无活力

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

The incidence of anxiety and depression in patients with FD was higher than that in healthy volunteers; The average extent of anxiety and depression of FD patients was significantly higher than that of healthy people (p 0.001). 2.The total scores of life events and the scores of negative life events in patients with FD was significantly higher than that of healthy group (p=0.014 and p 0.001), but the favorable life events scores was lower than healthy group's(p=0.001). 3. Eight healthy conceptions in the health-related quality of life of FD patients was significantly lower than that of healthy volunteers (p .001). 4. There was positive correlation among the symptom iterated integral, anxiety evaluation and depression evaluation of the FD patients (p0.001). 5. In the FD patients, there was no significant correlation between the evaluation of symptoms and life events. 6. In the FD patients, there was no significant correlation among symptoms evaluation, energy and body ache, and negative correlation among the other 6 health conceptions (p 0.05). 7. There was significant positive correlation among the total scores of life events、negative life events scores and anxiety evaluation of the FD patients(p.01),and also with depression evaluation(p.05),there was no significant correlation among the favorable life events scores with anxiety and depression evaluation both. 8. There was negative correlation among anxiety evaluation, depression evaluation and 8 health conceptions of the FD patients (p 0.001). 9. There existed negative correlation respectively among the total scores of life events and negative life events scores with 6 health conceptions of the FD patients (p 0.05) and 7 health conceptions of the FD patients (p 0.05), there was no significant correlation among the favorable life events scores and the health-related quality of life of FD patients.

结果：1、FD患者焦虑、抑郁的发生率明显高于健康对照组(p.005)，平均焦虑、抑郁程度高于对照组(p.001)。2、FD患者经历的生活事件总值(p=0.014)和负性生活事件值(p.001)明显高于健康对照组，正性生活事件值低于对照组(p=0.001)。3、FD患者的生活质量在8个健康概念上均明显比健康人差(p.001)。4、FD患者的症状积分与焦虑评分、抑郁评分呈正相关(p.001)。5、FD患者的症状积分与生活事件值均无相关性。6、FD患者症状积分与活力、躯体疼痛之间无明显相关性，与其余6项健康概念均呈负相关(p.05)。7、FD患者的生活事件总值、负性生活事件值与焦虑评分均显著正相关(p.01)，与抑郁评分也均呈正相关(p.05)，正性生活事件值与焦虑和抑郁水平均无相关性。8、FD患者焦虑评分、抑郁评分与8项健康概念均呈负相关(p.001)。9、FD患者生活事件总值和负性生活事件值分别与生活质量8项健康概念中的6项和7项呈负相关(p.05)，正性生活事件值与生活质量无关。

Your country baffles me: a luxurious unharmed lotus land in which great hordes of handsome dynamic people either wallow in deep gloom , or play like overexcited children, or fall to work like all the devils in hell, while the press steadily drones detestation of the government and despair of the system.

你们的国家真叫我觉得不可思议：仙境般无忧无虑的国度，容光焕发、活力充沛、熙熙攘攘的人群，他们不是沉溺在忧郁悲观的深渊中，就是像欣喜雀跃的儿童一般游戏作乐，要不然就像入地狱的鬼魂一般辛苦工作，而你们的报纸则是无休无止地指责政府，宣称你们的制度无可救药。

The molecular weight of dextransucrase measured by SDS-PAGE was 170 kDa ,which was similar to the enzyme from Leuconostoc mesenteroides. The enzyme had an optimum temperature between 25and 30℃ and an optimum pH of 5.4. It was relatively stable in the range of pH 5.0 to 7.0, but the stability declined rapidly as soon as the temperature rose over 35℃.

以蔗糖为底物，酶促反应的最适温度为25～30℃，最适pH值为5.4，动力学常数Km值为10.43 mmol/L；酶活在pH 5.0～8.0较为稳定，在室温（25℃）保藏4 天仍有59%的酶活力，4℃保存7周酶活力仅下降一半，但在35℃以上失活很快；Ca2+对催化作用有较大的促进，Mg2+有微弱的促进作用，K+对催化反应无影响，Cu2+的抑制作用最强。

Malondialdehyde production and total superoxide dismutase activity in samples of nondependent lung and dependent lung were detected and analysed in both groups on time of OLV 3hr and OLV 6hr to determine whether long-term OLV led to injury of the nondependent lung and whether the injury would be deteriorated by low-dose ALM.

通过对OLV 3hr及OLV 6hr时两组实验犬无通气肺与通气肺组织标本中丙二醛含量及总超氧化物歧化酶活力的测定，观察长时间OLV是否引起无通气肺组织损伤，并同时探讨小剂量ALM对无通气肺组织损伤的影响。

Methods Spectrophotometry was used to study the activity of PLAP in the presence and absence of L Leu. PLAP relative activity was calculated according to the absorbance variation.

方法应用分光光度法分别测定有L－Leu和无L－Leu存在下的酶活力，根据其活力变化求出酶的相对活力；用双倒数作图法，确定L－Leu对PLAP的抑制类型并求出其抑制常数；同时也观察了pH对抑制作用的影响。

In Chapter Two, some methods such as electron microscope, DNA ending label and MTT measurement were used to studied the changes of apoptosis, necroses and cell vigour of leukaemia K562 cell trunk and HL-60 cell trunk irradiated with millimeter wave. Different effects of millimeter wave to leukaemia cell trunks and human marrow cells were observed clearly. Human marrow cells were not so sensitive to millimeter wave radiation as leukaemia cells.

第二章，利用电镜技术、DNA末端标记、MTT活力测定等方法，研究了毫米波对红白血病K562细胞株、人类急性早幼粒白血病细胞株HL-60及正常人骨髓单核细胞的细胞超微结构、细胞凋亡、细胞活力的影响，结果发现毫米波辐射对正常人骨髓细胞及白血病细胞的影响明显不同，正常人骨髓细胞所受影响较小，特别是功率密度小的毫米波对其无影响；而白血病细胞受辐射后发生显著的凋亡或坏死，细胞活力显著下降，该效应具有明显的功率窗和时间窗。

The result shows: in 0~360 kg/hm^2 fertilizer can improve the plant height, dry matter accumulation of maize, nitrogen accumulation, root vigor, leaf nitrate reeducates activity, chlorophyll content and yield, meanwhile the leaves' nitrate reeducates activity, dry matter accumulation and the final output of wheat, root activity and other indicators were significantly correlated; Cultivating under the same conditions and dropping the fertilizer amount from 360 kg/hm^2 to 315 kg/hm^2, the dry matter accumulation will be increased, the roots vigor and leaf nitrate reeducates activity will be strengthened, but the yield are not obviously different in the same treatment.

结果表明：在0~360kg/hm^2的施肥范围内，肥料能显著增加玉米植株的株高、干物质积累盆、叶绿素含量和籽粒产量，提高根系活力、叶片硝酸还原酶活性；同时硝酸还原酶活性、干物质积累量、根系活力和叶绿素含量等指标与玉米最终产量呈显著正相关；在相同的栽培条件下，将施肥量从360kg/hm^2降至315kg/hm^2，植株的于物质积累量增加、根系活力、叶片硝酸还原酶活性增强，但处理间籽粒产量无显著差异。

6 Days after plate culture, the cell number and cell viability of PLGA microspheres was much better than that of PELA microspheres and free bFGF.

4培养1天后，四组细胞计数、活力均无明显差异，4、6天PLGA组细胞计数和活力优于PELA组和游离bFGF组。

Function description: The new skin energy-refreshing lotion, which contains multi-peptide essence to supply nutrition required by skin, motivates energy of cells, and even could pertinently generate oxygen for long, obviously increase oxygen level of cells, reduce wrinkles, give you firm skin without any aging mark.

功能说明：质感柔和且效果卓越，让肌肤活力再生的精华露，蕴含多肽精华，能够补充肌肤所需营养，激发细胞活力，更有针对性长效释放氧，明显提高细胞含氧量，减少皱纹，令肌肤紧致无痕。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。