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The Ca〓 oscillations occured before GVBD were associated with the resumption of GVBD, because when the oocyte was cultured in medium with hypoxanthine, no Ca〓 oscillation occurred, without hypoxanthine the oscillations occured again.

这些Ca〓波动与卵母细胞的GVBD有关,被次黄嘌呤抑制于GV阶段的卵母细胞不出现这种形式的波动,当再次培养于无次黄嘌呤的培养液中则又出现Ca〓的波动。

Results Some spermatocytes all in model and clomiphere groups were denatured or death and spermatid did not develop.

结果:实验对照组的曲细精管上皮中多数精母细胞变性、死亡,精子细胞不发育,无精子形成,管腔中无精子,支持细胞有明显病变。

Results The diagnostic correspondent rate of obstructive and nonobstructive azoospermia by seminal spermatogenic cells examination was 85%, 95.1% respectively. The spermatogenic arrest at spermatid level, spermatocyte level and spermatogonium level or sertoli cells only were 14, 21 and 6 cases respectively.

结果 精液生精细胞学检查对梗阻性和非梗阻性无精症分型诊断的符合率分别为85%、95.1%。41例非梗阻性无精症中,生精阻滞阶段在精子细胞、精母细胞、精原细胞分别为14例2、1例和6例。

24H treatment group compared with 24h model group: the level of MCP-1 of the Gardenoside group decreased, but the level of MIP-1 of it is no different with the model group; the level of the MCP-1 of the Baicalin group increase and the level of MIP-1 of it all decreased; the level of MCP-1 and MIP-1 of the Concha Margatitifera Usta all decreased; the level of MCP-1 of the Cholic Acid group decreased and the level of MIP-1 is no different with the model group; the level of MCP-1 and MIP-lof the Hefang Group decreased than the model group.

在本实验中,脑缺血能够诱导P53蛋白表达,P53蛋白可能参与调控缺血后神经细胞延迟性死亡的病理生理过程,药物通过抑制细胞凋亡,从而发挥治疗作用。五、大鼠局灶性脑缺血12小时模型组缺血脑组织HSP70光密度值含量较正常组明显增高(p<0.05)。珍珠母治疗脑缺血12小时组缺血脑组织HSP70光密度值较缺血12小时模型组无显著性差异(p>0.05)。胆酸治疗脑缺血12小时组缺血脑组织HSP70光密度值较缺血12小时模型组无显著性差异(p>0.05)。

However, zona hardening could occur during in vitro maturation of oocytes in a simply defined medium which reduced the fertilization rate.

采用无蛋白培养基对卵母细胞进行体外成熟培养,并防止透明带硬化,是研究影响卵母细胞成熟过程因素的基础。

Results There was not specificity of clinical symptoms in liposarcoma of the orbit, the major clinical aspects were intraorbital occupied lesion and local hyperaemia,swell and pain which was similar to an acute inflammation. Four different subtypes of liposarcomas could be observed in orbit.In past literature,myxoid was the most common subtype(14 cases,70%),secondly subtype was well differentiated(3 case),round cell and pleomorphic subtype were rare(each 1 case).This case was well differentiated mixed component of round and pleomorphic lipoblasts.

结果:脂肪肉瘤临床表现为眶内占位和貌似急性炎症的局部红、肿、痛,无特征性的症状;病理分型4型,文献中报道以粘液样型最多见(14例,占70%);次为分化良好型,3例;圆形细胞为主型及多型性各1例;分型不详2例;本例为分化良好型为主伴有圆形脂母细胞及多型性脂母细胞成分。

Results Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP. It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts.

结果 肌样/肌纤维母细胞性分化多出现在纤维肉瘤型DFSP中,表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短条束,由梭形细胞组成,细胞多无异型性,核分裂象也罕见,形态上似平滑肌细胞或肌纤维母细胞。

In the PbⅠ stage oocyte, perivitelline enlarged further, the cumulus cells expanded and the cell junctions disappeared. On the membrane of oocyte near the first polar body, a no microvillus area was observed, where probobly was the position for the second polar body extruding.

在PbⅠ阶段的卵母细胞内,卵周隙进一步扩大,卵丘细胞扩散,无细胞间连接存在,卵母细胞表面靠近第一极体的部位存在无微绒毛的区域,可能是第二极体释放的部位。

The results showed that the expression of calm3 and trm112l genes had no remarkable differences in oocytes and 8-cells stage embryos, but were significantly different in blastocysts. It was supposed that these two genes were possibly maternal genes, and they might be involved in oocyte maturation and zygotic genome activation.

结果显示,在卵母细胞和8细胞期胚胎,基因calm3和trm112l的表达量无显著性差异,而在囊胚期的表达量与前两个时期的表达量存在显著性差异,推测这两个基因在牛胚胎发育过程中的表达可能属于母型调控,提示该基因在卵母细胞成熟和合子基因激活中起一定作用。

The feasibility of maturing denuded buffalo oocytes in vitro was explored in this study, so as to provide a model to investigate the mechanisms of oocytes maturation.

主要探讨无卵丘水牛卵母细胞体外成熟的可行性,以便为研究卵母细胞成熟机理提供模型。

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