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We examined the metabolic change in responses to anoxia in American cockroach, Periplaneta Americana by measuring the O2 consumption, CO2 production and respiratory quotient.

本研究测量美洲蟑螂的氧气消耗量、二氧化碳排出量与呼吸商,以探讨缺氧对其代谢的影响,连续在氮中缺氧超过38小时的蟑螂,除活动力下降外,行为上无明显的变化。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

Results of edaphon diversity by DGGE analysis of soil DNA showed that both bacteria and fungi Shannon index of treatment N_U were lower than those of treatment No, which suggested that planting crop decreased the soil microbial Shannon index.

油菜地各处理的土壤N_2O、CO_2排放通量高于小麦地相应处理,常规处理和无氮处理的土壤N_2O、CO_2排放通量高于空闲地处理,说明作物的生长促进了土壤温室气体的排放。

The reason for the fail of carbon nitride synthesis was analysed and relevant graphitoid carbon nitride and unformed carbon nitride s were introduced in the article.

分析了妨碍碳氮化物合成成功的原因,对相关的类石墨的碳氮化合物和无定型的碳氮化合物作了介绍。

Mainly engaged in sodium azide, barium azide, three phosphorus nitride Friday, phenyl isothiocyanate, 1 - phenyl -5 - mercapto-tetrazole, 4 - dimethylamino-pyridine, 2 - mercapto--5 -- methyl-1,3,4 thiadiazole, N-hydroxy phthalimide, anhydrous betaine and betaine hydrochloride, such as fine chemical raw materials and medicine, pesticide intermediates of the development, production and sales.

主要从事叠氮化钠,叠氮化钡,五氮化三磷,异硫氰酸苯酯,1-苯基-5-巯基四氮唑,4-二甲氨基吡啶,2-巯基-5-甲基-1,3,4噻二唑,N-羟基邻苯二甲酰亚胺,无水甜菜碱及甜菜碱盐酸盐等精细化工原料及医药、农药中间体的开发、生产和销售。

The RE of TP, COD and turbidity except NH4+-N in the aeration process by adding AS and PAM for enhanced chemical phosphorus removal increased by 7.3~59.2%, 5.0~20.3% and 10.9~34.7%, respectively. Given enough dissolved oxygen, the nitrification was not affected by adding AS and PAM in the dosage range of the study. The RE of TP, COD and turbidity by postpose coagulation were better than by simultaneous coagulation, however, coagulation and sedimentation equipments were needed in postpose coagulation process. Therefore simultaneous coagulation was more suitable for transformation of AB process for chemically enhanced phosphorus removal.

曝气过程中投加AS和PAM复配化学强化除磷,总磷、COD、浊度去除率分别提高了7.3~59.2%、5.0~20.3%、10.9~34.7%,但不能提高氨氮的去除率;在溶解氧足够时,本研究投加量范围的AS和PAM的加入对硝化作用无影响;后置混凝对TP、COD、浊度的去除效果优于同步混凝,但需增加混凝沉淀设备,因此同步混凝更适合于于AB工艺的化学强化除磷改造。

The result indicated that crude protein content was controlled by additive and no-additive effects, and crude fat, crude fiber and nitrogen-free extract were controlled by dominant effects, whereas crude ash was controlled additive and dominant effects.

结果表明,粗蛋白的遗传受加性、显性和上位性及环境效应控制,粗纤维、粗脂肪和无氮浸出物的遗传主要受显性效应控制,粗灰分的遗传受加性和显性效应共同控制。

The results showed that there were significant differences between two different periods of pigs (P.05) in apparent digestibility value of crude protein, crude fiber, nitrogen-free extract, ether extract.

结果表明,在20~50㎏阶段,除虫菊花渣的粗蛋白、粗纤维、无氮浸出物和粗脂肪的表观消化率分别为45.78%、24.27%、79.49%和53.55%,表观消化能为8.53MJ/㎏;在60~90㎏阶段,其CP、CF、NFE和EE的表观消化率分别为56.29%、40.52%、79.66%和64.62%,DE为9.16MJ/㎏。

Method] Solid and liquid medium of the nitrogen-free BAP, S, JA, ISP4, CB-CcI3, DPM, FTW were used to isolate and purify root nodules of Elaeagnus and Hippophae collected from Tongliao and Chifeng in Inner Mongolia. According to determination results of 16S rDNA sequence, a phylogenetic analysis was carried out.

方法]以通常用于分离弗兰克氏菌属固氮放线菌的无氮BAP、S、JA、ISP4、CB-CcI3、DPM和FTW等为固体和液体培养基,对采集自内蒙古通辽和赤峰的沙棘和沙枣植物根瘤进行放线菌的分离纯化,并依据16S rDNA序列测定结果对分离物进行系统发育分析。

The observation and monitoring were carried out for one year. Improvements resulted from the project include suspended particles, transparency, concentration of chlorophyll A, total organic carbon, total nitrogen. Factors that did not show obvious improvement include electrical conduction, concentration of nitrate nitrogen, ammonia nitrogen, nitrous nitrogen, phosphate salts. Finally, factors that were influenced by environment include water level, temperature, pH, total phosphorus, chloride salts.

历经一年的试验,因设备投入而改善之因子为溶氧、浊度、悬浮固体、透明度、叶绿素a、总有机碳、总氮等7项,无明显变化之项目为导电度、硝酸盐氮、氨氮、亚硝酸盐氮、磷酸盐等5项,受环境影响因子为水位、水温、pH、总磷、氯盐等5项。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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