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The results showed that Non-haemagglutinating activity was showed in the isolate. In agar diffusion test,there was a clear precipitin band formation to anti-IBDV specific serum. 28-day-old chicks inoculated with the isolate showed disease,and the isolate was recovered from the tissues of these chickens. The 1 041bp specific fragment were amplified by RT-PCR with sequence-specific primers based on IBDV VP3 gene. The VP3 gene shared 97.7%~98.2% nucleotide identities to very virulent IBDV,97.7%~95.2% nucleotide identities with classical strains from seqiemce analysis.

结果表明,该分离病毒无血凝性,在琼脂扩散试验中能与抗IBDV特异性血清出现1条清晰的白色沉淀线;人工感染28日龄雏鸡出现与临床一致的病变,并回收到病毒;应用针对IBDVVP3基因的特异性引物进行RT-PCR,能扩增到长度为1041bp的特异性目的片段;序列分析发现分离毒VP3基因与IBDV超强毒和经典毒株的核苷酸同源性分别为97.7%~98.2%和95.2%。

The CPP32 was activated and the 32-KDa proenzyme was cleaved. The active subunit p17 was detected at 3, 6, 9h of incubation and reached the peak at 6h.

用ICE的特异性荧光底物Ac-YVAD-AMC分析ICE活性,证明ICE在整个过程中均未见升高,与正常细胞组相比无显著性差异。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜,含类似上皮层和固有层,但细胞层次不清;胶原膜上可见口腔黏膜上皮细胞黏附生长,并形成复层上皮样组织,但无成熟的桥粒及基底膜样结构;以Alloderm作为支架材料的构建物,出现正常上皮形态,含半桥粒及细胞指状突起等超微结构。

Infection was identified by whether the surrounding skin color was red, swelling, heat or pain.

是否感染以针眼处分泌物的颜色及针眼周围皮肤有无红、肿、热、痛为标准,余护理同常规护理。

Glycine wasn't a repressible substrate for the reaction catalyzed by SHMT. The effect of concentration of glycine on reaction rate was consistent with M-M Equation. Under the conditions used in this research, V〓 was 0.212mmol/L/min and K〓 was 0.0198mol/L.

甘氨酸对丝氨酸羟甲基转移酶催化的合成反应无底物抑制作用,且甘氨酸浓度对酶反应速度的影响服从米氏方程的形式,实验条件下V〓为0.212mmol/L/min,K〓为0.0198mol/L。

The protein had the specificity on substrate recognition, which could decompose 3,4-dihydroxybenzoic acid to catechol but not 2,4-dihydroxy- benzoic acid to resorcin. The ligand at C-3 site of benzene ring had no effect on enzyme activity.

蛋白质还具有底物识别的特异性,能够催化3,4-二羟基苯甲酸转化为邻苯二酚,而不能催化2,4-二羟基苯甲酸转化为间苯二酚,这表明苯环上3'位的配基对酶活无影响。

Main business: Sales Main Products: Temperature instrumentation, pressure instrumentation, flow meters, material / liquid level instruments, metering equipment, control systems thermocouples, thermal resistors, wire compensation, pressure gauge, transmitter, pressure transmitter, Smart Transmitter, Level Transmitter, Flow Meter, Orifice flowmeters, electromagnetic flowmeter, vortex flowmeter, Verabar, metal tube rotameter, gas flowmeter, no NG Paperless Recorder, indicating regulator, regulator, hand fuck, and Flow Totalizer, level transmitter, pressure calibrator, process calibrator, digital pressure gauge, check simulation device, auto-calibration system.

主要业务∶销售主营产品:温度仪表,压力仪表,流量仪表,物/液位仪表,计量仪器,控制系统热电偶,热电阻,补偿导线,压力表,变送器,压力变送器,智能变送器,液位变送器,流量计,孔板流量计,电磁流量计,涡街流量计,威力巴流量计,金属管浮子流量计,气体流量计,无仪纸记录仪,显示调节仪,调节器,手操器,流量积算仪,液位变送器,压力校验仪,过程校验仪,数字压力计,校验仿真仪,自动校验系统。

The lesion area in the vulva was washed with warm water to remove secretion on the ruga. If there was ulcer or erosion in the lesion area, the wound was washed with 3% hydrogen peroxide and normal saline. MEBO was applied locally to a thickness of 2 ~ 3 mm and bandaged in sterilized way. If there was no ulcer, MEBO gauze was applied to the vulva.

用温水清洁外阴皮肤及皱襞上的分泌物,如有破溃,糜烂面,再以3%过氧化氢清创,生理盐水清洗创面及周围皮肤,将MEBO涂于创面上,厚约2~3㎜,无菌包扎;非破溃创面,以MEBO制成药纱敷外阴,每次排尿、便后换药。

Magician shows pan empty, he breaks an egg into the pan, adds salt, pepper and "vinegar"(it's really lighter fluid).

魔术师展出一只空无一物的镬子,他把鸡蛋打进去,并加了一些盐、胡椒粉及「醋」。

Is what is savorless eaten without salt?

6:6 物淡而无盐,岂可吃么?

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。