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Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果:体外实验结果表明:一定浓度的硒作用HP后,HP菌落数量随着硒浓度的增加而减少,菌落颜色由灰白色变为砖红色,溶血现象随硒浓度的增加和作用时间的延长而逐渐消失,HP的形态由弯曲形变为球形或椭圆形,鞭毛及菌毛出现脱落现象;硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显;一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素,颜色随硒浓度增加而加深;一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长,减少重组VacA表达量,但不影响其空泡毒活性及编码基因序列;硒能增强重组VacA对胃癌细胞的抑制作用,使细胞分裂指数和集落形成率降低、S期细胞数量减少,G1期细胞总数增加;纯化后的VacA重组蛋白可被VacA抗体识别,具有良好的抗原性。

Results: Independent variables of year and hospital level, rather than area were associated with the increase of the two dependent variables. The two dependent variables positively correlated with each other.

结果:多元多层模型显示,自变量中的年份与医院级别对两因变量(医院仪器设备总值与业务收入)的增长值有影响,而地区对其无影响;两因变量间存在正相关关系(r=0.962)。

Results: Independent variables of year and hospital level, rather than area were associated with an increase of the two dependent variables. The two dependent variables positively correlated with each other.

结果:多元多层模型显示,自变量中的年份与医院级别对两因变量(医院仪器设备总值与业务收入)的增长值有影响,而地区对其无影响;两因变量间存在正相关关系(r=0.962)。

In the dissertation, meshless element free Galerkin method which utilizes moving least square is used for solving heat transfer problems. Lagrange multiplier method is used to deal with the essential boundary condition. Some parameters in element free Galerkin method, which influence the precision, are also discussed in the numerical simulation. The adaptive method of meshless method is also stated.

本文采用了基于移动最小二乘近似的无网格伽辽金法计算了热传导问题,采用拉格朗日乘子处理本征边界条件;讨论了无网格伽辽金法各个计算参数在数值模拟中对计算精度的影响;并对无网格的自适应方法进行了研究。

Different biases can result in the opposite phase shifts of the impedance with respect to the applied signal. Neutral gas pressure affects the magnitude of the impedance, but does not change the shape of the frequency response curve. In addition, the position of the grid inside the vacuum chamber has little effect on the impedance.

结果显示栅网上的直流偏压对阻抗的影响很大,不同偏压可以使阻抗的频率响应反位相,气压对阻抗的大小有明显影响,但一般不改变频率响应曲线的形状,栅网的位置对阻抗几乎无影响。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or P .01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or P .01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05, P 。01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05, P 。01);4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or 0.01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or 0.01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05或0.01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05或0.01);4种单体对过氧化氢损伤无显著的作用。

15 Mg .kg-1 given orally. The polyarthritic symptoms of the whole body in AA rats treated with Nime were markedly relieved.

而Nime对佐剂性关节炎大鼠低下的脾细胞增殖反应和腹腔巨噬细胞产生过高的IL-1活性却无明显影响,提示Nime无明显的免疫作用。

Significantly shortening of 〓C T〓s for orthorhombic crystals indicates that the thicknesses of crystals decrease during the course of stretching.

通过对不同拉伸比样品〓C T〓弛豫行为的研究发现,拉伸导致正交晶晶片厚度明显变薄,拉伸对于无定形相的高频分子运动几乎无影响。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。