无孔的

3H］-TdR incorporation rate was used as an index of cell proliferation. Additionally, we investigated whether the ASMC proliferative responses to SP were modulated by preincubation for 2 h with GR71251, a specific antagonist of NK-1 receptor, and neomycin, a phospholipase C inhibitor, and nitrendipine, a blockader of Ca2+ channel, respectively.

细胞增殖实验研究：将平滑肌细胞以1×103/孔接种在96孔板内，含10% FCS-DMEM培养24 h，吸去上清，以无血清DMEM洗涤2次，加入该培养基培养48 h，同步化之后以无血清培养基继续培养48 h或分别加入含SP(10-7～10-5 mol/L)和NK-1受体激动剂［Sar9，Met(O2)11］-SP(10-7～10-5 mol/L)的无血清DMEM培养相同时间。

The rain is knockin at my heart and I can't see the sun no more I can't live without you my sun if I'm ever gonna get back picture this You got a kid in a room his hands sweatin and shakin while he's lacin his shoes the excitement's buildin up for what's comin up soon To get there and do what he was born to do He's listenin to the beats He's trying to get in the zone He's gotta take control and make every moment his own just a little bit tired he is a little bit scared but he's so focused he won't show it when he's finally there Cuz now he's out on the floor And he's catchin the pass slashin to the glass, movin so fast, right to the hole, they don't know how to stop him and he keeps on droppin 2's and 3's by the second and the crowd is rockin so they knock him knock him down to the ground before he's crowned as the best He takes a deep breath before he gets up and looks around Everything's in slow motion The crowd is an ocean Only one minute left The rain is knockin at my heart and I can't see the sun no more I can't live without you me sun if I'm ever gonna get back, get back, get back The rain is knockin at my heart and I can't see the sun no more Ican't lice without you my sunif I'm ever gonna get back Imagine A little girl in the stands the kid's her older brother and she's his number one fan He's always lookin out for her and she looks up to him and she wants nothin more than for her brother to win But the truth is that there's someone on the other team Someone who stole her heart Someone who holds her dreams They kiss for the 1st time just the other evening Her brother's 23 but her lover's number 16 The drama is set the whole scene is intense she doesn't know which one to cheer for and she's gettin upset 23 on the chase cuz they're at each other's necks and what happens next Her boyfriend steals the ball and checks the kid in the chest 16 on the break Brother goes in the air with brother up in his face They come crashing to the dirt Tears about to burst When the game is over who she gonna run to first, yo The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 有时感觉阻碍太多但我还是为了我的梦想而奋斗 Nothin's gonna stop me Ey yo this game of life is one big struggle We gotta hustle just to make it everyday to fight the fears we facin and grab those dreams we chasin cuz there ain't no competition when we rise to the occasion c'mon The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 注:IN可以看作是ING如FACIN可以看作facing

雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来此图片你有一个孩子在一个房间里他的手sweatin和沙金，而他的拉欣他的鞋兴奋的buildin了什么的，很快就要来要到那里做什么，他出生做他listenin的节拍他试图在该地区获得他得采取控制和利用自己的每一刻只是有点累了他有点害怕但他是如此专注，他将不显示它的时候，他终于有现在的Cuz他在地上他的捉通行证 slashin的玻璃，居无定得那么快，有权向孔，他们不知道如何阻止他他不断droppin 2的和3的由第二摇滚和人群所以他们会把他会把他撞倒在地之前，他的加冕为最佳他深吸了一口气，才起身环顾四周一切都在慢动作人群是一个海洋仅1分钟雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来，回来，回来雨是敲入我的心我看不到太阳不再没有你我的sunif Ican't虱子我曾经去买回来想像看台上的一个小女孩这个孩子是她的哥哥，她是他的头号球迷他总是在找谁了她和她抬起头来他和她想没什么比她的哥哥更赢得但是，事实是，对其他团队的人有人谁偷了她的心有人谁拥有她的梦想他们只是亲吻对方的第一时间为晚上她的弟弟，但她的爱人23的16号该剧设置整个场面激烈她不知道哪一个加油，她的gettin不高兴 23大通他们的Cuz在对方的脖子和接下来会发生什么她的男朋友抢断球，并检查孩子的胸部 16个突破哥哥和弟弟去他的脸在空中他们来到崩溃的污垢即将爆裂泪当比赛结束谁，她要去跑第一，溜溜雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来有时感觉阻碍太多但我还是为了我的梦想而奋斗没办法，拢不惊安永哟这样的生活游戏是一个大斗争我们得喧嚣这只是为了日常战斗的恐惧，我们都会面临的两个并抓住那些梦想，我们查辛没有的Cuz没有竞争当我们挺身而出来吧雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明，枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成，是位于背部的一个囊状结构，它由两部分构成，前部的方形囊状体和后部的三角形囊状体，二者之间有一突起的脊；雌蛾静止时，腺体随第八和第九腹节一起嵌缩于第七腹节内，求偶时，腹部末端外伸，腺体细胞表皮外露，释放性信息素；腺体细胞呈方形，其表面覆盖几丁质表皮，表皮顶端无孔；细胞核很大，呈椭圆形，由双层核膜包被，位于细胞中下部，内有丰富的染色质；细胞质内有丰富的内质网，其内还有大量的大小不等的脂肪滴，以及线粒体和溶酶体等细胞器。

Plants apparently almost stemless with a rosette of leaves flat on ground and a very dense central cluster of flowering branches; nutlets with glochids

植株表面上几乎无茎的具一在地和一花枝的非常紧密的中心的群上平的叶的莲座丛；有钩毛的小坚果 29 Microula tibetica 西藏微孔草

Calculates the coordinates of the spindle and drive shaft and checking the center distance, determined department spindle must use dislodges the gear.

根据柴油机齿轮室盖的结构特点、加工部位、尺寸精度、表面粗糙度及生产率等要求，确定该机床为单工位卧式组合机床；为确保加工精度，采用&一面两销&的定位方案；为实现无级调速，安全可靠，选择液压滑台；根据零件的大小及被加工孔的位置确定主轴箱的轮廓尺寸；由加工工艺选择滚珠轴承主轴，通过计算扭矩确定主轴和传动轴的直径；齿轮的模数是通过类比法确定；齿轮齿数和中间传动轴的位置是由&计算、作图和多次试凑&相结合的办法确定；计算主轴、传动轴的坐标并进行中心距验算，确定部分轴上需采用变位齿轮；轴上的齿轮套、键等零件按轴号选取相应的标准件。

Second and sometimes 1st year's branchlets with conspicuous lenticels; leaf blade abaxially glabrous except grayish pubescent to densely slightly crisped villous along veins

第二和有时加明显皮孔的1年的小枝；叶片背面除对浓密稍皱波状的具长柔毛沿脉的浅灰色的短柔毛之外无毛 21 H。 xanthoneura 挂苦绣球

Screws, bolts, or nuts without washers must be loaded against a test plate with a minimum diameter clearance hole equal to the nominal thread size plus 0.50mm for fasteners M6 (1/4 inch) and smaller, and plus 1.0mm for larger diameters.

无垫圈的螺钉、螺栓、或螺母应被装夹到具有较小孔径的通孔的试验圆板上进行试验，对于≤M6 (1/4 inch)的紧固件，试验圆板的孔径为公称螺纹直径加上0.50mm，对于＞M6 (1/4 inch)的紧固件，试验圆板的孔径为公称螺纹直径加上1.0mm 。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

First, by the allocation of irrigation water directly into the ditch outlet Xiaqi, achieve no water loss, greatly improve the utilization of water; are two irrigation systems, replace the pipe with water furrow, not square, and the right to re-use, reducing the irrigation investment; Three is the use of the gate with the outlet opening to adjust a constant and accurate control of flow into the trench plot, for different soil and different groove specifications Xiaqi can greatly improve irrigation uniformity; four gates are porous pipe irrigation system for the flow tubes, and the blind to cross the flow tube as compared to the wall can reduce the work under stress, and reduce the damage to the opportunity to prolong the service life of thin-walled tube; are five gates to facilitate the installation of irrigation systems, pipes, and, upon request, to fight孔安outlet assembly to ensure the accuracy of positioning with the outlet.

一是灌溉水经配水口直接进入沟畦，实现无输水损失，极大地提高了水的利用率；二是管灌溉系统代替配水垄沟，不占地，且右重复使用，降低了灌溉投资；三是利用配水口的闸板开度来调节准确恒定地控制进入沟畦的流量，适用于不同土质和规格不同的沟畦，可大大提高灌水均匀度；四是闸管灌溉系统为多孔出流管，与等过流量的盲管相比，可降低管壁承受的工作压力，并减少受损机会，从而延长薄壁管的使用寿命；五是闸管灌溉系统安装方便，并可根据要求打孔安装配水口，确保配水口定位的准确性。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！