无双的

The analysis of the mutants of amrA and amkA showed that the production of rifamycin SV had decreased about 10%-20%.

用双交换法在地中海拟无枝菌酸菌U32染色体上插入阿泊拉霉素抗性基因，成功地构建了了amrA，amkA和amrB基因的突变株，其中amrA，amkA基因突变株的菌丝形态与野生型U32之间有较明显的差别，在菌丝的末端形成卷曲或弯曲。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

Seeds uniseriate or rarely biseriate, wingless, globose or rarely oblong, plump or rarely slightly flattened; seed coat reticulate, mucilaginous or not when wetted; cotyledons conduplicate.

双列的单列的种子或很少，无翅，或很少长圆形，肥厚或很少稍扁平；当被弄湿时，不的种皮网状，黏的或；子叶对折。

Objective:By histochemical method, the four biotinylated lectins (DBA, SBA, WGA, UEA 1) were used to study changos in glycoconjugate expression on the surface of endometrium of 28 cases of RSA without obvious reasons in secretary phase, at the same time, endometrial biopsies were taken for histomorphologic evolution.

本文通过生物素分别标记的双花藕豆凝集素、大豆凝集素、麦胚凝集素及荆豆凝集素（UEA－1）与子宫内膜的反应，应用S－P法研究28例无明显原因反复流产的分泌期子宫内膜糖复合物的变化及与其组织形态学分期的相关性。

Based on 3D steady and incompressible N-S equation and k-ε turbulent model, the aerodynamic performance of three different design options of 160 km/h container flat wagon was analyzed and compared using finite volume method. The three design options are as follows: the first design option has no headwall and the side surface of side beam is flat; the second and the third both have headwall and high side beam, and the side surface of side beam of the second is obtuse, while the third is cambered.

基于三维、不可压、定常N-S方程和k-ε双方程湍流模型，采用有限体积法对3种不同设计方案在运行时速为160 km/h的集装箱专用平车在有、无横风情况下的气动性能进行分析与比较，其中：方案一的平车无端墙，侧梁侧面平直；方案二和三的平车均加有端墙，且侧梁加高、侧面形状分别为钝形和弧形。

Based on 3D steady and incompressible NS equation and kε turbulent model, the aerodynamic performance of three different design options of 160 km/h container flat wagon was analyzed and compared using finite volume method. The three design options are as follows: the first design option has no headwall and the side surface of side beam is flat; the second and the third both have headwall and high side beam, and the side surface of side beam of the second is obtuse, while the third is cambered.

摘 要：基于三维、不可压、定常 N S 方程和 k ε双方程湍流模型，采用有限体积法对3种不同设计方案在运行时速为160 km/h的集装箱专用平车在有、无横风情况下的气动性能进行分析与比较，其中：方案一的平车无端墙，侧梁侧面平直；方案二和三的平车均加有端墙，且侧梁加高、侧面形状分别为钝形和弧形。

Methods Thirty eight patients with loosening of hip prosthesis were divided into three group: total hip arthoplasty, double hip arthuplasty and integrated arthoplasty. The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph, and the content of TNF in samples of interface membranes was determined.

方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分为3组：全髋置换组、双动头组和一体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计数的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

［Objective］To study the relativity during polyethelene debris cytokine and loosening of hip prosthesis.［Methods］Thirty eight patients with loosening of hip prosthesis were divided into three group:total hip arthoplasty,double hip arthoplasty and integrated arthoplasty.The inflammative reaction was analyzed with light microscopic observation arrording to the half-ration standard of Joseph,and the content of TNF in samples of interface membranes was determined.

［目的］研究聚乙烯微粒、细胞因子与人工髋关节松动的相关性［方法］根据假体的类型及假体中是否含有聚乙烯将38例人工髋关节翻修病例分 3组：全髋置换组、双动头组和体式单动头组，对各组术中松动假体周围界膜组织进行光镜下炎性细胞计的半定量分析和肿瘤坏死因子的测定，分析人工髋关节无菌性松动与聚乙烯微粒、细胞因子之间的相关关系。

METHODS: The Medline, Science Direct On Site, and Springer database were searched in detail on the data of the mycological cure rates of the two antifungal agents for treatment of dermaphyte onychomycosis occourring in patients aged from 18 a to 60 a with the published double blind randomized clinical trials and then pooled. The odds ratio and its 95 ﹪ confidence interval were calculated.

检索Medline等数据库，查找所有比较特比萘芬与伊曲康唑治疗18～60 a无系统疾病所患甲癣的双盲随机对照试验的文献，记录这2种药物治疗甲癣的真菌学治愈率并对其进行汇总分析，得出合并后真菌学治愈率的优势比及其95﹪的可信区间。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失