无精

Results showed that TDI induced toxic action on spermatogenic cell,while the chondriosome was its target within the length and concentrations.

结果表明：在本实验染毒时间和染毒剂量范围内，TD I对雄性的生殖毒性主要表现为对各级生精细胞均有一定的毒性作用，作用的靶点是细胞内的线粒体；TD I通过影响各种酶的活性，干扰了睾丸组织的有氧代谢和无氧供能，抑制了细胞对能量的利用，损伤了各级生精细胞，导致生精上皮不可逆的损害，从而造成了其对雄性生殖系统的损伤；TD I对雄性生殖细胞的核酸代谢以及DNA合成有一定影响。

No lock, thrusting, single intromission, and single ejaculation) of Dewsbury's system and No.

属于Dewsbury提出的交配模式系统中的第11种（无锁结，有抽动，单次插入多次射精）和第12种（无锁结，有抽动，单次插入，单次射精）。

First observation of the essential oil of UVA (320-400nm) absorption, taking nine more before the absorption of essential oils and nine of the past record or doubt further analysis of essential oils, UVA nine large oil absorption of the former are: cinnamon, pheasant pepper, sweet orange, lemon, basil, grapefruit, bluegrass rock, roses, dill, 9 kinds of essential oils may be phototoxic as follows: orange blossom, bergamot, fennel, lemon grass, red mandarin, citronella oil, ginger oil, Australia sandalwood oil, lime; E.

首先观察精油的UVA(320-400nm)吸收度，取前九名有较大吸收之精油及九种过往记载或存疑之精油进一步分析，UVA较大吸收度之前九名精油分别为：肉桂、山鸡椒、甜橙、柠檬、罗勒、葡萄柚、岩兰草、玫瑰、莳萝，九种可能光毒精油为：橙花、佛手柑、茴香、柠檬草、红柑、香茅油、姜油、澳洲檀香油、莱姆；大肠杆菌抑菌圈变化中，柠檬、红柑及葡萄柚精油有明显的扩大现象；pUC119 DNA电泳结果，不同精油於电泳模式中，随UVA照光能量增加产生不等断裂情形，除玫瑰精油无影响，其余精油在UVA照光能量增加下皆有不等的DNA断裂情形发生，须再进一步试验。

Results Some spermatocytes all in model and clomiphere groups were denatured or death and spermatid did not develop.

结果：实验对照组的曲细精管上皮中多数精母细胞变性、死亡，精子细胞不发育，无精子形成，管腔中无精子，支持细胞有明显病变。

The spermatozoa are embeded in a extracellalar matrix composed of high dense fibrils matrix and flocculent and concentric vesicles similar to that in the primary spermatophore layer, but the later lack of spermotozoa. The flocculent and concentric vesicles are often seen to release their contents around the main body of spermatozoa. The spermatozoa may absorb the contents to form vesicular zone and membrane zone in sperm nucleu. The secondary spermatophore layer is distinctly subdivided into inner region and outer region. The loose outer region consists of canaliculated reticulum and large flocculent vesicles.

输精管内的精荚为管状，由精子群、精荚基质及初级精荚壁和次级精荚壁组成，初级精荚壁与精荚基质是相连续的，它们之间无明显界限，两者均由电子密度较高的纤丝状基质和絮状泡和同心圆泡组成，但后者不含精子，后者中的絮状泡和同心圆泡常在精子主体部周围释放内含物，可能与形成精子细胞核中的泡状带和膜状带有关，在交配后的雌虾，其头胸部腹面上粘着的精荚中，其基质内已不见絮状和同心圆泡，仅见稀疏的纤丝。

The ultrastructure characters of pollen grain in Brassica napus are:1There are vacuoles in cytoplasm but no starch grain in uniceelluar pollengrain.2The veget- ative nucleus of bicellular pollen grain is generally spheroidal;while the generative cell is spinde-shaped,with a big nucleus,thin layer of cytoplasmand few cell orgens,no cell wall.3In three-celled pollen grain the sperm cell is separated from the cytoplasm of vegetative cell by two layers of plasmic membrances.

Brassica napus的花粉粒，在不同发育时期超微结构的特征如下：1单胞花粉粒有一个球形的细胞核和明显的核仁，细胞质内出现液泡，缺少典型的淀粉粒。2双胞花粉粒的营养核多为球形。生殖细胞纺锤形，无细胞壁，细胞核比例大，细胞质呈薄层，细胞器少。3三胞花粉粒有一个裂片状的营养核和两个纺锤形的精细胞，精细胞无壁，以两层质膜与营养细胞的细胞质相隔。

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无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术，一种利用高压喷射技术，在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素，以雾态的形式定点、定量、定层、定位，以扇状的形直接、准确的到达皮肤中胎层，20分钟起效，2小时见效，48小时显效，无创无副作用，可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓，7天之内消除脂肪，重塑苗条身材，无恢复期，对棉布轮廓雕塑精准度可控制的毫米之间，不光是解决了衰老问题，可使原本漂亮的脸更加精致，维持之久。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

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Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

The results showed that (1) COX-2 mRNA and protein existed in the mature male rat testis. COX-2 was localized in spermatocytes by immunohistochemistry.(2) COX-2 also existed in adult man testis. COX-2 protein was positively stained in spermatocytes and Leydig cells.(3) 2 weeks after administration of rofecoxib, the level of testosterone in the whole testis reached its lower values, being only 50% of control values. However, testosterone level recovered during 4 weeks. After such treatment, histologic examination of these testes showed atrophy of the seminiferous tubules and maturation arrest of spermatogenesis.(4) 2 weeks post-EDS, expression of COX-2 decreased significantly (P.005), in comparison with vehicle-treatment control. 4 weeks after treatment, a new generation of fetal like Leydig cells repopulated in the testicular interstitium resulting in COX-2 expression partially recovered. Although the expression of COX-2 mRNA and protein are enhanced at 8th week after using external testosterone, it wasn't significant higher than control group.

实验研究证明：(1)正常成年雄性大鼠睾丸组织中COX-2在mRNA和蛋白质水平均存在表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞；正常成年男性睾丸组织中同样存在COX-2表达，免疫组织化学结果显示COX-2定位于曲细精管内的生精细胞和Leydig细胞；(2)服用特异性COX-2酶抑制剂rofecoxib 2周后，实验组大鼠睾丸组织内睾酮的含量减少，为正常对照组的50％；持续用药4周后睾丸组织内睾酮浓度逐渐恢复至正常水平；COX-2酶活性降低后病理组织切片显示睾丸内曲细精管萎缩，生精紊乱，持续用药4周时影响最明显；(3)注射特异性Leydig细胞杀灭剂EDS 2周后，实验组大鼠睾丸组织内睾酮浓度降至极低水平时，COX-2的蛋白和基因表达水平也显著低于正常空白对照组（P.005）；使用EDS后第4周，睾丸组织中的睾酮浓度逐渐回升，同样组织中COX-2蛋白表达和mRNA表达水平也相应提高接近正常水平；使用EDS后4周开始给予外源性睾酮，6周和8周时COX-2表达水平的绝对值虽有提高，但与正常大鼠空白对照组比较并无显著性差异，说明外源性雄激素刺激睾丸合成COX-2的作用并不明显。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。