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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Therefore it's necessary to contol the concentration of TW80 to use it in the remediation of pyrene contaminated soil by AMF and corn integrated systerm.4. The AMF community and the single species derived from rock oil-contaminated soil all can be combined with corn and promote the remidation of pyrene contaminated soil , the original AMF community has obvious advantages and behave best.The residual pyrene concentration of soil in the treatments with the mixed AMF community M4 (Glomus intraradices, Glomus mosseae, Glomus etunicatum and Acaulospora tuberculata) and corn integrated systerm got 0.12mg/kg,0.54mg/kg and 3.92mg/kg respectively in 1mg/kg, 10mg/kg and 50 mg/kg pyrene treatments,decreased 32.6%,58.6% and 42.2% respectively than that of the treatment of M1,decreased 28.1%,59.9% and 36.6% than that of M2(G.intradices), decreased 2.7%,55% and 23.5% than that of M3 (G.intradices + G.mossea).5.Large scale reproduce overall of AMF community which coming from rud oil-contaminated soil in the absence of the original conditions will change the quantiy,composition and structure of the AMF population and may has negative impact on it's function in the remedition of soil pyrene contamination.

因此,在植物与AMF联合修复土壤芘污染中使用TW80应该注意控制TW80的浓度。4、从石油污染土壤中扩繁出的AMF混合菌群及其中的单一菌种与玉米联合均能促进土壤芘污染的修复,原始混合菌群具有明显的种群优势,其与玉米联合修复土壤芘污染的效果最佳。1mg/kg、10mg/kg和50mg/kg芘处理土壤中,玉米接种混合菌种M4(Glomus intraradices, Glomus mosseae, Glomus etunicatum和Arcaulospora tuberculata)处理土壤的芘残留浓度分别为0.12mg/kg,0.54mg/kg和3.92mg/kg,分别比接种单一菌种M1处理降低了32.6%,58.6%和42.2%,比接种单一菌种M2(G.intradices)处理降低了28.1%,59.9%和36.6%,比接种复合菌种M3(G.intradices+G.mossea)处理降低了2.7%,55%和23.5%。5、从石油污染土壤中扩繁出的混合AMF菌群在无原始条件下的整体扩繁会改变菌群的组成及其结构,并会对菌群与玉米联合修复土壤芘污染的功能产生不利影响。

The stealth warplane for the microwave radar usually have complicated structures with the coating of wave absorption. On the base of calculation and modeling of the geometrical structure of the warplane in real size, the HF RCS of the air crafts under two different polarizations in different direction have been calculated with the FDTD. The result shows that the above mentioned stealth warplane have the obvious characterstics of frequency resonance, polarization and the relatively large RCS in HF Band 10-30dBm2, which can be used as the theoretical reference for selection of working frequency for sky wave OTH Radar and it also means that the sky wave OTH radar has good capability of anti-stealth targets quantitatively.

对微波雷达具有隐身作用的飞机,其形体较为复杂且表面涂敷有强吸波材料在对一种具有隐身几何外形结构实际尺寸飞机进行计算建模的基础上,文中采用FDTD技术得到了两种极化状态下该飞机无涂敷时不同方向上的短波雷达截面RCS值其结果显示了这种隐身几何外形飞机在短波波段有明显的频率谐振特性、极化特性和较大的RCS10-30dBm2,可为天波超视距雷达在选择工作频率时提供理论参考,并定量地说明天波超视距雷达具有较优的反隐身目标能力

Our results show that there were no significant difference in insect species and abundance between the massion pine forest and the slash pine forest.

通过对6种不同林分结构下的马尾松林和湿地松林内昆虫群落调查与多样性指数分析,表明2种松树内的昆虫种类和数量无显著差异,混交林中的昆虫群落的种类和数量比纯林多,尤其以捕食天敌类群的种类和数量更为明显。

"The dissociation between writing (a more common trigger for reflex epilepsy) and drawing emphasizes the presence of anatomically distinctive cognitive modules in the dominant and nondominant hemispheres."

通常能触发癫痫的书写活动和绘画引发的癫痫之间的无关联性揭示了:在占优势和非占优势的两个脑半球中,解剖结构上的思维模式明显不同。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是:藻体为单细胞或连成各式群体;细胞壁由硅质和果胶质组成,而且在结构上都是由上壳和下壳套合而成,纵断面观呈&肥皂盒&形;在上下壳的壳面上有花纹,花纹排列的方式主要分为两侧对称和辐射对称两大类,为分类上的最重要依据;能运动的种类在壳面都有壳缝,不能动的种类均无壳缝;色素体片状或带状,1个或2个,或为多个小盘状,黄绿色或黄褐色,含叶绿素a、c,还含有墨角藻黄素和硅甲黄素等光合色素;贮藏物质主要为油滴;繁殖方式主要为细胞分裂,硅藻的细胞分裂的明显特点是:每次细胞分裂所产生的2个子细胞中,仅有1个和母细胞等大,另1个则稍小。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。