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We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

When countrybound velocipedes, a chainless freewheel roadster cycle with side basketcar attached, or draught conveyance, a donkey with wicker trap or smart phaeton with good working solidungular cob (roan gelding, 14 h).

下乡的时候,就骑老式脚踏车,挂有柳条编的车斗的无链飞轮跑车,要么就是牲口拉的车,柳条车身的二轮轻便驴车或是脚步矫健飞快的短腿壮马(骗过的灰斑栗毛马,身高十四掌尺)所拉的时髦的四轮轻便马车。

Culm sheaths yellow-green or pale red-brown, becoming straw-colored, with variably sized brown spots, base edged with white pubescence; auricles and oral setae absent; ligule yellow-green, truncate or weakly convex at apex, very short, 1–2 mm, margin longer pale green ciliate; blade reflexed, green, with yellow margins, linear, flat or crinkled in upper sheaths.

竿箨黄绿色或者苍白的红棕色,变得稻草色,带有易变依大小排列的棕色的点,基础随着白色的青春期推进;叶耳和口头的刚毛无;叶舌黄绿色,削去或者身体虚弱凸在先端,非常短,1-2毫米,边更长的苍绿色纤毛虫;反折,绿色的叶片,具黄边缘,在上面鞘内皱曲的线形,平或。

Butterfly, a symbol of what a wonderful dream, cat, dog, red, ice, snow, gentle, love is perhaps the subconscious of the young painter Perishable's Lament, the longing for love, for self-dual or cold lonely heart ridicule, Kogusu, daffodils Years Old Little Love, a white flower buttercup, blue water rafting around the situation so much a painter's friend, rather, is with the self-life aesthetic of beauty, purity, free abstract ideal reflection and youth Imagine interpretation, these works reveal the artist in the virtually real and realistic three-dimensional puzzle, showing the deep spirituality of individual aesthetic white, innocence, kindness, fantasy, sincere, innocent and beautiful.

蝴蝶,多么美好的梦的象征,猫咪、小狗、红线、冰、小雪、温柔、爱或许正是画家潜意识对青春易逝的感喟、对爱的渴望、对自我内心孤寂的偶或冷嘲,小楠、水仙、小娜、小爱、白色花毛茛、碧波泛舟与其说是画家周遭情境中的朋友,毋宁说是对自我生活审美与对美好、纯洁、自由的理想化抽象观照与青春畅想演绎,这些作品于无形中透漏着画家的立体真实和现实困惑,透出了审美个体性灵深处的洁白、无邪、善良、梦幻、真诚、天真和美丽。

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