方法

objectiveto explore the application of carpometacarpal superficial vein graft in the digital replantation.methodthirty cases hospitalized in our hospital from january 2004 to october 2008 were dealed with vessel defect in the digital replantation by carpometacarpal superficial vein graft.resultsthe replanted fingers all survived,the appearance and function were good.conclusionusing carpometacarpal superficial vein graft is a good way to deal with vessel defect in the digital replantation.

目的：探讨腕掌侧浅静脉移植在断指再植中的应用。方法：对30例患者采用腕掌侧浅静脉移植处理断指再植手术中的血管缺损。结果：再植手指均成活，外观和功能良好。结论：对于断指再植中血管缺损的处理采用腕掌侧浅静脉移植是一种很好的方法。

The cadmium stress was set up by 5 concn. treatments at 0, 0.5, 5.0、10.0, 50.0 mg/L to soak D. caryophyllus seeds and fresh leaves resp., the seed germination energy, leaf chlorophyll and isozyme were determined. The low concn.

方法］镉胁迫设5个处理(0、0.5、5.0、10.0、50.0mg/L)，对石竹种子浸泡后测定其发芽率；对新鲜石竹浸泡后测定其叶绿素含量和同工酶。

The P. caudatum were cultured in the straw water with different mass concn. of Cu(superscript 2+), its polysaccharide was extracted and detected by the anthrone-sulfuric method and the effect of Cu(superscript 2+) on the content of polysaccharide in P.

方法］通过用含不同质量浓度Cu(上标 2+)的稻草液培养大草履虫，提取其体内多糖，用蒽酮－硫酸法进行检测，研究Cu(上标 2+)对大草履虫体内多糖含量的影响。

The photosynthesis index of three-year age Cerasus humilises planted in potted experiment were irrigated with different concentration of NaHCO3 solution (100, 200 and 300 mmol/L) was measured on the 7th, 11th, 15th, 21st, 30th and 36th day.

方法］以浓度为100、200、300mmol/L的NaHCO3溶液浇灌3年生欧李苗，在盆栽条件下，于第7、11、15、21、30、36天测定苗木光合作用各项指标。

Objective:This study deals with the diagnosis of cervicobrachial and lumbodynia disease,By adopting Foxpro database and Prolog Languase, disease informatin is compiled to the form applicable to computer language and programme compilation,the cases of disease can be obtained in the process of puting in case of disease ,the differential diagnosis information is entered into database and compiled to different codes at knowledge base ,deduction diagnosis can be achieved through the code conversion and compariso...

颈腰疾病微机专家诊断系统王加宽俞立平乔闯缪天英摘要目的：研究颈腰疾病的计算机诊断。方法：采用Foxpro数据库和Prolog语言将疾病的资料编写成适合计算机语言和程序编写的格式，在病案录入过程中完成病案的获取，将疾病的鉴别诊断资料输入数据库并编写成不同的点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose［1000 mg/(kg1·d)］ for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides ［200 mg／group,tanshinone ［1 500 mg／］ group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射 d-半乳糖［1000 mg/］造成小鼠衰老模型，同时各组分别灌胃生理盐水、枸杞多糖［200 mg/］、丹参酮［1 500 mg/］。42 d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

Methods:The clinical data of 6 cases that received LC combined with EST herapy for cholecystolithiasis and choledocholithiasis were analyzed retrospectively.

目的：总结腹腔镜胆囊切除术＋十二指肠乳头括约肌切开术治疗胆囊、胆总管结石的体会。方法：为6例患者行LC＋EST。

objective: to study congenital choledoch cyst diagnosis, treatment and complication preventing and controlling.

目的：探讨先天性胆总管囊肿的诊断、治疗及并发症的防治。方法：对22 例先天性胆总管囊肿的患者进行分析。

The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

方法］采用苯酚：氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA，运用P2/P8引物扩增CHD基因片段，将PCR产物克隆到T-Vector，利用NCBI的Blast程序，以短趾鹰同源序列为比对参照，将帝企鹅CHD基因片段序列与GenBank中的基因片段序列进行同源性比较分析，鉴定帝企鹅的性别。

MethodThe extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen. The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃, centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.

方法］取800～1 000 mg许树和单叶蔓荆的鲜叶，加液氮研磨后制成提取液，提取液加试剂4 ℃下离心，冰浴后室温下离心，依次添加试剂后室温下离心，即得total RNA样品。

"Yes, now you can give yourself airs," she said, you have got what you wanted.

"对了，您现在高兴了，"她说道，这是您所期待的。

Then the LORD said to me: Rebel Israel is inwardly more just than traitorous Judah.

上主于是对我说：＂失节的以色列比失信的犹大，更显得正义。