无上的

Screws, bolts, or nuts without washers must be loaded against a test plate with a minimum diameter clearance hole equal to the nominal thread size plus 0.50mm for fasteners M6 (1/4 inch) and smaller, and plus 1.0mm for larger diameters.

无垫圈的螺钉、螺栓、或螺母应被装夹到具有较小孔径的通孔的试验圆板上进行试验，对于≤M6 (1/4 inch)的紧固件，试验圆板的孔径为公称螺纹直径加上0.50mm，对于＞M6 (1/4 inch)的紧固件，试验圆板的孔径为公称螺纹直径加上1.0mm 。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

In chapter 2, this text mainly is an analysis to be the business enterprise to implement the society to carry the basic problem of the public-spirited foundation of body- business enterprise public-spiritedly, analyzing the business enterprise definition, type, social function of th 本文来自:无忧论文网 51lunwen.com

在第二章中，本文主要是分析作为企业履行社会公益的载体——企业公益基金会的基本问题，在结合了公益基金会理论的基础上分析了企业公益基金会的定义、类型、社会功能，其与一般慈善组织的区别联系，并进一步分析指出了企业公益基金会作为企业社会公益载体的重要组织形式的原因

The gloved hand should not come in contact with the skin before the incision is made. For the extremities, a section of sterile stockinette is drawn proximally over the operative field. Then the stockinette is grasped proximally and distally and cut with scissors to uncover the area of the proposed incision. Its cut edges are pulled apart, and the area is covered by a transparent adhesive-coated material.

带手套的手在切皮之前不许与皮肤接触，肢体手术可应用弹力织物向近端套于手术区表面，然后抓住弹力织物的近端及远端，于木野处用剪刀剪开，将剪开的边缘拉开，在暴露的术野的皮肤上贴无菌的皮肤贴膜。

The gloed hand should not come in contact with the skin before the incision is made. For the extremities, a section of sterile stockinette is drawn proximally oer the operatie field. Then the stockinette is grasped proximally and distally and cut with scissors to uncoer the area of the proposed incision. Its cut edges are pulled apart, and the area is coered by a transparent adhesie-coated material.

带手套的手在切皮之前不许与皮肤接触，肢体手术可应用弹力织物向近端套于手术区表面，然后抓住弹力织物的近端及远端，于木野处用剪刀剪开，将剪开的边缘拉开，在暴露的术野的皮肤上贴无菌的皮肤贴膜。

When two communicants and an adversary obtain correlated information through independent binary symmetric channels from a random source, and the adversary's channel is noisier than those of communicants, an authentication scheme which uses the correlated information between the two communicants is proposed based on the coding theory to make possible information theoretic secret key agreement against active attacks.

在无条件安全密钥协商中，假定通信双方通过相互独立的无记忆二元对称信道来接收二元对称信源所传送的信息作为初始信息，在这种条件下，本文提出了一种利用他们之间的初始相关信息对公共信道上的消息进行认证的具体方案，从而使得无条件安全密钥协商具有抗主动攻击的能力。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

Keeping pets is not prohibited, but bringing a pet onto a bus, especially a crowded bus, is at least inappropriate and illmannered, if not uncultured and illegal.

第三句话说养宠物并不被禁止，但是带宠物上公交车，即使不是无修养的和非法的，也是不合适和不礼貌的。该句中的难点是if not的用法，这里该结构中共用了四个词，分两个明显不同的层级，在同一层级中，后一个又比前一个程度深一些。

RP originates historically in the southeast of England and is spoken by the upper-middle and upper classes throughout England. It is widely used in the private sector of the education system and spoken by most newsreaders of the BBC network.

它以受过教育的人说的南部英国英语的语音为基础，无明显的地区特征，是社会上受过良好教育的人通常采用的口音，由于在学校教育、外语教学及广播中的广泛采用，其影响较大，并且常被推荐给外国人学习英语发音。

The shuttle enabled its astronauts to see, every 90 minutes, how the sun ignited a giant rainbow over the morning Earth. It allowed them to observe the still, fixed colours of the stars; to watch from the outside as lightning forked through a weather front; to measure the spiders' webs of cities against the immense blue of ocean and the matt black of far space.

在飞船上，宇航员每隔90分钟就可以看见太阳如何在晨曦的地球上放点燃绚丽的巨型彩虹，观察星球永恒不变的颜色，从外界欣赏闪电如何叉入大气层，在浩瀚的蓝色海洋和暗淡无光的外空测量城市形成的蛛网。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失