulfur candied apple processing was studied by vaccum techniquing.

在低糖无硫苹果脯生产的糖制和烘烤过程中,采用高真空技术可显著地缩短加工时间,并有效地使低糖无硫果脯保持原色、原味,且饱满、透明。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P<0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P>0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6%final gel concentration and there were 15~19 bands identified on this condition.

同功酶电泳图谱显示,1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著,均以LDH-5为主,但1℃组LDH-1显著高于室温组(P<0.05),推测黑龙江林蛙为适应低温低氧环境,增强LDH-1酶活性以较好的利用乳酸,避免因大量乳酸形成造成肝脏疲劳;而1℃组肝脏G6PDH酶活性无显著变化,进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调,维持机体代谢的稳定;1℃组和室温组EST总酶比活力无显著变化,但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P<0.05),推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性,增加肝脏酯类物质代谢,为机体提供维持生命必需的最低能量。

Circular dichroism spectroscopy measurements also reveal that the ordered structure content of α-helical and β-structure of ovalbumin decrease, and the unfolded random structure is enhanced with increasing pressure; While the existence of dextran sulphate to the native ovalbumin, there are no apparent change on the secondary structure of ovalbumin; It seems same to glycinin.

圆二色性光谱分析表明,经超高压处理后,鸡蛋清蛋白分子中规则的二级结构减少,而部分规则的二级结构和无规卷曲含量增加。加入硫酸右旋糖苷的鸡蛋清蛋白溶液经超高压处理后其二级结构中各部分的含量变化不大:对于大豆豆球蛋白,加入硫酸右旋糖苷后,大豆豆球蛋白分子经超高压处理后其有序结构的减少及无规卷曲的增加都相对减缓。

Sugar and protein content in the haemolymph after fed with transgenic rice Total sugar content in the haemolymph of SSB fed with KMD1 was significantly lower than that of the control, while trehalase activity was significantly higher than that of the control, but there were no significant differences on trehalose concentration and glucose concentration between the treatment and the control. However, there were no significant differences on all the four components mentioned above between SSB fed on MSB and the control.

四、取食转基因抗虫水稻后二化螟幼虫血淋巴糖代谢和蛋白质浓度的变化五龄二化螟幼虫取食KMD1后血淋巴总糖含量显著低于取食秀水11对照的,海藻糖酶活性显著高于对照,而海藻糖含量和葡萄糖含量与对照组相比都无显著差异;取食MSB后二化螟血淋巴总糖含量、海藻糖含量、葡萄糖含量以及海藻糖酶活性与取食明恢86的对照相比,均差异不显著。

In the paper,the fresh,withered,rocked and fermented leaf fixed by microwave and steam were studied.The results showed that the content of pol yphenols of microwave samples was higher than those of steam samples;and the con tent of amino acids in steam samples was higher than those of microwave samples .There was a slight higher content of soluble sugars in microwave samples compa red with steam samples. The content of chlorophyll of microwave samples was hig her than steaming samples,and there was a small difference of caffeine content b etween microwave and steam samples.There was a little higher content of theafla vins in microwave samples than steam samples.The content of thearubigins in steam samples was higher than microwave samples,and there was no differe nt theabrown between microwave and steam samples.

摘 要:对鲜叶、萎凋叶、做青叶和发酵叶进行了微波和蒸青固样研究,结果表明:微波样的多酚类化合物保留量均高于蒸青样,氨基酸保留量则相反,可溶性糖的保留量微波样稍高于蒸青样,叶绿素的保留量微波样亦稍高于蒸青样,咖啡碱的保留量微波样与蒸青样间差异较小,发酵叶茶黄素的保留量微波样稍高于蒸青样,发酵叶茶红素的保留量微波样高于蒸青样,发酵叶茶褐的保留量二者间无差异。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行:1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上,37℃、5%CO2的条件下培养,3~5天后洗下菌苔,经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原,经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔,采用琼脂糖双向扩散试验检测,两种抗体血清效价均为1:32;饱和硫酸铵法提取抗体,SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒;分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针,两种抗体的最适标记量均为30μg/ml;选择适当孔径的微孔滤膜为载体包被两种抗体,NC膜包被浓度均为5mg/ml。

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