方法

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

Objective:Through the establishment of tractive spinal cord injury model on the rat,we discussed the pathophysiological changes and clinical significance of this scathe by the evaluation of magnetic stimulating motion evoked potential and behavioral functional tests.

目的 ：建立大鼠脊髓牵拉性损伤的模型，探讨实验性脊髓牵拉性损伤的病理生理改变及临床意义。方法：双侧椎板切除显露大鼠脊髓的全宽，用模拟神经拉钩特制的牵开器由侧方牵拉脊髓，实现水平方向上的脊髓牵拉性损伤，并用磁刺激运动诱发电位和行为学功能试验指标进行综合评价。

Based on the field experiments in 2000~2004, the yield of 2-year-old herbage was determined from the heading stage to the flowering stage and the relativities between the yield and 3 climate factors (including precipitation, sunlight and temperature) were analyzed by scatter diagram and linear regression method.

方法］通过2000~2004年的田间试验，在抽穗至开花期测定2龄牧草的产量，通过点聚图与线性回归法分析青海老芒麦草产量与3个气候因子的相关性。

The seeds of rice mutants with Ac/Ds elements inserted from rice variety Dongjin were selected and the callus were induced from scutellum of mature embryo. Ca(superscript 2+) with different concn. was added into the selected optimum enrichment, differentiation and rooting medium to study the effect of Ca(superscript 2+) on proliferation, differentiation and rooting of rice callus.

方法］选用水稻品种Dongjin的Ac/Ds插入突变株系的种子，从成熟胚盾片诱导出愈伤组织，在筛选出的最适增殖、分化及生根培养基中添加不同浓度的Ca(上标 2+)，研究Ca(上标 2+)对水稻愈伤组织增殖、分化及生根的影响。

Methods (1) Twenty male SD rats were randomly divided into control group and hypoxic group.

方法(1)将20只雄性SD大鼠随机分为对照组和低氧组。

Objective: To recommend an improved method of prostate massage , which is safe, effective, simple and tolerable, and may increase the expressed prostatic secretin success rate and promote the efficiency of the prostate massage.

目的 ：介绍一种改进的安全、有效、简单、患者耐受性好的侧俯站位平行半蹲式经直肠前列腺按摩法，以提高前列腺按摩成功率。方法：2 6 1例慢性前列腺炎患者，年龄 1 9～ 6 7岁，平均 37.5岁，病程 3个月～ 2 0年，平均2 。5年。

The compositions and contents of amino acids in the neck, leaf and seed of Alfalfa were analyzed with amino acid auto analyzer.

方法］利用氨基酸自动分析仪测定分析苜蓿的茎、叶和种子的氨基酸成分和含量。

The features of this method are, it adopted a vision matching method to map some color appearance factors between self-luminous body and reflector surface colors, but without the complexity of using any color appearance model.

该方法的特点是采用"视觉匹配"方法，在反射体表面色和自发光体之间映射一些色貌因素，但没有使用任何复杂的色貌模型。

A self-supervision system is built comprised of self-record, self-score, and self-question with the learners and the activity as the subject of cognition.

本研究运用系统论的方法，从内容、模式、方法、监控以及评价等五个有机联系的子系统出发，建构网络教育中道德自主学习的科学体系。

Methods: A total of 78 breast cancer patients were investigated on their anxiety state by filling out self-rating anxiety scale.

方法］采用问卷调查法，应用焦虑自评量表对78例乳腺癌根治术病人的焦虑状态进行调查分析。

It is suggested that TDS can be used as a new method for the analysis of pedogenesis and climatic change.

总之，热处理过程中磁化率的变化能非常灵敏地反映磁性矿物的转化情况，而且，这种转化往往与样品所经历的土壤化作用强度密切相关，因此，TDS可做为分析成土作用强度和古气候变化的一种新方法。

Therefore, in this chapter, I will research into the conflict and contradiction of the paternity. Also, in this novel, the father's behavior of "He who loves well chastises well." is quite suspicious, one of the reasons is that the father thought his son belonged to him. And then, this thesis quoted Lacan's theory to touch upon that someone who has accepted the concept of patriarchal society, he will be influenced for his whole life. That also reveals that human being has no choice sometimes in this society.

因此本章会先就父子关系的冲突与矛盾做深入的探讨，小说中的父亲似有对儿子爱之深责之切的行为，其原因可能在於父亲将儿子视为所有物所致；其次，论文将引用拉冈相关理论，讨论在父权社会中，不论身分为何，只要是已受父权社会观念根深蒂固影响的人物，终生都将受其所苦，此也揭示出人生存於社会上的无奈。