方法研究

Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel Homo-and heteromeric ASIC complexes have been verified by classical methods such as co-immunoprecipitation,or functional assays applying electrophysiology.However,the exact subunit assembly and stoichiometry of heteromeric ASIC channels in living cells are largely unknown.

用荧光共振能量转移方法研究酸敏感离子通道的装配用经典的方法如免疫共沉淀或电生理分析，认为大多数亚单位可以形成同聚体或异聚体复合物，然而关于活细胞中ASICs亚单位装配以及ASICs异聚体中各亚单位分子比的情况并不清楚。

A Chinese-English word alignment algorithm based on bilingual dictionary is introduced. It is inspired by Ker's method.

在Ker的基于类的词语对齐方法研究和实践的基础上，提出了一个利用大规模双语词典进行汉英词语对齐的方法。

In Chapter 4, Short-time dynamic behavior of two-dimensional fully frustrated XY model near the Ising-like phase-transition temperature is studied with resistively shunted junction dynamics. The transition temperature and the dynamic and static critical exponents are estimated under both a periodic boundary condition and a fluctuating twist boundary condition using the short-time dynamic scaling analysis.

第四章应用电阻分流结动力学方法研究了完全阻挫XY模型在Ising型相变温度附近的短时动力学行为，运用短时动力学标度分析方法，我们得出了该模型在周期性边界条件下和涨落扭转边界条件下Ising型相变的临界温度和各种临界指数。

With routine method, from having elected contents higher one RNA bacterial strain among several saccharomycetes, beer yeast, and the method with Shan factor experiment have studied an experiment being bacterial strain's turn to produce more proper RNA culture medium made up of , optimum the pH value fermenting and the best fermentation time.

本实验用常规方法从数株酵母菌中选出了一株RNA含量较高的菌株——啤酒酵母，并用单因素实验的方法研究了该菌株产RNA较适宜的培养基组成、最佳发酵pH值和最佳发酵时间。

Simultaneously we prepared benzimidazolines with two methods by the reducton of benzimidazolium salts with NaBH_4 and Grignard addition with benzimidazolium salts. Thus we provided a new biomimic synthetic method for 9 kind of tetrahydro-β-carboline starting from carboxylic aicd and halid. A convenient synthetic method for preparing tetrahydro-β-carboline was provided.

本文以1，3-二甲基苯并咪唑碘盐和2-取代的1，3-二甲基苯并咪唑碘盐为原料，采用了NaBH_4还原和Grignard试剂加成两种方法，制备了9种1，3-二甲基苯并咪唑烷和2-取代的1，3-二甲基苯并咪唑烷作为四氢叶酸辅酶一碳单元转移新模型，在酸性条件下，以色胺作为接受一碳转移的亲核试剂，完成9种四氢-β-咔啉类化合物的新合成方法研究。

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

The purpose of this paper is the analysis、computation and dynamic equivalence of power system frequency dynamics as well as the designation and implementation of underfrequency load shedding scheme. Some important conclusions have been reached based on detail theoretical analyses and a large amount of calculation results.

本文采用理论分析和详细时域仿真相结合的方法研究了复杂扩展式多机电力系统中功率—频率动态过程的主要特点，讨论了频率动态过程的计算方法，解决了频率过程计算中的动态等值问题，同时对电力系统低频减载方案的设计和实施问题进行了深入的探讨。

Simultaneously we prepared benzimidazolines with two methods by the of reduction benzimidazolium salts with NaBH_4 and Grignard addition with benzimidazolium salts. Thus we provided a new biomimic synthetic method for 13 kind of tetrahydroisoquinoline starting from carboxylic aicd and halid, gave a new idea of the synthesis for new drugs.It can make many kind ofβ-carboline alkaloids which have bioactivity to antibacterium, antivirus and antitumor to start from 1,2,3,4-tetrahydro -β-carboline.

本文以1，3-二甲基苯并咪唑碘盐和2-取代的1，3-二甲基苯并咪唑碘盐为原料，采用了NaBH_4还原和Grignard试剂加成两种方法，制备了13种1，3-二甲基苯并咪唑烷和2-取代的1，3-二甲基苯并咪唑烷作为四氢叶酸辅酶一碳单元转移新模型，在酸性条件下，以2-(3，4-二甲氧基苯基)乙胺作为接收一碳转移的亲核试剂，完成了13种重要的四氢异喹啉类化合物的新合成方法研究。

In the CGE model, the paper introduces the CGE model and its application in the tax field, the framework for solution of CGE model, the calibration of CGE model, using GAMS to solve the CGE model with MCP and NLP arithmetic, also the paper use the Three sector data of the National Account to do some application.

在CGE模型方法研究中，介绍了CGE模型及其在税收收入预测中的应用和求解CGE模型的理论框架、应用GAMS求解CGE的简单回顾、CGE模型中的SAM、CGE模型的估计与校准、使用GAMS求解CGE模型、应用GAMS求解CGE模型的NLP方法、应用GAMS求解CGE模型的MCP算法等内容，同样也结合国民经济三部门的税收经济数据进行了简要的应用。

The third part touches upon the enlightenments gained from the study on the relationship between paradox and mathematical logic on the relationship between paradox and mathematical logic, which are the following: so long as we combine the way of formalization with the philosophizing analysis, look at things dialectically, and deal with things systematically, not only can the problem of paradox be solved relatively, but also a series of important discovery can be found in the process of resolution to it.

第三部分论述在探析悖论与数理逻辑的关系中所得到的意义和启示：只要我们把形式化的方法和哲学性的分析结合起来，用辩证的观点看问题，用系统的方法研究问题，悖论不但可以得到相对的解决，而且在解决悖论的过程中会引出一系列的重大发现。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。