无带的

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

The third-stage larvae were developed to fourth-stage larvae (the most optimal develop rate was 41%) when cultured in defined complete medium, further, cultured in defined incomplete medium, was examined no develop and a poorly survival rate. When the third-stage larvae were cultured in the defined complete medium under 37℃ and 5% CO2 in air, the larvae were began to develop to the fourth-stage larvae in cultured for 30 days, being enclosed within the sheaths of the third molts of life cycle.

广东住血线虫的第三期幼虫以MEM medium添加胺基酸、脂肪酸及碳水化合物的培养基在37℃含有5％ CO2的无菌培养箱中，培养至第21天已发育至第三期幼虫中期的阶段，第24天已发育至第三期幼虫末期的阶段，第30天开始出现带鞘的第四期幼虫，第36天则观察到虫体明显变长并蜕去外鞘及第三期幼虫特有的眼点消失的第四期幼虫。

In the design aspect, besides maintained the smooth line of cheongsam,clothing design masters retained its rigorous structure, do not have any nonessential accessories such as belt, button-fastening loop, appendix bag and so on; in craft aspect,abbreviating the shoulder part, useing the decoration flower and embroidery, all these things enhances the skin texture effect;structurally, not only adopting the pipa front, the pleasant lapel, the low lapel, the hich collar, the low collar,even on collar, but also involving its sisters art ,such as drawing, to make decorations for cheongsam.Those cheongsam on which drown flowers, birds, and makeups in Peking opera,are exotic and elegant. Graft the drawing onto the clothing design and improve the originally structure which is insufficiently comfortable,the new style cheongsam become a marvellous achivement on the international clothing stage Moreover, the production technology innovation, the technical process improvement, the new material use, all of these has poured the new blood into the Chinese dress development.

在款式方面，除了保持旗袍流畅的线条外，服装大师们在设计剪裁时，保留旗袍结构严谨，线条流畅，没有任何不必要的带，袢，袋等附件的特点；在工艺上采用收肩，饰花，穿珠片，刺绣等手法，加强肌理效果，在构造上，采用琵琶襟，如意襟，低襟，高领，低领，无领等，同时又把绘画等姊妹艺术也作为旗袍装饰的手段，那些画有花，鸟，京剧脸谱等图案的旗袍，不仅设计新巧，而且风格典雅，富有韵味，把服装和绘画融为一体，打破了原来穿着不够舒服的构造，使之一展新容，成为国际服装舞台上的奇葩。

Finally there is this great new BMS set that includes a sensorless 15T brushless motor with delta winding and a suitable speed controller, supplied in a handy case, all for a cost effective price and great for those people who want to upgrade from the standard equipment that came with their RTR car.

最后有这个伟大的新的管理系统设置，其中包括一个传感器15T无刷电机绕组与三角洲和一个合适的速度控制器，提供了方便的情况下，所有的具有成本效益的价格和巨大的人谁想要升级从标准的设备，带着他们的RTR车。

The ferroelectrics with photoelectronic, acoustooptic, and nonlinear optical effects are good candidate for constructing optical switches, power splitters, couplers, filters, frequency doublers, and integrated photonic devices. Especially, the newly developed fabrication technology for ferroelectric-based photonic crystals renders a perspective application of ferroelectric materials in photonic band-gap engineering.

具有热释电效应的铁电物质是制作新一代非致冷红外探测器的理想材料；利用铁电体的电光、声光和非线性光学效应，可构建光开关、功分器、耦合器、滤波器、倍频器等无源光学元器件或集成光子器件；特别是近年来发展的铁电光子晶体制备技术对推动和拓展铁电氧化物在光子带隙工程领域的应用带来了新的希望。

In the recent years, the company has successively developed the fully servo control extra-fine metallic wire drawing machine without tension bar, stainless steel fine wire drawing and annealing machine, the true meaning constant tension and constant line rate and fully-automatic single-head and single-control annealing wire coiling machine, fully-automatic active wire laying device and inductive and constant annealing extra-fine copper wire drawing machine and other new products, which have basically solved the defects of the finished product wires manufactured by ordinary equipment that tension inequality, disordered wire arranging, incapability for anti-drawing intensity and extension rate control and low finished product rate, etc and have improved the product quality to a higher grade.

近年来，本公司相继开发出全伺服控制无张力杆的贵金属极细线拉线机，不锈钢微丝拉丝退火一体机，真正意义上的恒张力，恒线速，全自动的单头单控退火线绕线机，全自动主动放线装置以及带感应式连续退火的极细铜线拉线机等新产品，根本解决了普通设备生产出的成品线内外松紧不一，排线不整齐，抗拉强度和延伸率无法控制，成品率低等缺陷，使产品质量更上了一个档次，设备的外观，制造精度和技术性能可以与进口设备相媲美，完全能够满足客户国外订单的高品质要求。

The Neo-Confucianism of Taiwan and Hongkong\'s aesthetic study combines the artistic spirit of Confucianism and Taoism, introjects the aesthetics of China and the west, and excavates the modern merits of traditional aesthetic, which are important problems of the modern Chinese aesthetics. Therefore, it is very helpful to the modern Chinese aesthetic and the comparative poetics that we scan the Neo-Confucianism of Taiwan and Hongkong\'s problems again and pursue the evolution of these problems in a cross-cultural and cross-subject view.

由于台港新儒家的美学研究兼有会通儒道艺术精神、融合中西美学思想，及发掘传统美学之当代价值等的层面，这些无一不是中国当代美学的重大课题，因此带着问题意识用跨文化、跨学科的视野重新审视他们探讨过的问题并追踪问题的延伸，将对中国当代美学的建构和比较诗学的研究都有着重要的启发意义。

The effect of iner- tia weight factor on performance of PSO is discussed.The results show that the particle swarm optimiza- tion algorithm with nonlinear inertia weight factor is suitable to solve multivariahle optimum design prob- lem...

结果表明，带动态非线性惯性因子的微粒群算法对求解多变量的干燥优化设计问题具有方法简单、所需微粒群规模小、收敛速度快等特点；采用部分废气循环并进行优化设计对干燥系统的节能具有十分重要的意义，对湿空气出口温度和废气循环比进行优化设计，其年总费用比无废气循环的常规设计节省18.2%，比循环比为0.2时的常规设计节省12.6%。

On the proposals of certain ministers at the Committee for Propagation of the Gospel Cromwell, our chief of men, who through a cloud Not of war only, but detractions rude, Guided by faith and matchless fortitude, To peace and truth thy glorious way hast plough'd, And on the neck of crowned Fortune proud Hast rear'd God's trophies, and his work pursu'd, While Darwen stream with blood of Scots imbru'd, And Dunbar field, resounds thy praises loud, And Worcester's laureate wreath; yet much remains To conquer still: peace hath her victories No less renown'd than war.

赠克伦威尔将军克伦威尔将军，我们的领袖呵，看你信仰虔诚，刚强无此，终于冲过了战云与流言的暗影，在坚苦卓绝中，取得了和平与真理；你能控制住骄盈王室的气运，为神展开了事功，取得了胜利；苏格兰鲜血染红的达汶流水，敦坝的战场，渥斯特光荣的战役，都在高声歌唱你。但你的工作还不曾完结；平时和战时同样能创造不朽的事业：新敌人又出现，想把政治的锁链给心灵带上。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了