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Bis (P—bromobenzylamino) ethane and 1,2—bis (m—Iodobenzylamino) ethane may be prepared by condensation of the corresponding halo benzaldehyde with ethylenediamine and followed by catalytic hydrogenation of the Schiff's bases over Pto_2 in glacial acetic acid solutions: Both Ⅱa and Ⅱb may react in neutral mediam with aldehydes to form well—defined crystalline tetrahydro—imidazole, which may be served as derivatives of identification for aldehydes: Under the same condition, however,Ⅱb may ...

用1,2-二乙烷与二十余种醛类作用,得到有固定熔点的晶体衍生物,它们的熔点分布在85—210℃之间,产率为40—96%,这试剂与甲基酮类在相同条件下无反应,因此,它可作为鉴定醛类的专属试剂。 2。上述衍生物用稀盐酸在室溫处理,能发生水解,可以定量地收回原来的醛,因此这试剂又可作为分离醛类的试剂。 3。上述衍生物可以用非水滴定法半微量地测定其分子量可作为鉴定醛类的另一项参考数据。 4.1,2-二乙烷与醛类能生成晶体衍生物,但这试剂也与甲基酮在同样条件下发生反应,所以不适合作为鉴定醛类的专属试剂。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Biological characteristics of the pathogen and the resistance of 13 cultivars of tea plant to tea brown blight were studied by means of natural infection in the field and artificial inoculation in vitro. Resistant cultivars and susceptible cultivars of tea plant were divided. And the resistance mechanism was researched tentatively. The findings are as follows: 1 Some biological characteristics of the pathogenic fungus The experiment result showed: the optimum temperature for the growth of fungus mycelium was from 24 to 27℃, the optimum temperature for sporulation ranged 22 to 30℃. Treated at 54℃ in 10 minutes, the spores didn't germinate. The optimum pH for the growth of the mycelium was 5.1~5.9, the optimum pH for sporulation ranged 5.0~6.0. Light had no much effect on the growth of fungus mycelium, but had much effect on the reproduction of the spore.

首先,研究了茶云纹叶枯病病菌的生物学特性;其次,通过田间抗性调查和室内人工接种鉴定,对 13 个茶树品种进行了抗病性鉴定;第三,在前人研究的基础上,对茶树抗病机理作了更深入的研究,结果发现: 1 病原菌的生物学特性病原菌的分生孢子萌发的最适温度范围为 24~27℃,菌丝生长的最适温度范围为 22~30℃;致死温度为 55℃;菌丝生长的最适 pH 范围为 5.1~5.9,孢子萌发的最适 pH 范围是 5.0~6.0;光照对病菌生长速度及分生孢子萌发影响不大,但对其产孢的影响很大,特别是有光照与无光照处理之间差异显著,光照有利于分生孢子的形成。

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