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On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明:早孕胎盘来源的细胞滋养层细胞在血清诱导下,首先发生聚集,并通过桥粒和细胞骨架使相邻的细胞膜紧密接触;之后细胞膜发生片段断裂,形成融合孔样结构;细胞间桥粒消失,最终形成多核的合体细胞;免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂(amitosis即细胞核分裂而细胞质不分裂)实现的。

A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥,放入玻璃管内,密封得搅拌磁子;配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液,加入有机碱得预键合溶液,将搅拌磁子浸入预键合溶液,在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质;将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中,将预键合后的搅拌磁子放入容器,密封后置于水浴中,反应后除去容器,取出涂有聚合物的搅拌磁子,置于提取器中,以溶剂提取,或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出,得产物。

Giving first place to the Inarticulata as the genus and species of the Articulata fossil are monotonous , the fossils include Obolidae Lingulella、Lingulepis, Acrotretidae Acrothele、Orthacrothele, Kutorginidae Kutorgina,Daterinidae Paterina and Iphidella, Micromitridae Micromitra, and Nisusidae Nisusia of the Articulata fossil.

以无铰纲为主,有绞钢化石属种单调,包括圆货贝科的Lingulella、Lingulepis,乳孔贝科的Acrothele、Orthacrothele,顾脱科的Kutorgina;神父贝科Paterina及Iphidella;小帽贝科的Micromitra,及具铰纲艾苏贝科的Nisusia。

After SBF solution cultivation for some days, the Ca/P coating was very easily deposited on the porous surface that was constituted of cystiform polygon made up with irregular nanometer circles like honeycomb. The energy spectrum showed that n/n atom ratio is about 1.61:1 which is very similar to that of HA and human bone. The active surface of the porous titanium after acid-alkali treatment and SBF solution condition which calcium-phosphorus ions was supersaturated induced the formation of Ca/P deposition layer.

SBF溶液培养后发现沉积层由蜂巢状多边形胞状物组成,这些胞状物是由网状纳米环无规则排列构成,能谱测定钙、磷元素原子比n/n约为1.61∶1,与HA和人骨中的钙磷原子比很接近;通过对沉积机理的分析,认为微孔钛的活性表面和SBF溶液过饱和离子浓度的环境造成了液相中钙磷盐的沉积。

In the Plain ,Wu Manling ' s female consciousness is submerged in the political ideology,while San Ya and Kong Suzhen ' s female consciousness are hidden in class awareness. Wan Yi in Narrating keeps her female awareness in national consciousness,Yu Mi, In Yumi, has her sororal sense in the form of power awareness. In Qingyi, Xiu Yanqiu ' s female awareness appears in the sense of fatalism. Female conscousness in most of his novels seem to become a collective conscious.

平原》中吴曼玲的女性意识淹没于政治意识,三丫、孔素珍女性意识淹没于阶级意识,《叙事》中婉仪的女性意识淹没于民族意识,《玉米》中的玉米姐妹的女性意识淹没于权力意识,《青衣》中筱燕秋的女性意识淹没于宿命意识,其很多小说女性意识似乎成为一种集体无意识。

Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6/ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片,分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化,即方法1先用含bFGF的培养基进行预诱导,之后再用含叔丁基对羟基茴香醚,和二甲亚砜的无血清培养基进行诱导;方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导,然后不同浓度的上述物质进行正式诱导。

The closed-cell aluminum foam was fabricated by direct foaming in melt. Through sampling aluminum foam in different stages the cross-section of them were observed and analyzed. It was explained how TiH_2 was decomposed after entered into the melt, how initial bubbles were formed and what was the state that generated bubbles and undecomposed TiH_2 lay in.

以熔体直接发泡法制备闭孔泡沫铝材实验为基础,通过获得不同实验阶段的泡沫铝样品,以及对实验样品切面或断面进行观察和分析,描述了在熔体发泡法制造泡沫铝过程中TiH2加入熔体后的分解过程,原始气泡的形成方式以及产生的气泡和未分解TiH2的存在状态;解释了气泡进一步长大的原因和未分解的TiH2如何释放气体;表述了气泡的合并和无泡层的形成。

This paper made the value simulation about the flow filed around the net and non-net dumpsite, referring to dusting pattern to establish he source strong simulation pattern of material pilesraising the dust to discharge in the coal dump site that the US environmental protection bureauRecommended.

本文使用标准κ-ε模型对有网堆场周围的流场和无网堆场周围的流场进行了数值模拟,参考美国环境保护局推荐煤堆场起尘模式建立料堆扬尘排放源强模拟模式,给出了不同开孔率的防风网对静态煤堆场的抑尘率,为防风网的设置提供技术参数。

Results In healthy subjects, L2-L5 nerves were displayed clearly by MRN, with a round or ellipse ganglion of 3-6 mm in diameter, and smooth stick-like nerve root and trunk, 2-5 mm in diameter. The signal intensity was higher in ganglion than nerve root or trunk. 40% of nerve root sheath was surrounded by small amount of cerebrospinal fluid, Femoral nerve was displayed in all subjects, while only 83.3% of obturator nerve and 43.3% of lumbosacral trunk were manifested. Lamellar blurred hyper-signal intensity was seen around 80.9% of lumbar plexus in GBS, but none in CIDP.

结果 正常组MRN可清晰显示腰2~5神经的走行,神经节呈圆或椭圆形膨大,大小约为3~6mm,神经根及干呈条状、边缘光滑,大小约为2~5mm、神经节信号高于神经根和干,40%的神经根鞘有少量脑脊液,股神经、闭孔神经、腰骶干的显示率分别为100%、83.3%、43.3%。80.9%的GBS腰丛神经周围有片状模糊高信号,神经根和节大小和信号无明显改变,25%神经干增粗,50%的神经干信号增高,边缘模糊。

According to the spot experiment result of bolt anchor-hold in the condition of draining water in the roof on haulage roadway, 11201 coalface, Chengjiao coalmine, the test facility was designed independently and the influence of the anchor-hold of the resin bolt by the draining water in the drill hole was studied.

结合城郊煤矿11201工作面运输巷顶板淋水情况下锚杆锚固力现场测试结果,自行设计了实验装置,研究了钻孔淋水对树脂锚杆锚固力的影响。在现场测试中,当钻孔的淋水量小于128mL/min时,树脂锚杆的锚固力保持不变;当淋水量大于583mL/min时,树脂锚杆的锚固力比无淋水时的锚固力下降35%以上。沿巷道轴向布置卸水孔,采用集中排放,同时加大锚杆支护的密度可以改善顶板淋水时锚杆支护的效果

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