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To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum(1.562 5-200 ng/ml). Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment (350 bp)had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性,采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度,取高纯度的DNA样品用灭菌水稀释,分别取不同量的DNA进行PCR扩增,确定PCR方法的敏感性;利用该方法对32份奶牛流产的胎牛脑组织样品进行检测,同时,对其中23份流产的母牛血样进行ELISA血清学检测,以评价犬新孢子虫PCR方法的检测效果。

This thesis try to use the risk decision, Bayes formula and game theory to analyze the utility of the traffic information, and put forward the corresponding static and dynamic model.

本论文在总结国内外交通信息效用研究理论与方法的基础上,系统地对不考虑交通信息有效性反馈和考虑交通信息有效性反馈两种条件下交通信息的作用机理进行研究,尝试采用风险决策、贝叶斯推理、博弈论等理论和方法建立了相应的模型,并对影响交通信息效用的交通信息质量、交通系统稳定性、出行者的出行经验等因素进行了分析,提出了增加交通信息有效性的方法。

The results show that the infinite method is valid in solving problems in infinite domain and thus high precision can be obtained by eliminating the border effect in finite element analysis.

采用具有精确解的无限厚壁圆筒作有限单元法分析和无限单元法分析,通过对这两种分析方法的分析结果的比较得知:无限单元法是解决无限域问题的一种有效的方法,采用这种分析方法进行隧道及地下工程结构分析,可以消除有限单元法所带来的边界效应,从而使计算精度大为提高。

EXPERIMENTAL STUDY ON THE INFLUENCE OF PROCESSING ON THE MECHANICAL PROPERTIES OF ENGINEERING CERAMICS Fan Ning Ai Xing Deng Jianxing Wang Yixing (Shandong University of Technology) Abrstract The mechanical properties of engineering ceramic are impacted by itself and its surface status.

加工方法对工程陶瓷力的影响摘要工程陶瓷的力学性能受材料本体和表面状况的共同影响。加工方法对材料的表面状况有较大的影响,因此,陶瓷材料的力学性能也受加工方法的影响深圳陶瓷加工工业陶瓷加工

This paper reviews the major methodological issues in cross-cultural research,covering topics in research design,measurement,and data analysis.From a design perspective,the paper begins with the use of consilience methods to strengthen causal inferences in cross-cultural research.Th...

作者首先从研究设计的角度分析如何通过汇聚方法加强检验跨文化研究的因果关系的能力,然后分析跨文化研究中测量对等性(measurement invariance/equivalence)问题以及相关的统计方法,最后简要介绍了多层线性模型在跨文化研究中的应用、并讨论跨文化研究方法的发展趋势。

In order to verify the method, the comparison among the Tabary slope method, the Bayes function method, the Yamada continuity method and the background error covariance function method is presented. The results show that the background error covariance function method is the best one.

为检验此法效果,利用1999年5月3日到4日奥克拉荷马强龙卷的雷达资料,给出风廓线仪的实测结果对比分析,Tabary的切线拟合方法和条件概率方法,Yamada的连续性检测方法的对比试验及退模糊后的径向速度图。

By applying contour integral method and high-order displacement functionsof MM,we obtain the accurate stress intensity factors of mixed-mode crack.Then,the open crack propagation of the mixed mode I and II is simulated by means oftheories of linear elastic fracture mechanics.This provides a simple and effectivenumerical way for crack evolvement studying.

接着,文中采用围线积分法和流形方法的高阶位移函数来计算得到比较准确的混合型裂纹尖端的应力强度因子,然后基于线弹性断裂力学的裂纹扩展准则,用流形方法实现了平面I-II混合型张裂纹扩展的数值模拟,为数值方法研究裂纹的演化行为提供了一条简单而有效的途径。

After summarizing the existing research results, this article proposes new methods on moving force identification based on radial basis function approximation method and cubic spline function approximation method, and discusses the influence which some parameters to the precision of identification through calculating an example.

文章首先在第一章中介绍了已有的移动荷载识别方法及国内外的研究状况,在此基础上提出了研究目标和意义,即提出新的移动荷载识别方法,并通过数字模拟来讨论新方法的精度,以便评估桥梁的动力特性,为桥梁的设计和汽车性能的改进提供依据。

On the basis of comparing the black-spots identification methods at home and abroad, by analyzing the accident statistical data of national road 312, the paper puts forward the accident cumulative frequency method that can find the black-spots in the road, and make some improvement considering various comprehensive traffic safety factors.

在比较国内外现有事故多发位置鉴别方法的基拙上,通过对我国312国道某路段事故交通状况和事故统计资料的分析,提出了适合此路段的事故多发位置的鉴别方法—累计频率法,并在实际应用中从综合考虑各种交通安全评价因素的角度出发,基于当量和数理统计的理论对此方法进行了改进。

A simplified method was proposed to deal with the boundary condition at the turbine unit for transient calculation. This method is based on the statistical analysis of turbine hill chart and the Eular equation for water turbines. According to the Eular equation, the basic geometry parameters of the turbine, such as guide vane opening a, blade outlet angle β2, the relative blade width of outletb2, the relative height of wicket gateb0 and the relative turbine exit radiusr2 are obtained through curvilinear regression of the water turbine hill charts.

为解决目前水轮机过渡过程计算中确定水轮机边界条件时通常使用的外特性法和内特性法的不足之处,结合这2种方法的优点,提出一种在水轮机过渡过程计算中处理水轮机边界条件的方法,即根据综合特性曲线的数据,利用统计分析方法和水轮机基本理论公式,计算水轮机基于内特性解析方程的基本参教,如出流角α、转轮出口安放角β、转轮出口相对宽度b2、导叶相对高度b0、转轮出口相对半径r2,在特性曲线上的规律。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。