方法的

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

To establish new methods for highly sensitive time-resolved fluorescence bioassay, several new lanthanide fluorescence complexes were synthesized and used for the preparations of nanometer-sized lanthanide fluorescence biolabels. Novel lanthanide fluorescence nano-biolabels are uniform (25-55 nm in diameters),strongly fluorescent with long fluorescence lifetime, highly photostable and biocompatible.

中文摘要：本项目以时间分辨荧光生物分析新方法的建立与应用为研究目的，在合成出几种稀土荧光配合物的基础上，建立了几种新型强荧光性纳米稀土荧光生物标记材料的可控制备方法，生物标记方法及其在时间分辨荧光免疫分析中的应用方法。

Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

方法嗜肺军团菌与多噬棘阿米巴共培养，取不同时点的共培养物制作涂片，采用革兰氏染色、吉曼尼兹染色、姬姆萨染色、免疫荧光染色等多种方法，用光学显微镜及荧光显微镜观察鉴别阿米巴滋养体与其胞内嗜肺军团菌，并比较这些染色方法的效果。

A new method that formaldehyde in air is collected in canister, preconcentrated through 3-stage traps and analyzed by GC/MS is introduced in this paper. Characters such as detection limit, veracity, stability and region of linear response are investigated.

本文研究了采用SUMMA罐采样，三级冷阱预浓缩，GC/MS分析室内空气中甲醛的方法，并考察了该方法的检出下限、准确度、精密度、线性响应范围等方法特性。

For three different reaction paths leading to ortho, meta, para-chloropyridine, two transition states have been located by PM3 on each reaction path, while only one transition state by ab initio, however both methods give the same results: the energy of transition state and activation barrier on producing 2-chloropyridine are the lowest among three reaction paths, so 2-chloropyridine is obtained priorly, which agrees with the experimental results.

生成邻、间、对氯代吡啶三条不同反应途径PM3方法优化得到两个过渡态，而从头算方法中只优化得到一个过渡态。但两种方法的研究结果均表明：三条反应途径中生成2-氯吡啶的过渡态能量和活化能最低，因此反应优先生成2-氯吡啶，与实验结果一致。

The wavelet time-frequency properties of the uncracked rotor and the cracked rotor were discussed by the continuous wavelet time-frequency analysis algorithm. A new algorithm using the wavelet time-frequency analysis algorithm to detect the crack was proposed. The influence of the sampling frequency on the wavelet time-frequency analysis algorithm was analyzed through the numerical simulation research; the valid sampling frequency was presented.

利用连续小波时频分析方法，讨论了裂纹转子与无裂纹转子的小波时频特性，提出了利用小波时频分析方法识别裂纹的新方法；数值仿真研究了采样频率对小波时频分析方法的准确性和有效性的影响，并给出了建议性的采样频率。

Methods The method of methylation and microwave synthesis method of microwave radiation Veratryl aldehydes, synthesis of these two methods compare the yield of the product and the fundamental nature, analyzing the advantages and disadvantages of both methods.

采用甲基化的方法和微波微波辐射的方法合成藜芦醛，比较这两种方法合成产物的产率及基本性质，分析两种方法的优劣。

Rational unified process agglomerating the wisdom of all kinds of Software engineering methods is a focus.

Rational统一过程方法凝聚了各种软件工程方法的智慧，成为对软件工程方法研究和运用的焦点。

Textile colorfastness to crocking is very important, and there are differences among test methods in different countries. The test results are influenced by different test methods. In order to avoid the "trade rubs" among different countries, it is necessary to know the correlation among test results of different test methods. In this paper, Chinese GB(GB/T 3920-51997), AATCC(AATCC TM 82004) and JIS(JIS L 0849-1996) test methods of colorfastness to crocking were introduced.

纺织品耐摩擦色牢度是重要的检测指标，各国的标准和测试方法有一些差异，而不同的测试方法对测试结果有一定的影响，为了解不同测试方法测试结果的相关性，以避免贸易摩擦，本文介绍了耐摩擦色牢度的我国国标(GB/T 3920 5 1997)、美国纺织化学家及染色家协会标准(AATCC TM8 2004)、日本标准工业协会标准(JIS L 0849 1996)等三个测试方法标准，通过对相同试样的三种测试方法的测试，探讨了评定值之间的相互关系。

Introduces and analyzes various demodulating methods of fiber grating, concludes characteristics of every demodulating methods, and does comparison of each demodulating methods.

介绍和分析了光纤光栅的各种解调方法，归纳了各种解调方法的特点，对各种解调方法做了比较。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。