方法

Based on how various national and international performance evaluation systems be used in cooperation, and after introducing of various performance evaluation systems, analyzing the advantage and disadvantage of each system, especially introducing the advantage and disadvantage of balance scoring card, economic added value, performance evaluation guide inside cooperation and other evaluation system, meanwhile, reviewing the performance evaluation systems used by Yingkou Port in the past, introducing the performance evaluation system used by Yingkou Port at present, this article is to design a new performance evaluation system for Yingkou Port after disusing and referencing above systems.

本文围绕当前国内和国外的各种业绩评价方法如何用于企业集团的绩效评价，通过对各种评价方法予以介绍，并对其特征和优缺点进行分析，尤其是介绍平衡计分卡、经济增加值、企业集团内部效绩评价指导意见等评价方式的优缺点，并对营口港沿用的业绩评价方法进行回顾，介绍营口港现有的业绩评价方法，来探讨借鉴各评价方法来重新改进营口港业绩评价体系。

Many research tools and methods for ship collision analysis have been developed since Minorsky's inaugurating study in this field, including empirical method, simplified analytical method, experimental method, finite element method, and so on.

从1959年Minorsky在船舶碰撞领域做出开创性工作以来，到现在已经发展出多种研究方法用于船舶碰撞分析，包括经验方法、简化的解析方法、试验方法和有限元仿真方法等。

Then a dynamic inversion tracing control method and a dynamic inversion fuzzy integrated control method are used to design depth control systems for a torpedo respectively. The simulation results illustrate that dynamic inversion fuzzy integrated control method can solve the problem of torpedo depth control more satisfactorily than the dynamic inversion tracing control method can.

提出了基于动态逆的跟踪控制方法和动态逆模糊集成控制方法，并分别将这两种方法用于鱼雷深度控制中，通过仿真说明动态逆模糊集成控制方法比基于动态逆的跟踪控制方法更好地解决了鱼雷的深度控制问题。

In chapter three, with the effective combination of the prior preference, posterior preference and interactive method, and based on the theory of satisficing decision making and goal programming, an interactive method for solving static or dynamic multicriteria programming is devised. In the method, by constructing the simplexes in the set of parameters and transforming them, the local preference information is interactively quested in th process of decision making. The prior and posterior preference are used to speed up the process of decision making and overcome the false convergency which may be occured in pure interactive algorithms. This method can be used to cope with linear, nonlinear and dynamic multicritcria programming.

在第3章多目标数学规划的参数单纯形方法中，通过多目标数学规划的验先偏好方法，交互式方式与验后偏好方法的有效组合，在满意决策和目标规划的理论基础上提出了一种求解多目标静态及动态规划的交互式方法，该方法通过在参数集中构造单纯形并进行其变换，在决策过程中交互地索取决策者的局部偏好信息，并利用决策者的验先偏好来提高决策过程进行的速度，及利用决策者的验后偏好来克服纯交互式算法中可能出现的假收敛现象。

Any letters beyond these requirements are to be excluded, so if the decree specifies k comparisons then the new alphabet will contain the first k+1 lowercase letters of the current alphabet. Create a class Alphabet that contains the method choices that takes the decree as input and returns the number of possible new alphabets that conform to the decree. If more than 1,000,000,000 are possible, return -1. Definition Class: Alphabet Method: choices Parameters: string Returns: int Method signature: int choices Constraints decree contains between 1 and 25 characters inclusive.

我注：如上面的BAA有3个字符，那么新表就有4个，也就是'd'后面的全被忽略创建一个名为Alphabet的类，此类含有一个名为choices方法，choices方法以&规定&作为参数输入，然后返回所有符合这个规定的新字母表的可能数，如果大于1，000，000，000可能，就返回-1 定义：类名：Alphabet 方法：choices 参数类型：string 返回值类型：int 方法签名：int choices (确保choices为公有方法)参数条件：规定中的'B'和'A'总和必须大于等于1，小于等于25 举个例输入：&BAA&返回：3 解释：因为根据规定'a'在'b'的前面，'b'在'c'的后面，'c'在'd'的后面，所有符合这个规定的新字母表有3个：adcb、dacb和

According to the common process of software metric,a research on software reliability metric which is based on the real defect is made firstly.Then the quantitative defect statistic method is applied which is based on ODC.This method can describe the information of defect well and can be used for process improvement.At last, the qualitative method of defect analysis is showed,which can dredge up underlying information.

首先将缺陷度量方法按照软件度量的一般方法进行研究和探讨；其次，以正交缺陷为基础，提出了定量的缺陷统计方法，这种方法能够更好地描述缺陷的性质，对相关信息可进行分类统计、分析，为过程改进提供依据；最后，提出了一些定性的缺陷分析方法，可充分挖掘缺陷的潜在信息。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

A quasi one-dimensional, time dependent system model based on the parallel compressor theory was used to compute the effect of inlet distortion on turbine engine stability; Through Modifying steady state continuum equation, momentum equation and energy equation, the effect of power extraction and additional bleed on turbine engine running line was computed. Unsteady state continuum equation, momentum equation and energy equation were solved for computing the effect of acceleration on turbine engine running line.

其中，进气畸变对发动机气动稳定性影响的计算采用平行压气机模型，基于李亚普诺夫理论的方法完成；附加引气、功率提取对发动机气动稳定性的影响采用通过对发动机转子间质量、动量、能量守恒方程进行修正的方法进行；加速过程对发动机气动稳定性的影响采用欧拉方法求解动态的质量、动量、能量守恒方程的方法完成；最后采用&层迭&的方法进行各种降稳因素的综合评估。

For frequency information fluid identification technology:the basic theory of fluid identification based on frequency information has been introduced,for inner and external factor affecting frequency,some steps have been proposed to attain reserved amplitude and frequency analysis data with high resolution,high S/N ratio;through the comparison of time-frequency analysis method, the high accuracy time-frequency analysis method has been selected to extract time-frequency attributes;the analysis method flow of common single frequency fluid identification has been proposed,according to the data of full frequency band,several anomaly zone modes of common single frequency attribute have segregated; incorporating converted wave and compression wave,fluid identification method based on PS wave combined time-frequency analysis has been proposed;the AVO property of various fluid type in frequency domain has been studied;the generalized fourier analysis method,Proni absorption filter has been introduced,and researched for anti-noise and for the seismic processing interpretation flow.The above technology have been applied to fluid identification of organic reef in WBT area in the east of Sichuan and fluid identification of clastic rock in GUANGAN area in the middle of Sichuan,the results show that the above methods are effective.

基于频率信息的流体识别技术方面：介绍了利用频率信息流体识别的基本理论，针对影响频率的内、外在因素，提出一些针对措施，以获得高分辨率、高信噪比的保幅、保频分析数据；通过时频分析方法的比较，选取高精度的时频分析方法提取时频属性；提出共单频属性进行流体识别的分析方法流程，结合全频带数据，分出几种共单频属性异常带模式；联合转换波资料和纵波资料，提出基于纵横波联合时频分析进行流体识别方法；研究了频率域内不同流体类型AVO现象；引进一种广义傅立叶分析方法：Proni吸收滤波，研究其抗噪性以及进行地震处理解释的流程；将这些技术应用川东北五百梯区块进行生物礁储层流体的识别和川中广安陆相碎屑岩的流体识别，取得较好的效果。

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At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。