无子叶的

The caulicle and the root of Rh Sachalinensis The induction of callus from the cotyledon is easiest.

利用高山红景天无菌苗的子叶、幼叶、幼茎和根成功诱导出了7种愈伤组织。

0Mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.

结果表明：不同黄瓜品种直接不定芽数量诱导存在显著差异，但长度诱导无显著差异；6-苄氨基嘌呤对直接不定芽数量和长度的诱导作用显著，其中4.0mg/L为数量诱导适宜浓度，其出芽率和每外植体出芽数达到最高，分别为100.0%和11.1，0.5mg/L为长度诱导适宜浓度，其平均芽长12.0mm；外植体的大小对直接不定芽数量和长度均具显著影响，随着子叶的增大诱导直接不定芽的数量和长度增加，其中单片完整子叶为最适宜大小，其出芽率、每外植体出芽数和平均芽长分别达到100%、6.7和17.1mm；硝酸银也对直接不定芽数量诱导影响显著，其中2.0mg/L为适宜浓度，出芽率和每外植体出芽数分别达到97.2%和4.2，但对芽长有抑制作用。

The related factors of tissue culture were studied by using cotyledon of 7B7, self-hybrided Helianthus annuus L. as inducible material and MS as basic medium.

以油葵自交种7B7的无菌苗子叶为诱导不定芽材料，MS培养基为基本培养基，研究油葵子叶组织培养有关影响因子。

The morphology of M2 and M3 was observed in hothouse and mutants on cotyledon and root of seedling, or embryo of seed were identified. The result showed that there were 8 types of mutative treats on cotyledon, including light-yellow cotyledon, yellowish cotyledon, trump-shaped cotyledon, multilobed cotyledon, non-cotyledon, single-cotyledon, tricotyledon and quadrcotyledon.

M_2和M_3的水培和形态学观察的结果发现，子叶性状出现浅色、黄化、喇叭形、多耳突、无子叶、单子叶、三子叶、四子叶等8种变异类型；根系性状出现短小根、发达根、无侧根和弱向地性等4种变异类型；种胚性状中发现了多胚变异。

Further examinations of cell size, trichome distribution and meristematic activity failed to find any differences between cotyledons of European species. All observations showed that both cotyledons of European Gesneriaceae species ceased to grow in early stages and thus no prolonged meristematic activity could be observed.

进一步检测子叶的细胞大小、毛状物的分布、及分生组织活性检测均显示欧洲苦苣苔科成员的两片子叶在发育早期已停止生长，两片子叶间也无形态上明显的差异，同时也无法观察到持续的分生组织活性刺激子叶持续生长。

Fruit a usually solitary follicle, sessile or stipitate, dehiscing usually along adaxial suture, sometimes along abaxial one, very rarely circumscissile at base, sometimes indehiscent.

果通常为单生的蓇葖果种子1(或者2)，直立，通常具假种皮，很少无假种皮；种皮厚假种皮有色，肉质；胚乳宿存或无；胚直立；在无胚乳的种子里子叶厚并且肉质，在有胚乳的种子里子叶薄。

Plantlet regeneration from cotyledon of Citrullus lanatus cv. Zhangkang No. 4 was studied. The results showed that aseptic seedlings should be cultured in dark for 3d, then exposed to a photoperiod of 16/8h/d for 3d. The callus induction rate was higher in cotyledons placed facing downwards to the medium than in those facing upwards. The highest induction rate occurred in MS medium containing 6-BA (2.0mg/L), kinetin (1.0mg/L)and GA3 (1.0mg/L). The induction initiated in dark and took 7 days, while callus growth and differentiation proceeding for 7 days under 16/8h light-dark cycles. A highest rate of embryogenic callus was obtained after 3 successive subcultures.

以无子西瓜郑抗4号无菌苗子叶为外植体，进行了体细胞胚发生及植株再生的研究，结果表明，无菌苗应先进行3d暗培养，然后采取光照16h/d和黑暗8h/d培养3d；将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式；子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L；诱导需要在黑暗条件下启动，进行7d暗培养，而生长分化于光照16h/d和黑暗8h/d条件下培养7d；继代3次得到的胚性愈伤组织最多；最适生根培养基配方为1/2MS+IBA0.3mg/L。

Multiple comparisons suggested that cotyledon with petiole or 1/4 piece of cotyledonary laminae from 7 days old aseptic seedling had much higher shoot regeneration frequency, reaching to over 90%.

多重比较结果表明，以7d苗龄无菌苗的带柄子叶或1/4子叶片为外植体较好，出芽率较高，达90%以上。

Effect of 6-BA on regeneration of cotyledonary node including germination of seedlings, multiple shoots induction and shoots elongation from soybean cultivars "heinong 35" were studied.

以大豆品种黑农35的子叶节为材料，研究6-BA对子叶节再生三个阶段即无菌苗萌发、丛生芽诱导、丛生芽伸长的影响。

Seeds small, obovate, angular and wingless or planoconvex with a narrow marginal wing; testa crustaceous, shiny; embryo small, straight; cotyledons subrounded, clasping, embedded in copious fleshy endosperm.

种子小，倒卵形，具角和无翼的或平凸具一个狭窄的边缘的翅；种皮壳质，发亮；胚小，直；子叶近圆形，抱茎，嵌入大量肉质胚乳中。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。