方法

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

It is based on the analysis of reflectometric interference spectrum and by this means, optical constants, such as extinction coefficient, refractive index, absorption coefficient and thickness, can be calculated with a high precision.

该方法是一种高精度的测量方法，与透射光谱分析法相比，反射干涉测量方法对于薄膜系统的载体要求较小，可以是透明载体，也可以是非透明基底，可以是金属载体，也可以是光纤端面。

Indirect-method is an important method in artificial proof, and reduction to absurdity is one of the effective methods.

在手工证明几何问题的方法中，间接证明也是一种重要的方法，其中反证法是较为有效的方法之一。

By analyzing the spatial relations of urban road network, the abuttal relation between road sections is discussed.(2) By analyzing the cognition process for sign information, the design method based on spatial reasoning is established.

首先对城市道路网的空间关系进行了分析，明确了路段邻接关系的表达；然后通过对标志信息的认知分析，探讨了基于空间推理的道路信息标示设计方法，最后对方法的具体实现进行了说明。3、基于标志性地物的指路标志设计方法研究。

So far, we human beings for the heating of the concept has long been restricted to convection, conduction, radiation three kinds of surface heating in it, in recent years the use of molecular friction between the object itself is heated and heat of the principle of the developed in the dielectric heating (including the high-frequency and microwave heating) has been widely used in industrial heating and drying purposes above, this heating method unlike the aforementioned three kinds of methods, the heat do not penetrate the surface, but will wear the electromagnetic energy directly to transparent objects into heat inside a method, also known as the fourth heating methods

到目前为止，我们人类对于加热之观念一直都被局限于&对流&、&传导&、&幅射&三种表面加热方法而已，近年来利用&被加热物体本身分子磨擦生热之原理&所发展出来之诱电加热被广泛应用于工业加热干燥用途上面，此种加热方法不像前述之三种方法，热量不需要穿透物体表面，而是将电磁波能量直接穿透物体内部转换成热量的一种方法，也被称为第四种加热方式

How to exploit and utilize water resources reasonably, and allocate them scientifically on the basis of the existing cir...

本文针对江苏省连云港市区的实际情况，分别对水资源需求预测方法、优化配置模型和优化配置决策支持系统等问题进行了研究，论文主要内容包括以下几个方面：(1)在分析需水预测分类以及影响因素的基础上，详细介绍了各行业需水预测的常规方法，并采用定额法、回归分析方法和人工神经网络方法对连云港市区2010和2020两个规划水平年的需水量进行了预测分析。

In chapter 2 and 3, experimentally, using the Angular-Resolved high-resolution fast Electron Energy Loss Spectrometer , at the condition of 2. 5 keV incident energy and 50-60 meV energy resolution, we measured the Optical Oscillator Strength Density Spectra for the excitations of 4p, 4s or 3d electron. The oscillator strengths for excitations of the valent shell 4p electron were obtained, and comparisons were done between presently experimental and previously experimental and theoretical results. The experimental results of different groups agree with each other approximately, but the semi-experientially theoretical results do not match with the experimental results. The delayed maximum in the photoabsorption spectra was discussed. It should arise from the transition of 4p→∈d. For the excitation of the inner-valent 4s electron, the discrepancies for the resonant structures in previous electron-impact results and photoionization results were clarified in present work, which confirms again that the fast electron impact method is suitable to measure the optical oscillator strengths. The autoionization Rydberg series 4s〓ns (n=5, 6, 7) and 4s〓nd (n=4, 5, 6, 7) were identified without ambiguity by the measurement at 0°, 2° and 4°scattering angles. The energy levels and natural widths of the excitations of Kr3d and Ar2p inner shell, including optically allowed and forbidden transitions, were determined. The widths of these inner shell excitations are nearly the same, which was interpreted by the Resonant Auger effect .

在第二章和第三章，实验上，使用角分辨的高能量分辨快电子能量损失谱仪，在2.5keV电子入射能量和50-60meV能量分辨下，测量了Kr原子由价壳层4p到内价壳层4s，再到内壳层3d电子激发的光学振子强度密度谱；得到了价壳层4p电子激发束缚态的光学振子强度，与前人实验和半经验理论结果作了细致的比较，说明几家实验是比较符合的，但半经验的理论计算存在问题；分析了光吸收谱中的延迟极大现象，说明在第一电离阈值以上几个eV范围内的极大值源于4p→εd跃迁产生的延迟极大；对于内价壳层4s激发的自电离区，澄清了前人实验中电子碰撞方法和光学方法在共振结构上存在差异的问题，再一次肯定了快电子碰撞方法是获得绝对光学振子强度的一种好方法；通过在非0°散射角的测量（如2°和4°），清楚地标识了4s电子激发的光学禁戒跃迁自电离里德堡系列4s〓ns（n=5，6，7）和4s〓nd（n=4，5，6，7）；通过在0°和4°散射角的测量，观测并标识了几个新的内壳层光学禁戒跃迁能级，得到了Kr原子3d和Ar原子2p内壳层激发态（包括光学允许和禁戒跃迁形成的）的能级位置和自然宽度，用共振俄歇效应解释了这些内壳层激发态（不管是光学允许还是禁戒跃迁产生的）的自然宽度彼此比较接近的原因。

The baber gives a brief introduction to the basic concept of reliability design,brings out,by the example of the design of the shaft which is deformed by the combining of bending and torsion,the...

本文介绍了可靠性设计的基本概念，并以受弯扭组合变形的转轴设计为例，导出转轴可靠性设计的方法和公式，把用可靠性设计方法计算出的结果和用传统设计方法计算出的结果进行比较，说明可靠性设计方法的优越性。

In chapter 1,the basic theory of several computational methods including various ab initio,semiempirical and DFT methods were introduced.

第1章中我们简要介绍了用于研究分子体系的主要计算方法的理论基础，包括各种从头计算方法，半经验方法和密度泛函方法。

B Under linear condition, the dynamic behavior of viscoelastic materials, i. e. their complex modulus, Can be identified simultaneously at diffirent frequencies by the method.

对比以往研究者提出的方法，新方法有两大优点：第一，新方法可辨识粘弹材料非线性动态特性；第二，在线性情况下，用新方法可一次得到多频率点处的复模量值。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。