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And the Content of chlorophyl, soluable sugar, root system vigor of T3,T4 treatment is the relative fine in all treatments, it has no distinct difference with CK.

而且T4,T3处理番茄幼苗的叶绿素含量、可溶性糖、根系活力在所有处理中表现较好,和CK无显著差异。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

We see round in a new globle, full of life, and motion, and ceaseless progress; and feel in ourselves all the vigor and spirit to hold pace with it, and do not foresee from any present symptoms how we shall be left behind in the natural course of stuff, decline into old age, and drop into the grave.

咱们看到四周一派新天地——生机盎然,变动不居,日新月异;咱们觉得本人活力充盈,精神饱满,可与宇宙并驾齐驱。而且,眼前也无任何迹象没出国疑问证明,在大自然的进展过程中,咱们本人也会落伍,衰老,进入坟墓。

We see round in a new globle,full of life,and motion,and ceaseless progress;and feel in ourselves all the vigour and spirit to hold pace with it,and do not foresee from any present symptoms how we shall be left behind in the natural course of stuff,decline into old age,and drop into the grave.

咱们看到四周一派新天地——生机盎然,变动不居,日新月异;咱们觉得本人活力充盈,精神饱满,可与宇宙并驾齐驱。而且,眼前也无任何迹象没出国疑问证明,在大自然的进展过程中,咱们本人也会落伍,衰老,进入坟墓。

The incidence of anxiety and depression in patients with FD was higher than that in healthy volunteers; The average extent of anxiety and depression of FD patients was significantly higher than that of healthy people (p 0.001). 2.The total scores of life events and the scores of negative life events in patients with FD was significantly higher than that of healthy group (p=0.014 and p 0.001), but the favorable life events scores was lower than healthy group's(p=0.001). 3. Eight healthy conceptions in the health-related quality of life of FD patients was significantly lower than that of healthy volunteers (p .001). 4. There was positive correlation among the symptom iterated integral, anxiety evaluation and depression evaluation of the FD patients (p0.001). 5. In the FD patients, there was no significant correlation between the evaluation of symptoms and life events. 6. In the FD patients, there was no significant correlation among symptoms evaluation, energy and body ache, and negative correlation among the other 6 health conceptions (p 0.05). 7. There was significant positive correlation among the total scores of life events、negative life events scores and anxiety evaluation of the FD patients(p.01),and also with depression evaluation(p.05),there was no significant correlation among the favorable life events scores with anxiety and depression evaluation both. 8. There was negative correlation among anxiety evaluation, depression evaluation and 8 health conceptions of the FD patients (p 0.001). 9. There existed negative correlation respectively among the total scores of life events and negative life events scores with 6 health conceptions of the FD patients (p 0.05) and 7 health conceptions of the FD patients (p 0.05), there was no significant correlation among the favorable life events scores and the health-related quality of life of FD patients.

结果:1、FD患者焦虑、抑郁的发生率明显高于健康对照组(p.005),平均焦虑、抑郁程度高于对照组(p.001)。2、FD患者经历的生活事件总值(p=0.014)和负性生活事件值(p.001)明显高于健康对照组,正性生活事件值低于对照组(p=0.001)。3、FD患者的生活质量在8个健康概念上均明显比健康人差(p.001)。4、FD患者的症状积分与焦虑评分、抑郁评分呈正相关(p.001)。5、FD患者的症状积分与生活事件值均无相关性。6、FD患者症状积分与活力、躯体疼痛之间无明显相关性,与其余6项健康概念均呈负相关(p.05)。7、FD患者的生活事件总值、负性生活事件值与焦虑评分均显著正相关(p.01),与抑郁评分也均呈正相关(p.05),正性生活事件值与焦虑和抑郁水平均无相关性。8、FD患者焦虑评分、抑郁评分与8项健康概念均呈负相关(p.001)。9、FD患者生活事件总值和负性生活事件值分别与生活质量8项健康概念中的6项和7项呈负相关(p.05),正性生活事件值与生活质量无关。

Your country baffles me: a luxurious unharmed lotus land in which great hordes of handsome dynamic people either wallow in deep gloom , or play like overexcited children, or fall to work like all the devils in hell, while the press steadily drones detestation of the government and despair of the system.

你们的国家真叫我觉得不可思议:仙境般无忧无虑的国度,容光焕发、活力充沛、熙熙攘攘的人群,他们不是沉溺在忧郁悲观的深渊中,就是像欣喜雀跃的儿童一般游戏作乐,要不然就像入地狱的鬼魂一般辛苦工作,而你们的报纸则是无休无止地指责政府,宣称你们的制度无可救药。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P<0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P>0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6%final gel concentration and there were 15~19 bands identified on this condition.

同功酶电泳图谱显示,1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著,均以LDH-5为主,但1℃组LDH-1显著高于室温组(P<0.05),推测黑龙江林蛙为适应低温低氧环境,增强LDH-1酶活性以较好的利用乳酸,避免因大量乳酸形成造成肝脏疲劳;而1℃组肝脏G6PDH酶活性无显著变化,进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调,维持机体代谢的稳定;1℃组和室温组EST总酶比活力无显著变化,但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P<0.05),推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性,增加肝脏酯类物质代谢,为机体提供维持生命必需的最低能量。

Objective: To investigate acusing zu san li's influence on the content of hydroxyproline and Malondialdehyde in the skin of hireless photoageing mice as well as the activity of Superoxide Dismutase in their skin,and to investigate milkvetch's effect on the structure of the hireless photoageing mice.

目的:本项实验旨在研究针刺足三里对紫外线照射引起的无毛小鼠皮肤光老化模型皮肤组织羟脯氨酸含量、丙二醛含量、超氧化物歧化酶活力,以及无毛小鼠真皮结构变化的影响。

The molecular weight of dextransucrase measured by SDS-PAGE was 170 kDa ,which was similar to the enzyme from Leuconostoc mesenteroides. The enzyme had an optimum temperature between 25and 30℃ and an optimum pH of 5.4. It was relatively stable in the range of pH 5.0 to 7.0, but the stability declined rapidly as soon as the temperature rose over 35℃.

以蔗糖为底物,酶促反应的最适温度为25~30℃,最适pH值为5.4,动力学常数Km值为10.43 mmol/L;酶活在pH 5.0~8.0较为稳定,在室温(25℃)保藏4 天仍有59%的酶活力,4℃保存7周酶活力仅下降一半,但在35℃以上失活很快;Ca2+对催化作用有较大的促进,Mg2+有微弱的促进作用,K+对催化反应无影响,Cu2+的抑制作用最强。

67 of old person latent to split tooth carry on clinical diagnosis, adopt the complex treatment method according to different situations to 63. 4 shallow form latent to split tooth, dental pulp normal having subjective symptoms to be only competent to transfer tooth treat jointly; Have deeper crackle as to 5, the vigor of dental pulp is normal, but there is trouble tooth of dentine irritated symptom, prepares against the hole, is filled with on the crack while adjusting the tooth contract; Pulpitis, 22 58 drops of trouble tooth with the sharp disease, symptom of the root already appear to 36, go ahead of the rest steel wire ring pitch or make, bring ring bonding in order to protect corona, rub except that high sharp abrupt slope, after dispelling the tooth and shutting the wound, the root canal with complete regeneration, observe for 2 weeks, after there is no clinical symptom, preceded and repair completely in time.

对63例老年人67颗隐裂牙进行临床诊断,并根据不同情况采用了综合治疗的方法。4颗浅表的隐裂牙,牙髓正常且无自觉症状仅行调牙合治疗;对于5颗有较深裂纹,牙髓活力正常,但有牙本质过敏症状的患牙,调牙合同时在裂隙处备洞、充填;对36颗已出现牙髓炎、22颗伴有根尖病症状的58颗患牙,先行钢丝环扎或做带环粘结以保护牙冠,磨除高尖陡坡,消除牙合创伤后,再行完善的根管治疗,观察2周,无临床症状后,及时做全冠修复。

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