无极

It was found that only few log-phase trophozoites were labeled by means of C6-NBD-ceramide, however, most of the encysting trophozoites were labeled in perinuclear region.

结果 极少数蓝氏贾第鞭毛虫滋养体被C6-NBD神经酰胺标记；多数处于成囊6、12、18h时段的虫体均可见被特异标记成亮绿色的核周围区域。golgin245和Rab1在滋养体及成囊诱导6、12、24h的虫体中的表达水平无显著变化。

Results When CT imaging showed pancreatic density uniformity and no peripancreatic fluid collection,the pancreatitis recurred rarely following DAR therapy for 3 days,and when CT imaging showed pancreatic density asymmetry and peripancreatic fluid collection,it needed DAR therapy for 14 days.

结果如果CT表现为胰腺质地均匀、胰周无渗出，DAR疗法连用3 d，停药后复发的概率很小。如果CT表现为胰腺质地不均、胰周有渗出，DAR疗法连用14 d，停药后复发的概率极小。

METHODS: Questionnaire: SCL-90 was used to evaluate 9 main outcome measures, named psychological health, somatization, obsessive compulsive symptoms, personal relations, depression, anxiety, hostility, phobism,paranoid ideation and metal disorder; in which, 5 grades were adopted,named 1, 2, 3, 4 and 5 grades, to represent no, mild, moderate, severe and extremely severe impacts successively.

问卷调查：采用症状自评量表评估学生的心理健康状况，以躯体化、强迫症状、人际关系、抑郁、焦虑、敌对、恐怖、偏执和精神病性等9项因子为主要观察指标，采用5级评分，1，2，3，4，5分别表示无、轻、中、重、极重。

Result: The yield of S4 was 18.63 % and 29.99 % higher than that of CK1 and CK2, respectively. The contents of volatile oil and pulegone were also higher than those of CK and other strains in this test.

结果：S4品系比CK1增产18.63%，比CK2增产29.99%，达到极显著水平，且挥发油和胡薄荷酮含量也高于其他参比品系和对照；S16品系与CK产量上无显著差异，抗病性比较强。

In the third chapter, the synthesis of5-[(1"S)-1",2"-diacetyl-oxyethyl]-1-phenylpyrazol-3-carboxaldehyde oxime(2) from5-[(1"S)-1",2"-diacetyl-oxyethyl]-1-phenylpyrazol-3-carboxaldehyde(1) and phenyl-hydrazine were introduced firstly. The synthesis of nine dioxapyrrolino[3,4-c]pyrazoles containing chiral pyrazolyl moiety(6a-i) from 2 with N-substituted-phenylmaleimide by nonmetal oxidant IBD-initiated intermolecular 1,3-dipolarcycloaddition was described in detail.

第三章首先以5-[(1′S)-1′，2′-二乙酰氧基乙基]-1-苯基吡唑-3-醛(1)和苯肼为原料，合成了化合物5-(1′S)-1′，2′-二乙酰氧基乙基1-1-苯基吡唑-3-醛-苯腙(2)；再用化合物(2)与N-取代苯基马来酰亚胺反应，利用无金属氧化剂—IBD引发1，3-偶极反应，发生分子间的环加成，合成了9种新型手性吡唑连二氧代吡咯啉并

In thesecond chapter, eleven 1,3,4-oxadizoles bearing chiral pyrazolyl moieties(4a-k) andfive triazolotriazines bearing chiral pyrazolyl moieties(5a-e) were obtained from5-[(1"S)-1",2-diacetyl-oxyethyl]-1-phenylpyrazol-3-carboxaldehyde(1) with arylcar-bohydrazide or (5-aryl-1,2,4-triazin-3-yl)hydrazine through an intramolecular1,3-dipolar cycloaddition in the presence of the non-metal oxidant IBD.

其中第二章介绍了以D-葡萄糖为原料制得的5-[(1′S)-1′，2′-二乙酰氧基乙基]-1-苯基吡唑-3-醛(1)分别与芳基甲酰肼、(5-取代苯基-1，2，4-三嗪-3-基)肼反应，在无金属氧化剂—IBD引发下，发生分子内1，3-偶极环加成，采用&一锅煮&法合成了11种含手性吡唑基的1，3，4-噁二唑类化合物(4a-k)和5种含手性吡唑基的三唑并三嗪类化合物(5a-e)。

So produced water how to reinject no damage into reservoir, is a very meaningful studying.

因此怎样实现污水的无伤害回注，是一个极有意义的研究课题。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Several studies suggest that making it harder for sex offenders to find a home or a job makes them more likely to reoffend.

多项研究证实，由于生活困难和居无定所，他们极有可能再次犯罪。

Scape usually longer than leaves, with 2-3 flowers each with 1bract, floral bract hooded, broad-ovate, as long as ovary, blunt and usually 3-lobed at apex; flower green-yellow, dorsal sepal white with yellow-green veins and green base, elliptic, 4-4.5cm long, short-pointed at apex, keeled on back, pubescent at base inside, petals very long, pendulous, twist, linear, 8.5-12cm long,6mm wide in middle; lip reversed helmet-shaped, longer than dorsal sepal, with claw almost as long as hood, side lobes inflexed, auricles large and nearly broad-triangular; staminode obcordate; stamens with thick and short filaments; ovary rostrate, 5.5cm long, glabrous.

花茎常长于叶，具2~3朵花，每花具一枚苞片，花苞片兜状，宽卵形，和子房等长，顶端钝而常3浅裂；花绿黄色，重萼片白色而有黄绿色的脉和绿色的基部，椭圆形，长4~5.5cm ，短尖，背面具龙骨状突起，基部内侧被短柔毛；花瓣极长，悬垂，扭曲，条形，长8.2~12cm ，中部宽约6mm ；唇瓣倒盔状较中萼片长，具爪，爪几乎和兜等长，具内折侧裂片，耳大而近阔三角形；退化雄蕊倒心形，上面基部具一个角状突起；雄蕊具短粗的花丝；子房具喙，长约5.5cm ，无毛。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。