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These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设,我们设计了10种培养条件,在自行设计的无血清培养基中添加不同的细胞分化诱导物,包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等,分离孕10周龄人胎肝细胞进行原代培养,观测肝细胞形态及功能指标变化,并定时给予HBV感染,观察能否在体外诱导胎肝细胞出现HBV易感性;为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA),我们改进了现有的PCR检测法。

RESULTS: Both resting B cells and T cells did not express PD-L1 on their surface, however PD-L1 expression was significantly up-regulated on the surface of the activated B cells after 6 h stimulation with LPS or pokeweed mitogen, and the percentages of B cells that expressed PD-L1 reached a plateau at 24 h, which were (46.26±10.71)% with LPS and (43.67±6.14)% with PWM stimulation, respectively. No markedly change of PD-L1 expression on the surface of the activated T cells after stimulation with LPS was observed, but upregulation of PD-L1 expression was observed when stimulation with PWM.

结果: 静息B细胞和T细胞表面不表达PD-L1,LPS和PWM刺激6 h后表达PD-L1的B细胞百分率均显著高于静息B细胞,24 h达到最高,分别为(46.26±10.71)%和(43.67±6.14)%,之后随时间延长而降低;表达PD-L1的T细胞百分率在LPS刺激下无显著变化,但PWM刺激6 h后百分率明显高于静息条件,24 h达到最高,为(25.42±9.23)%,之后下降。

However, the cell wall cultured at high concentration of Cu2+(10~200 mg/L) was clearly thickened and became multiple layers, and the pigment decreased and the pyrenoid became one or disappeared. Low concentration of Cu2+(0.01 mg/L, 0.1 mg/L) did not affect the growth of Chlorococcum sp., but the growth became slow and even negative beyond the concentration of 0.1 mg/L.

与对照BG11培养的绿球藻比较,0.01~1 mg/L Cu2+浓度下培养的绿球藻,细胞壁无明显增厚,色素没有多大变化,但蛋白核由一个变为多个;而在高浓度(10~200 mg/L Cu2+)下,细胞壁明显增厚为多层,色素减少,蛋白核减少并回复到1个或消失。

Statistical significance can not be found between 24hours and 48 hours.. Under an inverted microscope , we can observed the cells adherent, fullness, polygon in control group, the cells amout sparse, suspended, quasi-round, and no refractional nature in experiment groups, the highter the concentration is, the greater themorphological change of cells will become.

形态学分析,在倒置显微镜下可见,经药物作用后MCF-7细胞数目减少,细胞形态由原来的贴壁良好、饱满、多角形、梭形变为类圆形、悬浮、颜色变暗、无折光性,且随药物浓度的增加细胞形态变化越大。2。

The visual acuity and light sensitivity were altered by TTT in the eyes with absolute scotoma.

所有被检出有绝对暗点的患眼治疗后视力均无明显变化,视网膜的光敏度不变。

Results Absolute scotoma appeared in 7 eyes and relative scotoma in 6 eyes before TTT. Among the 6 eyes with relative scotoma, the visual acuity was improved over 2 lines in 4 eyes after TTT, descended 1 line in I eye and unchanged in 1 eye. The light sensitivity of the irradiated retina was increased in 5 eyes and decreased in 1 eye.

治疗后与治疗前相比较,6眼TTT前检出相对暗点的患眼中,治疗后视力提高者4眼,1眼视力下降,1眼视力无明显变化;6眼中5眼TTT治疗后激光照射处视网膜的光敏度提高,1眼降低。

This study uses seventy branches of case bank as basic samples in 6 years through 1997 to 1999 and 2003 to 2005 representing the periods before and after privatization respectively and employs an evaluation approach of operational efficiency-Data Envelopment Analysisto measure the operational efficiency of branches and justify the reasons of inefficiency. At the same time, we suggest with some directions and magnitude of improvement by using the method of slack variable analysis.

本研究以该银行70家分行,於民国86至88年及92至94年,改制民营前后六年资料为样本,运用经营效率评估方法--资料包络分析法,先探讨各分行之经营效率,了解无效率之原因,并透过差额变数分析,提供其改善的方向与幅度,同时探讨该银行民营化前后各分行经营效率的变化。

Expression of NIS gene and protein in breast cancer could be regulated by many kinds of factors including steroids, thyrotropin, triiodothyronine, sodium iodide, TNF-α, IFN-γ, IL-6, retinoic acid, prolactin, and leptin.

而且,RA和瘦素使乳腺癌细胞的摄碘功能明显增强,PRL培养的细胞摄碘功能并无明显变化。

Gridless smooth particle hydrodynamic numerical technique of AUTODYN-2D hydrocode is used to simulate the impact behavior of spherical and cylindrical space debris on-orbit impacted at a velocity of 10km/s.

采用AUTODYN-2D无网格SPH数值技术,对碰撞速度10km/s下的球形及柱状空间碎片在轨碰撞超高速作用行为进行数值模拟,给出了不同节点处的速度变化规律。

However,there was no significant difference of lung function tested by spirometry.

予以强的松10 mg/d治疗后病人的生活质量(饮食、体重、呼吸困难的主观感觉、6 min平地步行距离)明显改善,急性发作次数减少,肺功能无明显变化。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。