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Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

GA had no effect on SMA constriction induced by NE. The reactivity of SMA rings to myricetin was increased at 0, 0.5, 1 hour and 2 hours after shock, and it reached the peak at 2 hours, then it significantly decreased at 3 hours and 4 hours after shock.

结果 SMA血管环对NE的反应性表现为:休克0 h和0.5 h组明显高于正常对照组和其他休克时间点组,休克1 h和2 h组明显低于正常对照组和休克0 h和0.5 h组;18α-GA对NE诱导的SMA血管环收缩反应性无明显影响。

With thermophilic alcohol active dry yeast as fermenting microorganism, continuous fermentation experiment was carried out.

建立了适合无溢流的硅橡胶乙醇连续发酵膜生物反应反应器系统的细胞反应动力学模型。

T show evidence of toxicity between the verapamil treated animals and control animals.conclusionsubconjunctival verapamil decreased the incidence of tractional retinal detachment due to traumatic proliferative vetreoretinopathy in this rabbit model.verapamil at the dose used in this model has no evident toxicity on rabbit eyes.further studies are needed to determine the doseresponse and efficacy of the drug.

结果在模型制备后第35天,治疗组18只眼发生牵引性视网膜脱离,占56.3%,对照组26只眼发生,占81.3%(χ2=4.655,p=0.031);玻璃体视网膜增生程度治疗组较对照组明显减轻(秩和检验,u=3.5419,p<0.001);未发现ver对角膜上皮有明显毒性反应。结论结膜下注射ver能有效抑制外伤性pvr的发展,减少牵引性视网膜脱离的发生率;ver在实验所用剂量对角膜上皮无明显毒性反应。

The analysis results of FTIR , SEM, X-ray Diffraction and TG-DSC proved that,the existence of graft copolymerization between cassava starch and acrylamide was occured;the structures of cassava were destroyed during the copolymerizing; the structures of the copolymer were largely amorphous; within the range of 700℃,the thermal degradation process of the St-g-PAM was divided into three-steps, which was more complex than that of cassava.

FTIR、SEM、X射线衍射、TG-DSC分析结果表明木薯淀粉与丙烯酰胺已发生接枝共聚反应;木薯淀粉在接枝共聚反应中,结构被破坏,接枝共聚物的结构基本上为无定型结构;在700℃温度范围内,木薯淀粉丙烯酰胺接枝共聚物的热降解为三步降解历程,比木薯淀粉复杂。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明,BSA-SM人工抗原分子结合比为1∶25;筛选出3F9-C4敏感特异的杂交瘤细胞1株,间接ELISA测定细胞培养上清,效价为1∶3.2×102,同种型为IgG2a/κ;腹水的亲和常数为8.4×1011L/mol;IC50为8.99μg/L,与双氢链霉素交叉反应为109.6%,与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明,BSA-SM人工抗原分子结合比为1:25;筛选出3F9-C4敏感特异的杂交瘤细胞l株,间接ELISA 刚定细胞培养上清,效价为l:3.2×10^2,同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol;IC50为8.99μg/L,与双氢链霉素交叉反应为109.6%,与其他SM结构相似物和功能近似物无交叉反应性。

A facile one-step method waspresented for the synthesis of N-fluoroalkanesulfonyl amidines 〓 inmoderate to good yield from the reaction of azides, ketones or aldehydes, andsecondary amines at room temperature.

在和无官能团取代的烯胺化合物的反应中,它发生了烷基迁移过程,而且利用该反应我们实现了由酮或醛,二级胺和叠氮一锅法合成脒类化合物〓。

The positive seroconversion rates of neutralizing antibody were 100%(n=27 GMT 2.89 IU/ml) and 100%(n=30, GMT 5.29 IU/ml) respectively by pre-exposure and post-exposure schedules.

结果 全部受试者仅显示12%的轻度副反应,无中强度副反应;不管以暴露前或暴露后方案接种,全程注射后的中和抗体阳转率均为100%。

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