无物

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

We look forward to the digital business card printing and membership card making the arrival of the new millennium, ink-jet technology because they will have no version, nonpressure, digital files, system stability, substrate features: wide, with its own technology to improve, in the digital environment, is showing very strong momentum of development.

我们等待着数字化制卡和会员卡制作新纪元的到来，喷不朱技艺由于其无版、无不抬、拔取数字文件、编制处事安定、承印物普及等特点，陪同其自身技艺的不停美满，在数字化不小境况下，侧表现不入极其强劲的成长势脚。

Results: 850 irrational prescriptions were identified (3.89%), mainly manifested in nonstandardized handwriting, discrepancies between pharmaceutical indication and diagnosis, repeated medication, no indication for the use of antimicrobial drugs, improper dosage, increased toxicity and pharmacological antagonism.

结果：不合理处方850张（占3.89%），主要表现在书写不规范、药品适应症与诊断不符、重复用药、无指征使用抗菌药物、用法用量不当、毒性增加、药理拮抗等方面。

A total of 304 patients were willing to choose Gongle IUD,of which 280 parous women and 24 nulliparous women were found no insertion contraindication following the gynaecological examination and the examination of vaginal discharge.

304例受术者均为自愿选择宫乐，其中经产妇280例，未孕妇24例，经妇检及阴道分泌物检查无放置禁忌证。放置后1、3、6、12

DSC measurements proved that the amorphous and crystalline prepolymerswere obtained catalyzed by stannous octoate and sulfric acid respectively.

DSC的分析表明以Sn2和浓硫酸为催化剂可以分别制备无定形的与结晶的聚乳酸/碳纳米管复合物。

It achieves a certain extent effect of waterproof and oilproof. 3. Test the change of surface ingredient before and after the fluoridized of the FRP usingthe reflecting method of infrared spectrum. Then dip the fluoridized FRP in the cold water, warm water and petroleum ether for about half an hour, and surface ingredient havent changed too much.

用红外光谱反射法测试了经含氟物处理前后的玻璃钢表面结构变化，并且分别测试了在冷水、温水、石油醚中浸泡洗涤半个小时后的表面含氟处理的玻璃钢的表面结构，发现其表面结构与浸泡前无大的变化。

Results All the children and their parents received interviews, otological examinations and clinical audiolugical teats. Their ages were between 4 and 15, with the average at 9.51±2.83.①The males were 341 (61.66%) and female 212 (38.34%), with the gender ratio of male to female as 1.61:1.②The peak of the age curves of treatment and the first onset of vertigo was 9.51 and 8.62-year-old, respectively.③20.98% of children with vertigo had normal results with peripheral vertigo more common in the rest.④ The semicircular canals were more likely to dysfunction if their relatives had vertigo or car sickness.

结果 ①553例儿童年龄4~15岁，平均9.51±2.83岁；男341例(61.66%)、女212例(38.34%)，男女之比为1.61：1；②就诊年龄和初次眩晕发病年龄的高峰期分别在9.51岁和8.62岁，发病无季节性，主要症状为自身或视物旋转，发作持续时间为数分钟至数小时；③约20.98%的儿童眩晕患者各项检查结果均正常，异常组中以外周性眩晕多见(78.04%， 341/437)，半规管功能异常者占63.83%(353/553)；④256例眩晕儿童直系家属中有眩晕史，其中171例(66.80%)出现水平半规管功能异常。

Overfamiliar yet always unknown. the enigma of Hamlet is emblematic of greater enigma of Shakespeare himself: a vision that everything and nothing , a person who was everyone and no one, an art so infinite that it contains us, and will go on enclosing those likely to come after us.

我们对他再熟悉不过，可又从不了解，哈姆雷特之谜象征着莎士比亚本身这个更大的谜；他是一个幻象，包罗所有，又空无一物；他是任何一个人，同时又谁都不是；他也是一门无穷无尽的艺术，&包容&了我们，并将继续容纳我们的后人。

Reaction of 9-bromofluorenewith diethyl malonate and sodium carbonate gives diethyl 2-(9-Fluorenyl)malonate.Aminolysis of diethyl 2-(9-Fluorenyl)malonate in ethylenediamine and propylene diamine give〓 and 〓, respectively.〓 and 〓 were synthesized by the reaction of 9-bromofluorene with oxalyl bisethylenediamine. Copper and nickel complexes of 〓 and 〓 were obtained by the reaction of 〓 and 〓 with copper and nickel salt, respectively, in aqueoussolution and extrusion of two hydrogen ions from the amide groups.

使9-溴芴与丙二酸二乙酯在无水碳酸钠存在下反应合成出2-(9-芴基)丙二酸二乙酯。2-(9-芴基)丙二酸二乙酯在乙二胺中胺解得到配体〓，2-(9-芴基)丙二酸二乙酯在1，2-丙二胺中胺解得到配体〓，使9-溴芴与草酰二乙二胺作用得到配体〓和〓与相应的金属盐作用分别得到它们的铜、镍配合物。

Methods Three kinds of special sequence primers for polymerase chain reaction with regard to the mutation styles (CGT、GTT、GAT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line Patu 8988.The amplification products were studied with 12% polyacrylamine gel eletrophoresis to detect the style of point mutation.

针对K-ras基因第12位密码子点突变方式(CGT、GTT、GAT)设计顺序特异性引物，对人胰腺癌细胞株Patu 8988进行聚合酶链反应，扩增产物经12%非变性聚丙烯酰胺凝胶电泳后判断该细胞株有无K-ras基因点突变及其突变方式。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。