方法

Method］ARBX was used to treat 24 cases of chronic hematogenous osteomyelitis as one-stage grafting after debridement since September of 2001, 17 cases of which were followed up for an average period of 34 months (range, 12 to 79 months).

方法］ 在获得总后卫生部军内临床试用研究许可的基础上，自2001年9月起将ARBX用于临床治疗慢性血源性骨髓炎24例，对其中17例获得12个月以上系统随访的患者进行疗效分析。

Such is the basic principle underlying the Hesychast 'method'.

这就是静修者提供的&方法&中所有的基本原理。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Abstractive】Objective:To observe the efficacy of photodynamic therapy combined with intravitreaous injection with Avastin in one patient of capillary angioma of the opic papillary.Methods:One patient,the visual acuity was 20/200,were diagonosed as Lindau-von Hipple disease with capillary angioma of the opic papillary by B scan and Cranial Computed Tomography.

目的：对光动力疗法联合玻璃体腔Avastin注射治疗对1例von Hipple－Lindau病中视乳头毛细血管瘤的疗效观察；方法：经眼底荧光造影以及B超和颅脑CT检查确诊为von Hipple－Lindau病伴视乳头毛细血管瘤，最佳矫正视力为0.1，FFA示视乳头荧光渗漏面积为11平方毫米。

Objective To evaluate the role of histopathologic examine in cutaneous vasculitis.

目的：评价组织病理学在血管炎诊断中的价值。方法：本文对180例经组织病理诊断为皮肤血管炎的病例，进行系统的对照分析。

Objective: To investigate the visual attention mechanisms of brain through ERP technique by using the "cue-target" experimental paradigm, and the attended range were cued by different Chinese characters cue or region cue; the visual attention mechanisms of brain were investigated through ERP technique. Method:16 healthy young participants were involved. The stimulus trail included "background-cue-target". The background was comprised of three homocentric white circles.

目的：采用&提示-目标&的视觉实验范式，分别以汉字和范围提示不同等级的搜索范围，通过事件相关电位技术，研究视觉注意脑机制：方法：16名在校大学生作为有偿被试，刺激序列为背景-提示-靶刺激：背景由三个同心白色圆形线条组成。

objective:to explore the diagnosis and treatment of gastric stump cancer.method:the clinic datum of 27 cases with gsc were analyzed retrospectively from june 1989 to june 2004.result: the ratio of gsc was 1.8% in homochronous gastric cancer.in 27 cases,the radical operation was performed for 14(53.5%),palliative operation for 9(34.6%)and pass-procedure for 3(11.5%).the one-year,three-year and five-year postoperative survival rate in radical operation was 85.7%, 50% and 35.7%.

目的：分析探讨残胃癌的诊断和治疗方式。方法：对我院1989年6月至2004年6月27例残胃癌患者的临床资料进行回顾性探讨。结果：残胃癌占同期胃癌1.8%，施行根治性手术14例（53.3%），姑息性手术9例（34.6%），由于广泛转移未行手术或仅行剖腹探查术3例(11.5%)。根治性手术病人1，3，5年生存率85.7%，50%，35.7%。

From April 2003 to February 2008, a total of 36 patients (39 limbs) suffered from refractory bone nonunion were studied, there were 22 males and 14 females with an average age of 51.5 years (range, 15~71 years). The nonunion occurred in 19 tibia, 9 humeri, 7 femora, and 4 radius or ulnae. The nonunion experienced operation for 1 to 4 times (average, 2. 5 times). The fracture gaps were4.5~36 mm (average, 16.5 mm) according to X-rays measurement before operation. The shortened length of lower limb was 5-40 mm (average, 21 mm).

方法］2003年4月－2008年2月，共收治四肢难治性骨不连36例39肢，男22例，女14例；年龄15~71岁，平均51.5岁；胫骨19肢，肱骨9肢，股骨7肢，尺、桡骨4肢；治疗骨不连手术次数1~4次，平均2.5次；术前X线片骨不连骨断端距离：4.5~36mm，平均16.5mm；下肢短缩距离：5~40mm，平均21mm。

Objective:To investigate the diagnosis and surgical treatment effect of hydrocephalic neurocysticercosis.

目的：探讨脑积水型脑囊虫病的诊断与外科手术治疗效果。方法：对诊断明确的73例脑积水型脑囊虫病患者实施了脑室或脑池囊虫摘除术，术后进行抗脑囊虫病治疗。

The life cycle assessment method was used to investigate the environmental impacts of metallic copper production with hydrometallurgical and pyrometallurgical processes.

运用生命周期评价(life cycle assessment，LCA)方法，对用火法、湿法两种方法生产金属铜过程的环境协调性进行了研究，得到了铜生产过程中各工序的环境负荷数据。

I think people in this lifetime in a rebirth.

我觉得人在这一生里其实也在轮回。

He had to tie her hands together.

他不得不把她的双手绑在一起。