方法

The results showed that in evaluation of the method by detecting 50 RHD 1227A positive and 50 RHD 1227A negative individuals, the genotyping method displayed a sensitivity of 100% and a specificity of 100%; in evaluation of the method by detecting 33 DEL positive and 89 DEL negative individuals, the sensitivity was 100%, however, there were two serologically negative samples which were confirmed as positive using genotyping method. After re-testing these two samples with serological method and sequence analysis, it was found that original serological method gave false negative results and genotyping method still showed 100% specificity. The minimal target DNA concentration of this genotyping method is 8.13 ng/μl.

结果表明：在50例RHD 1227A阳性和50例RHD 1227A阴性的Rh阴性样本中基因分型方法的灵敏度和特异性都是100％；在33例DEL阳性样本和89例DEL阴性的样本中，基因分型方法的灵敏度为100％，有2例样本血清学结果为阴性而基因分型结果为阳性，重新用血清学方法和序列分析方法复核这2例样本，发现2例都是血清学漏检，因而基因分型方法的特异性是100％。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

Four aspects of research achievements include:(1)several imaging correction methods and strained estimation methods for biological tissues elasticity imaging were investigated as well as elasticity reconstruction and phantom, in vitro and in vivo elasticity imaging experiments;(2)in the fields of biological microsystem and perfusion evaluation for microvascular system, three kinds of microbubbles whose shell are albumin, sugar ester and surfactant respectively were designed and produced, then the methods to estimate the thickness of their shells were presented, moreover, the interacting mechanism of ultrasound contrast agents and tissues, as well as protein drug delivery under the condition of ultrasound sonification were studied in the molecular level;(3) At the aspect of high intensity focused ultrasound therapy system, we designed and constructed the 256-element HIFU transducers using 1-3 composite materials. And furthermore we developed the integrated HIFU system with 256-element HIFU transducers. And we made preliminary and important progresses in the research of HIFU bioeffect, lesion estimation, techniques of monitoring and imaging and characterizing of structure of HIFU transducer;(4)The real-time method of voice source regeneration based on compensative regulation of physiological parameters was investigated in the field of medical rehabilitation. We addressed the physiology and biophysics of vocalization, modeled voice source regeneration based on compensative regulation mechanism, and put forward an integrated evaluation about the speech rehabilitation and the regenerated voice source; Sixty-one archived journal papers were published, as well as ten in international journals, and eighteen publications were embodied by SCI.

中文摘要：成果包括：（1）在生物组织弹性成像方面，研究了多种图像校正方法和应变估计方法，进行了弹性重构和仿体、离体及在体超声弹性成像实验研究；（2）在生物微系统和微血管血流灌注成像方面，制备了三种不同包膜超声造影微泡，研究了纳米包膜厚度检测与估计方法以及不同纳米包膜造影微泡断裂与药物释放机制，并在分子层次上开展了超声与生物组织相互作用机制以及蛋白质药物超声乳化微囊包裹的研究；（3）在阵列式高强聚焦超声治疗与监控成像技术方面，设计制作了新的HIFU换能器，解决了256阵元单焦点和多焦点合成HIFU治疗系统关键技术问题，并在HIFU生物效应与创伤性评价方法、HIFU过程监控成像技术和薄层复合结构与HIFU 换能器界面特性评价等方面的研究取得了初步而重要的进展，具有重要应用前景；（4）在语音听觉系统特别是嗓音康复方面，研究了基于发声系统补偿调节机制的实时多模式嗓音源再生方法和发声生理，建立基于神经、肌肉和空气动力系统的综合补偿调节模型，提出了相应的评价系统。

Then, using nondeterminism analysis method, at the based of summering reliability basic theory and present reliability assessment method, aiming at the limits of present reliability analysis method, the improved response surface method of higher pile-column bridge pier structural system is presented for reliability calculation by introducing response surface method.

此外，采用非确定性分析方法，在总结可靠度基本理论及现有可靠性分析常用方法的基础上，针对现有方法的局限性，引入响应面方法，建立高桥墩-桩基结构体系稳定的改进响应面方法。

On the basis of previous approaches to query expansion, this paper proposes a new approach to query expansion based on set theory,which combines three traditional methods—thesauri,automatic relevance feedback and the related words of the question type.

通过分析已有的查询扩展方法，提出了基于集合论的查询扩展新方法。它结合了3种传统的查询扩展方法：语义词典法、自动相关反馈法和问题类型词。实验结果表明该方法在Web检索方面是有效并且优于传统的方法。

The new statistical analysis methods are mostly based on mixed linear model approaches for estimating variances and covariances, unbiasedly predicting random effects, analyzing conditional variaces and effets, and composite interval mapping.

基于混合线性模型的分析原理的统计分析方法包括方差和协方差估算方法、随机效应的无偏预测方法、条件遗传方差和条件遗传效应的分析方法、复合区间作图方法等。

With the support of a major research grant of Zhejiang Province (No. 001101027) and the Plan of Promoting Education of Excellence of Zhejiang University in the 21st Century, this dissertation is devoted to address the above problems and fabricate an L-band EDFA with low cost, high performance and technological innovation.First the mechanism of an erbium doped fiber amplifier is introduced. Various theoretical models based on classical rate propagation equations are summarized and compared for their applications. We use a modified numerical model based on the Giles model, which agrees with the experimental results well. This serves as the theoretical base for the whole thesis and will be used to optimize the characteristics of EDFAs. A novel method based on a map of noise figure and gain is proposed to analyze efficiently the dependence of the noise figure and gain for EDFAs on the EDF length and the pump power. The characteristics of various EDFAs are studied and compared.Secondly, some important parameters are introduced to describe the characteristics of EDFAs. The methods and the experimental setups for measuring the gain and noise figure of an EDFA are emphasized. We introduce how to measure the characteristics of each component of an EDFA, especially the gain and absorption coefficient of an erbium doped fiber.Thirdly, four types of simple and novel L-band EDFA structures with low noise, high gain and low gain ripple are proposed to satisfy the requirement of a DWDM system. They can be classified into two categories. In the first category, a single ASE pumping is served asa secondary pump. This category includes the following three types. Type: a new construction using a fiber Bragg grating in an unpumped EDF section at the input side. Type: a new structure of L-Band EDFA with two-stage pumps, which utilizes the forward ASE as a second pump.

本论文本着科技创新、服务于生产实践的精神，以设计价格低廉，性能优越、结构合理简单的L-band EDFA为目标，依托浙江省科技厅重大科学基金资助项目(001101027)和浙江大学振兴教育计划资助的全光网络的搭建—《光通信技术》课程实验建设项目，展开了如下工作：首先，介绍了掺铒光纤放大器的工作原理，归纳总结了基于速率方程基础的EDFA的各种理论模型，分析比较了各个模型的特点和适用场合，最终选择完善的Giles理论模型作为本论文分析设计EDFA的理论基础；针对实际实验条件提出了修正的EDFA模型的数值计算方法；创新地提出了增益—噪声系数全局分析法，直观有效地分析了EDFA的增益和噪声系数与掺铒光纤长度和泵浦功率的依赖关系，并对各种EDFA的性能作了全面的比较；第二，介绍了掺铒光纤放大器特性参数的定义与测量方法，重点介绍了噪声系数和增益系数的测量方法及实验装置；介绍了组成掺铒光纤放大器各组件的特性测量方法，重点介绍了测量掺铒光纤参数的方法和实验装置；第三，针对WDM系统对L-band EDFA谱平坦、低噪声、高增益的要求，我们创新地提出了四种不同的L-band EDFA结构，按设计思路可以分为两大类：一类是基于单抽运二级泵浦法，这一类包括在未泵浦掺铒光纤的输入端插入一根布拉格光纤光栅的两段L-band的EDFA的新结构；基于前向ASE光作为二级泵浦源推动下一级EDF工作的泵浦分配、两段级联L-band的EDFA的新结构；基于单根光纤光栅、泵浦分配、两段级联的EDFA；另一类则是基于同时应用前后向C-band ASE作二级泵浦源的双抽运法，如基于前后向ASE光作为二级泵浦源的三段级联L-band的EDFA的新结构。

The new method for seismic attribute analysis colligates information of seismic,logs and geology by crossplot and broadcast and cluster with the methodology of science instead of analysis with handcraft and eyeballing.With the method,seismic attribute analysis plays an important role in subtle traps exploration. \r\n

指出新型的地震属性分析方法一改以往的手工、目测分析方法，充分利用了二维、三维及多维交汇方法，为属性分选、分析提供了便利的操作手段；新型的地震属性方法还运用科学分类方法，综合地震、测井、地质信息，把剖面、平面及分类结果结合到一起，通过三维一体的相互信息映射，在储层预测中的目标初选及目标分析中起到了举足轻重的作用，真正做到了属性分析的科学性、实用性。

Textile colorfastness to crocking is very important, and there are differences among test methods in different countries. The test results are influenced by different test methods. In order to avoid the "trade rubs" among different countries, it is necessary to know the correlation among test results of different test methods. In this paper, Chinese GB(GB/T 3920-51997), AATCC(AATCC TM 82004) and JIS(JIS L 0849-1996) test methods of colorfastness to crocking were introduced.

纺织品耐摩擦色牢度是重要的检测指标，各国的标准和测试方法有一些差异，而不同的测试方法对测试结果有一定的影响，为了解不同测试方法测试结果的相关性，以避免贸易摩擦，本文介绍了耐摩擦色牢度的我国国标(GB/T 3920 5 1997)、美国纺织化学家及染色家协会标准(AATCC TM8 2004)、日本标准工业协会标准(JIS L 0849 1996)等三个测试方法标准，通过对相同试样的三种测试方法的测试，探讨了评定值之间的相互关系。

The process features and shape features of the electronic part are classified and defined, and then the feature model library is built, which enables rapid input of the part information. The rules and methods of stamping process design are summarized to build the stamping process library, so that the stamping process can be designed rapidly and intelligently. Furtherly, the process design rules and methods in process station layout of multi-station progressive die as well as the methods of decompounding and compounding process and the expression method of the process station layout are concluded and summarized, and then the rule library and case library of process station layout are built. Designing the process station layout rapidly is carried out based on rules and cases. At last the rapid design function of process station layout is validated through simulating the rapid designing layout system of multi-station progressive die on Pro/E 3D platform.

主要研究成果有：对电子产品冲压件的工艺特征与形状特征进行了分类和定义，建立了特征模型库，实现了冲压件信息的快速输入；归纳了冲压工艺设计规则和方法，建立了冲压工艺知识库，实现了冲压工艺快速与智能化设计；归纳总结了多工位级进模工步排样中的工序设计原则和方法，工序分解与工序组合方法，工步排样知识的表示方法，建立了工步排样规则库和实例库，利用基于规则和实例的推理方法，实现了工步排样的快速设计；在Pro/E三维软件平台上开发了电子产品多工位级进模工步排样快速设计系统，系统通过实例验证了工步排样的快速设计功能。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。