无上

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The increase in WHC of resistant starch having guar gum over resistant starches with guar gum and different chemical agents could be ascribed to non interference in the chemical configuration and composition especially in greater number of hydrophilic sites on the gum that allows the occlusion of water within the structure.

单独含有瓜尔豆胶的抗性淀粉其WHC比含有瓜尔豆胶和其他化学试剂的抗性淀粉高是因为在胶上一些亲水基团上无化学构象，这导致了水在结构中的封闭。

Narration characteristics include love narration, experience narration and simple images.The novel indicates Zhang Jie new accomplishment, which consolidates her position of female literature history by her sharp-cut view, steady standpoint, profound thought and spiritual edification.

无字》的出现，代表着张洁对女性命运的关注与认识走向了一个成熟的审美境界，她鲜明的观点，坚定的立场，深刻的思考，以及思想上的启迪性，都代表了新世纪以来女性文学的又一大成就，也巩固了张洁在女性文学史上的重要地位。

In light of the characteristics of the network media, and the observation of forms of vision culture which disseminates on the network platform, including Network Visual Communication Design, Network News Photos, Gibberish Pictures, Flash Shake Culture, Network Imaging Video and Network Games.

在已有的关于视觉文化传播文献的基础上，结合网络媒体的特性，观察视觉文化在网络平台上传播的表现形式，包括网页视觉传达设计、网络新闻图片、无厘头图片、Flash闪客文化、网络视频影像和网络游戏。

The main target stratum has the following characteristics: dominated by barrier-free clastic tidal facies, tidal facies are divided into three subfacies: supratidal zone, intertidal zone and subtidal zone. The tidal facies in Tazhong Area are mainly dominated by intertidal sand flat, sand-mud-mixed flat, intertidal mud flat and supratidal mud flat subfacies.

研究表明研究层段主要发育无障壁碎屑潮坪沉积相类型，潮坪相进一步区分出潮上带、潮间带和潮下带三个亚相，塔中地区潮坪沉积主要发育潮下带潮汐水道、潮间带砂坪、砂泥混合坪和潮间带泥坪及潮上带泥坪等微相。

Of the second scenario, the end of the discharge pin board focusing ring rust to deal with the general use of anhydrous alcohol to clean the discharge end of seat ring and plug and socket of the electrode, such as severe corrosion ring discharge, Tailstock replaceable parts.

对第二种情况，尾板上聚焦引脚放电环锈蚀的处理，一般使用无水酒精清洗放电环及尾座上各电极插头和插座，如放电环锈蚀严重时，可更换尾座部件。

The amount of teeth loss, wearing removable partial denture or not and the classification of Eichner index show directly in the discomfort performing chewing function, as respect of the subjective feelings of the volunteer.

而缺牙数、有无配戴活动假牙以及Eichner index，对於受试者主观的感受，直接的反映在口腔咀嚼功能不适上，故在量表结果上反映出与生理功能有关的结果。

The results showed that the performance of the drill pipe joints was in line with standard and the main reason was that the making up torque was inadequate.Drill pipe joints in underground unrestrained tightening-up caused the drill pipe joints to bear the excessive load more than their anti-torque yield strength.

结果表明：钻杆接头的各项性能指标均符合标准规定，失效的主要原因是上扣扭矩不足，钻杆接头在井下无节制旋紧，致使钻杆在井下的自上扣扭矩超过了钻杆接头的实际抗扭屈服强度。

On tubeless tire s, do not use tire irons to force the beads away from the rim flanges; this could damage the rim seals on the beads and cause an air leak.

1维修无内胎轮胎时，不能使用撬棒强行把轮缘从轮辋上拆卸下来。这样容易损坏轮缘上的轮辋止口，并造成空气泄漏。

I would thou couldst; For who would bear the whips and scorns of time, The oppressor's wrong, the proud man's contumely, The law's delay, and the quietus which his pangs might take, In the dead waste and middle of the night, when churchyards yawn In customary suits of solemn black, But that the undiscovered country from whose bourne no traveler returns, Breathes forth contagion on the world, And thus the native hue of resolution, like the poor cat i' the adage, Is sicklied o'er with care, And all the clouds that lowered o'er our housetops, With this regard their currents turn awry, And lose the name of action.

但是那世人有去无还的冥界，正向人间喷出毒气阵阵，因此那刚毅的本色，象古语所说的那只可怜的小猫，就被烦恼蒙上了一层病容，一切压在我们屋顶上的阴云，因此改变了漂浮的方向，失去了行动的力量。那正是功德无量。且慢，美丽的峨菲丽雅：别张开你那又大又笨的大理石嘴巴，赶快到女修道院里去吧——快去①。①《文库》本注：&公爵&自以为在背诵哈姆雷特这段著名独白，其实背得颠倒错乱，还胡乱插进了莎剧《麦克白》和《理查三世》中的台词。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。