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In vitro, we cultured human and rabbit retinal Müller cells by way of mixed enzyme digestion and tissue adherence, using human retina of an abortus of 6 months and healthy rabbit.

取孕6个月引产胎儿,死后2h内无菌条件下摘取眼球,剥取视网膜组织,混合酶消化法培养人视网膜Müller细胞;取健康新西兰大白兔,死后以最快的速度无菌条件下摘取眼球,组织块贴壁法培养兔视网膜Müller细胞;利用含胎牛血清的高糖DMEM作为培养液,不添加生长因子。

Isolates isolated from maize and 3 isolates from rice, wheat, adzuki bean in ten provinces were identified as Curvularia lunata. Based on the culture characters, such as color of colony, aerial myeelia, the number of conidia, there were some difference among 43 isolates. Meanwhile, five types of conidium were compared in culture characters and virulence. In each single conidia culture there were different morphic conidia also and no difference in virulence was found among five single-conidium cultures.

收集并分离我国10个省的玉米叶斑病病原物40个以及水稻、小麦、红小豆上的C.lunata分离物3个;对分离物进行培养性状、产孢能力及形态特征的研究结果表明:分离物在菌落颜色、气生菌丝的繁茂程度、产孢量的多少等方面存在明显的差异;同一分离物内分生孢子在形态方面有差异,不同形态单孢继代培养后仍出现多种形态分生孢子,但各单孢培养系在致病力上无差异。

A large nocturnal burrowing rodent of the genus Cuniculus, found in South and Central America and similar to the agouti, especially the spotted species C.

无尾刺豚鼠一种夜间打洞的天竺鼠属大啮齿目动物,产于中美洲和南美洲,和刺豚鼠类似,尤指有斑点的种类加拿大无尾刺豚鼠,依靠植物和水果为生,因其肉可食而被捕猎

A large nocturnal burrowing rodent of the genus Cuniculus,found in South and Central America and similar to the agouti,especially the spotted species C.paca that lives on plants and fruit and is hunted for its edible flesh.

无尾刺豚鼠一种夜间打洞的天竺鼠属大啮齿目动物,产于中美洲和南美洲,和刺豚鼠类似,尤指有斑点的种类加拿大无尾刺豚鼠,依靠植物和水果为生,因其肉可食而被捕猎

In this section, we focused on the investigation of the condensation, Michael reaction of different aromatic aldehyde with different aryl substituted ketones under solvent-free and PTC conditions, obtained polysubstitutedcyclohexanol derivatives in mordent yield when using TBAB as the catalyst.

通过实验发现,TBAB对该反应具有良好的促进作用,使在液相中需十几个小时甚至几十个小时才能完成的反应,在无溶剂条件下几分钟就可完成,并且使液相中两步完成的反应,而在无溶剂条件下一步就可完成,且产率优良。

All experiments were carried out under the dry and oxygen-free argon atmosphere by using standard Schlenk techniques.

在无水无氧和纯的氩气保护下,使用Schlenk技术,用2-(C1CH2) C5H4N·HC1与2当量的C9H7Li在THF中冰水浴反应,以73%的产率得到3-(2-C5H4NCH2)C9H7(1)。

The results show that the resulting product can be separated by the mixture of petroleum ether, aether and acetic acid (10:10:1, V/V). Among them, the oleic acid component can be eluted by the mixed solvent of ethanol and carbon tetrachloride (35:15, V/V), and then productivity of sodium oleoyl sarcosine can be obtained.

结果表明:用石油醚:无水乙醚:冰乙酸=10:10:1分离产物中的组分,再用无水乙醇:四氯化碳=35:15混合溶剂洗脱制备油酸组分,进而计算油酰肌氨酸钠的产率是可行的。

For Southwestern Margin of Aqikekule Lake ophiolite, their field geological, petrological, minerological, petrochemical and geochemical characteristics show that:① it outcropped as tectonic slices along the near west-to-east strike Kunzhong fault and is composed of metamorphic perodotties, cumulates and volcanic rocks, in which, chromites are distributed in the upper part of metamorphic peridotites as pods, or in the lower part of cumulates as near-strata;② metamorphic peridotites include serpentinites, chromite-bearing serpentinites, chlorite-epidote schists and chromitites, of which, chromitites have nodular and orbicular structure, and cumulates include pyroxenits, serpentinites, chromite-bearing serpentinites, chromites and metamorphically mafic rocks and only basalts are seen in volcanic rocks;③ Cr# of chromites suggest that they formed in the SSZ and Al〓O〓 and TiO〓 of metamorphic peridotites also suggest SSZ environment;④ metamorphic peridotites have V type and enriched LREE patterns, cumulates have from strongly depleted LREE, flat REE to enriched LREE patterns with universally striking positive Eu anomalies and basalts show flat REE or slight enriched LREE patterns with no Eu anomalies;⑤ trace element and Sm-Nd isotope characteristics of metamorphic peridotites imply their strikingly heterogeneous mantle character ε〓(t+4.39~+26.20 and later Nb, Ta fertilization;⑥ trace element characteristics of basalts and their tectonic diagrams show they probably formed in the rifted island arc or back-arc basin environment.

对阿其克库勒湖西南缘蛇绿岩,野外地质、岩石学、矿物学、岩石化学以及地球化学等特征表明:①该蛇绿岩以近东西向、沿昆中断裂呈构造岩片出露,由变质橄榄岩、堆晶岩和火山岩三个单元组成,其中见铬铁岩呈豆荚状产于变质橄榄岩的上部,或呈似层状产于堆晶岩的下部;②变质橄榄岩包括蛇纹岩、含铬铁矿蛇纹岩、绿帘绿泥片岩和铬铁岩等类型,其中,豆荚状铬铁矿具有结核状构造和球状构造,堆晶岩包括辉石岩、蛇纹岩、含铬铁矿蛇纹岩、铬铁岩和变质基性岩等类型,火山岩仅见玄武岩;③铬铁矿的Cr#表明其形成在俯冲带,变质橄榄岩的Al〓O〓和TiO〓成分也指示了SSZ环境;④变质橄榄岩的稀土配分型式见V型和LREE富集型两种型式,堆晶岩表现出从LREE强烈亏损、REE平坦分布到LREE富集的配分特征,并且普遍具有醒目的正Eu异常,玄武岩的稀土配分型式表现为平坦型或LREE略富集,无Eu异常;⑤变质橄榄岩的微量元素及Sm-Nd同位素特征指示了其地幔的显著不均一性ε〓(t为+4.39~+26.20以及Nb、Ta在后期的富集;⑥玄武岩的微量元素特征及构造图解表明其可能形成在裂谷岛弧或不成熟的弧后盆地环境。

Additionally, the growth of the pathogen was obviously affected by cultural media. The morphological characters such as colony color and diameter, and production of fruit body of the fungus growing on PDA, 10% V8, apple leaves dextrose agar and apple leaves extraction dextrose agar media were different. The fungus formed colonies of about 8mm in diameter and did not produce fruit bodies and aerial hyphae in 1 month incubation on PDA. However it formed the similar size colonies as on PDA and produced fruit bodies and aerial hyphae on 10% V8 and LDA media in the same incubation period. Very small colonies (2mm in diameter) and fruit bodies were found on LEDA media in the same incubation condition. These results indicated that successful isolation of M. coronaria from apple leaves depended on suitable isolation method and cultural media as well as fresh samples.

不同培养基上菌落形态、大小和产孢情况差异也很大,培养1个月(25℃)后PDA上菌落黑褐色隆起,表面蚯蚓粪状,无气生菌丝,无子实体和基内菌丝;10% V8培养基上菌落中央隆起,黑褐色,表面生少量气生菌丝,边缘放射状,基内菌丝深褐色,有子实体;苹果叶片葡萄糖琼脂培养基上菌落平坦,黄褐色,表面生茂密的金黄色气生菌丝,基内菌丝深褐色,有子实体;苹果叶片煎汁葡萄糖琼脂培养基上菌落有明显的不规则隆起,黄褐色至黑褐色,表面生少许气生菌丝,菌落生长缓慢,无基内菌丝,分生孢子盘菌落表面生,菌落直径仅2mm左右,而在其他培养基上的菌落直径可达6-8mm,说明培养基质、分离方法均对苹果盘二孢的分离培养和生长发育有明显的影响。

Electricity production in the mediator-less two-chambered microbial fuel cell was researched. Based on the result in the different operation phase in the MFC, the microbial diversity was analysed.

中文摘要本文对无介体双室微生物燃料电池的产电性能进行了初步研究,并根据不同运行阶段产电性能的优劣,对其中微生物的差异性进行了比较分析。

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