无精的

More eternal than Rome, more splendorous than Nineveh. The very navel of the world to which, like a blind and faltering idiot, one crawls back on hands and knees. And like a cork that has drifted to the dead center of the ocean, one floats here in the scum and wrack of the seas, listless, hopeless, heedless even of a passing Columbus. The cradles of civilization are the putrid sinks of the world, the charnel house to which the stinking wombs confide their bloody packages of flesh and bone.

它比罗马更久远，比尼尼微更壮观，它是世界的肚脐，人像一只漂到大洋中死一般寂静的软木塞，独自漂浮在这儿，在海洋的渣滓和船只残骸之中，无精打彩、毫无希望，连路过的哥伦布也不去注意他，文明的摇篮也就是扔全世界的腐肉的污水坑，就是尸体存放所，发臭的子宫把骨肉的血污包裹放在里面。

First observation of the essential oil of UVA (320-400nm) absorption, taking nine more before the absorption of essential oils and nine of the past record or doubt further analysis of essential oils, UVA nine large oil absorption of the former are: cinnamon, pheasant pepper, sweet orange, lemon, basil, grapefruit, bluegrass rock, roses, dill, 9 kinds of essential oils may be phototoxic as follows: orange blossom, bergamot, fennel, lemon grass, red mandarin, citronella oil, ginger oil, Australia sandalwood oil, lime; E.

首先观察精油的UVA(320-400nm)吸收度，取前九名有较大吸收之精油及九种过往记载或存疑之精油进一步分析，UVA较大吸收度之前九名精油分别为：肉桂、山鸡椒、甜橙、柠檬、罗勒、葡萄柚、岩兰草、玫瑰、莳萝，九种可能光毒精油为：橙花、佛手柑、茴香、柠檬草、红柑、香茅油、姜油、澳洲檀香油、莱姆；大肠杆菌抑菌圈变化中，柠檬、红柑及葡萄柚精油有明显的扩大现象；pUC119 DNA电泳结果，不同精油於电泳模式中，随UVA照光能量增加产生不等断裂情形，除玫瑰精油无影响，其余精油在UVA照光能量增加下皆有不等的DNA断裂情形发生，须再进一步试验。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状，由精子群、精荚基质及初级精荚壁和次级精荚壁组成，初级精荚壁与精荚基质是相连续的，它们之间无明显界限，两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成，但后者不含精子，后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物，可能与形成精子细胞核中的泡状带和膜状带有关，在交配后的雌虾，其头胸部腹面上粘着的精荚中，其基质内已不见絮状和同心圆泡，仅见稀疏的纤丝。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术，一种利用高压喷射技术，在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素，以雾态的形式定点、定量、定层、定位，以扇状的形直接、准确的到达皮肤中胎层，20分钟起效，2小时见效，48小时显效，无创无副作用，可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓，7天之内消除脂肪，重塑苗条身材，无恢复期，对棉布轮廓雕塑精准度可控制的毫米之间，不光是解决了衰老问题，可使原本漂亮的脸更加精致，维持之久。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

去 美容美发保健品 IM互动商圈，与商家群聊发现更多商机。共有24条同类&美体器材&信息，请通过分页查看 12 3 4 5 无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术，一种利用高压喷射技术，在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素，以雾态的形式定点、定量、定层、定位，以扇状的形直接、准确的到达皮肤中胎层，20分钟起效，2小时见效，48小时显效，无创无副作用，可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓，7天之内消除脂肪，重塑苗条身材，无恢复期，对棉布轮廓雕塑精准度可控制的毫米之间，不光是解决了衰老问题，可使原本漂亮的脸更加精致，维持之久。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Since the plant has, the Company has always adhered to the "market"-oriented in order to "talent" as the fundamental to the "clients" for God's business philosophy of "my people have no, I have new people, new people my spirit" production principles, all the staff together, continue to adhere to the spirit of customer first, to high-quality, high credibility of the new and old customers return for the roller sports in our country to flourish and contribute to, and promote continuous healthy and stable development of the company, in the near future,"Speed flying fish" will become the field of sports a shining pearl.

自建厂以来，本公司始终坚持以&市场&为导向，以&人才&为根本，以&顾客&为上帝的经营理念，采取&人无我有，人有我新，人新我精&的生产方针，全体员工团结一致，继续坚持客户至上精神，以高品质、高信誉回报新老客户，为我国的轮滑体育事业蓬勃发展贡献力量，促进公司持续健康稳定发展，在不久的将来，&飞鱼速滑&必将成为体育事业领域中一颗璀璨的明珠。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。