无活力

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs by MTT detecting method.

MTT法检测发现GFP和SPIO双标记神经干细胞的增殖活力与未标记神经干细胞相比无改变。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

More importantly, street dances are characterized by emotions simply not found in the ballroom dance: passion, sex, exuberance, and unrestraint.

更重要的是，街舞有着与标准舞不一样的特点：激情、性感、活力四射和自由无拘束。

Different responses of seed germination physiology in mung bean and common vetch towards Cd2+ treatment were conducted with an experiment.

采用室内试验方法，研究了绿豆和箭舌豌豆的种子萌发对不同浓度Cd2+处理的响应。结果表明，较低浓度的Cd2+(低于50或100μmol·L-1)处理对这两种作物的种子发芽率和发芽系数无明显的影响，但影响其活力指数。

Nature sent him into the world strong and lusty, in a thriving condition, wearing his own hair on his head, the proper branches of this reasoning vegetable, till the axe of intemperance has lopped off his green boughs, and left him a withered trunk, he then flies to art, and puts on a periwig, valuing himself upon an unnatural bundle of hairs, all covered with powder, that never grew on his head; but now should this our broomstick pretend to enter the scene, proud of those birchen spoils it never bore, and all covered with dust, through the sweeping of the finest lady's chamber, we should be apt to ridicule and despise its vanity.

刚来到这个世界上时，它是多么的遒劲有力，多么的活力十足，欣欣向荣。头上长着与生俱来的嫩枝，枝叶繁茂，然而，人类的斧头无节制地将它的枝干砍掉，直到留给它一个枯萎的树干。然后，它只得求助艺术，戴上一丛自己看来并不自然的&假发&，而且那假发永远也不会生长。自己则完全掩埋在灰尘中。但是，我们的扫柄也应这样登场——把桦木的枝条安在自己身上，并且为之洋洋自得吗？它们身上覆盖了尘土，尽管还是最尊贵的夫人房里的尘土。即便这样，它的虚荣心，也应该受到我们的嘲笑与鄙视。

Seed vigor and drought resistance of three perennial forages belong to Bromus (Bromus ciliatus, Bromus inermis, Bromus ircutensis) in the period of seed germination were compared under PEG-6000 of different levels drought stress.

用不同浓度的PEG-6000溶液模拟干旱胁迫，比较雀麦属3种多年生牧草（缘毛雀麦、无芒雀麦、沙地雀麦）种子萌发期的种子活力与抗旱性。

The different density gibberelin GA3 and the polyethyleneglycol PEG can increase the reduced vigor index of the seed Celosia Cristata L., but it is not remarkable compared with ck, the treatment with 50mg/L PEG is favorable.

不同浓度的GA3和PEG处理对鸡冠花种子的简化活力指数都略有提高，但与对照相比均无显著差异，效果最佳的是50mg/L PEG处理。

Coracoclavicular ligament injury repaired by tissue engineered tendon is a feasible method.

结论用组织工程学原理构建的人工肌腱修复喙锁韧带损伤是可行的手术方法，植入的同种异体细胞至少在6个月内保存了活力，无明显排斥反应。

The arrangement of odontoblasts was found in line and there was no inflammation. The root pulp and periodontal tissue were normal. Finally the opened area was closed by dentinal bridge.

周边造牙本质细胞排列整齐，牙髓无炎症及变性，根髓及牙周组织正常，最终形成牙本质桥将穿髓孔封闭使牙髓保存了活力。

Activities of phenylalanine ammonia - lyase, cinnamic acid 4 - hydroxylase and diterpene cyclase in rice plants are induced when their leaves are irradiated with UV light for 12 hours and 24 hours.

紫外光处理水稻化感材料后12 h和24 h，被诱导的苯丙氨酸氨基裂合酶、肉桂酸-4-羟化酶(CA4H)和二萜环化酶的酶活力比紫外光处理的无化感作用水稻中的要高2-4倍。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了